Epigenome formation and cellular differentiation in mouse embryogenesis

2012 Fiscal Year Final Research Report

• Project Area: The germline: its developmental cycle and epigenome network
• Project/Area Number: 20062003
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: The University of Tokyo
• Principal Investigator: TANAKA Satoshi 東京大学, 大学院・農学生命科学研究科, 准教授 (90242164)
• Co-Investigator(Kenkyū-buntansha): OHGANE Jun 東京大学, 大学院・農学生命科学研究科, 助教 (50313078)
• Co-Investigator(Renkei-kenkyūsha): SHIOTA Kunio 東京大学, 大学院・農学生命科学研究科, 教授 (80196352)
• Project Period (FY): 2008 – 2012
• Keywords: DNAメチル化 / マウス / 胚盤胞 / 内部細胞塊 / 栄養外胚葉 / 栄養膜細胞 / 胎盤

Research Abstract

We analyzed DNA methylation profiles of mouse embryonic stem cells (ESCs) and trophoblast stem cells (TSCs), and compared those with DNA methylation profiles of some somatic tissues. Consequently, we identified genomic regions that show methylation statuses specific to each stem cell line. By using DNA methylation profile as one of the indexes, we revealed that the TSCs derived from blastocysts produced by somatic cell nuclear transfer are indistinguishable from normal TSCs. We also showed that trophoblast-specific DNA methylation occurs after the segregation of the trophectoderm and inner cell mass in the mouse periimplantation embryo.

Research Products

• [Journal Article] Trophoblast-specific DNA methylation occurs after the segregation of the trophectoderm and inner cell mass in the mouse periimplantation embryo (2012)
  • Author(s): Nakanishi, M., Hayakawa, K., Nakabayashi, K., Hata. K., Shiota, K., and Tanaka, S
  • Journal Title: Epigenetics Volume: 71 Pages: 173-182
  • Peer Reviewed
• [Journal Article] Re-investigation and RNA sequencing-based identification of genes with placenta-specific imprinted expression (2012)
  • Author(s): Okae, H., Hiura, H., Nishida, Y., Funayama, R., Tanaka, S., Chiba, H., Yaegashi, N., Nakayama, K., Sasaki, H,, and Arima, T
  • Journal Title: Hum Mol Genet Volume: 21 Pages: 548-558
  • Peer Reviewed
• [Journal Article] 栄養膜幹細胞 (2011)
  • Author(s): 中西もも,田中智
  • Journal Title: 臨床婦人科産科 Volume: 65 Pages: 260-265
• [Journal Article] Non-CpG methylation occurs in the regulatory region of Sry gene (2011)
  • Author(s): Nishino, K., Hattori, N. Sato, S., Arai, Y., Tanaka, S., Nagy, A., and Shiota, K
  • Journal Title: J. Reprod. Dev Volume: 57 Pages: 586-593
  • Peer Reviewed
• [Journal Article] DNA Methylation-dependent modulator of Gsg2/Haspin gene expression (2011)
  • Author(s): Sato, S., Maeda, C., Hattori, N., Yagi, S., Tanaka, S., and Shiota, K
  • Journal Title: J. Reprod. Dev Volume: 57 Pages: 526-533
  • Peer Reviewed
• [Journal Article] Enrichment of short interspersed transposable elements to embryonic stem cell-specific hypomethylated gene regions (2010)
  • Author(s): Muramoto, H., Yagi, S., Hirabayashi, K., Sato, S., Ohgane, J., Tanaka, S., and Shiota, K
  • Journal Title: Genes Cells Volume: 15 Pages: 855-865
  • Peer Reviewed
• [Journal Article] Genome-wide DNA methylation profile of tissue-dependent and differentially methylated regions (T-DMRs) residing in mouse pluripotent stem cells (2010)
  • Author(s): Sato, S., Yagi, S., Arai, Y., Hirabayashi, K., Hattori, N., Iwatani, M., Okita, K., Ohgane, J., Tanaka, S., Wakayama, T., Yamanaka, S., and Shiota, K
  • Journal Title: Genes Cells Volume: 15 Pages: 607-618
  • Peer Reviewed
• [Journal Article] Trophoblast cell lineage in cloned mouse embryos (2010)
  • Author(s): Oda, M., Shiota, K., and Tanaka, S
  • Journal Title: Dev. Growth Differ Volume: 52 Pages: 285-291
  • Peer Reviewed
• [Journal Article] Interplay between DNA methylation, histone modification and chromatin remodeling in stem cells and during development (2010)
  • Author(s): Ikegami, K., Ohgane, J., Tanaka, S., Yagi, S., and Shiota, K
  • Journal Title: Int. J. Dev. Biol Volume: 53 Pages: 203-214
  • Peer Reviewed
• [Journal Article] Resistance to 5-aza-2'-deoxycytidine in genic regions compared to non-genic repetitive sequences (2010)
  • Author(s): Lim, H.W., Iwatani, M., Hattori, N., Tanaka, S., Yagi, S., and Shiota, K
  • Journal Title: J. Reprod. Dev Volume: 56 Pages: 86-93
  • Peer Reviewed
• [Journal Article] Establishment of trophoblast stem cell lines from somatic cell nuclear-transferred embryos (2009)
  • Author(s): Oda, M., Tanaka, S., Yamazaki, Y., Ohta, H., Iwatani, M., Suzuki, M., Ohgane, J., Hattori, N., Yanagimachi, R., Wakayama, T., and Shiota, K. (OM and TS contributed equally)
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 106 Pages: 16293-16297
  • Peer Reviewed
• [Journal Article] DNA methylation profile of tissue-dependent and differentially methylated regions (T-DMRs) in mouse promoter regions demonstrating tissue-specific gene expression (2008)
  • Author(s): Yagi, S., Hirabayashi, K., Sato, S., Li, W., Takahashi, Y., Hirakawa, T., Wu, G., Hattori, N., Hattori, N., Ohgane, J., Tanaka, S., Liu, X.S., and Shiota, K
  • Journal Title: Genome Res Volume: 18 Pages: 1969-1978
  • Peer Reviewed
• [Presentation] Trophoblast-specific DNA methylation occurs after the segregation of trophectoderm and inner cell mass in mouse periimplantation embry (2011)
  • Author(s): Nakanishi, M.O., et al.
  • Organizer: 国際生殖シンポジウム「生殖生物学の革命と展望:体外受精、胚性幹細胞、エピジェネティクスの展望」
  • Place of Presentation: 岩手
  • Year and Date: 20110913-15
• [Presentation] Trophoblast-specific DNA methylation occurs after the segregation of trophectoderm and inner cell mass in mouse preimplantation embryo (2011)
  • Author(s): Nakanishi, M.O., et al.
  • Organizer: The 2nd World Congress on Reproductive Biology
  • Place of Presentation: Cairns, Australia
  • Year and Date: 2011-10-10
• [Presentation] Difference in the DNA methylation status arises after segregation of trophoblast and embryonic cell lineages (2011)
  • Author(s): Tanaka, S.
  • Organizer: 国際生殖シンポジウム「生殖生物学の革命と展望:体外受精、胚性幹細胞、エピジェネティクスの展望」
  • Place of Presentation: 岩手
  • Year and Date: 2011-09-14
• [Presentation] Establishment of cell lineage-specific DNA methylation profile follows the morphological differentiation of trophoblast and embryonic cell lineages (2010)
  • Author(s): Nakanishi, M.O., et al.
  • Organizer: 43rd Annual Meeting of the Society for the Study of Reproduction
  • Place of Presentation: Milwaukee, USA
  • Year and Date: 2010-07-31
• [Presentation] Normalcy of trophoblast stem cells derived from cloned mouse embryos (2010)
  • Author(s): Tanaka, S
  • Organizer: The First SKLRB Symposia on Frontiers in Periimplantation Biology
  • Place of Presentation: 北京
  • Year and Date: 2010-05-12
• [Presentation] Is trophoblast cell lineage abnormal in cloned mouse embryos? (2009)
  • Author(s): 田中 智
  • Organizer: 第42回日本発生生物学会大会
  • Place of Presentation: 新潟
  • Year and Date: 2009-05-31
• [Presentation] マウスES細胞でのLin28の機能解析 (2008)
  • Author(s): 姫野 絵美,他
  • Organizer: 第146回日本獣医学会学術集会
  • Place of Presentation: 宮崎
  • Year and Date: 20080924-26
• [Presentation] 栄養膜幹細胞の分化に伴うDNAメチル化プロフィール形成 (2008)
  • Author(s): 吉岡 徹哉,他
  • Organizer: 第101回日本繁殖生物学会大会
  • Place of Presentation: 福岡
  • Year and Date: 20080918-20
• [Presentation] マウスプロラクチン遺伝子ファミリーのDNAメチル化解析 (2008)
  • Author(s): 早川 晃司,他
  • Organizer: 第101回日本繁殖生物学会大会
  • Place of Presentation: 福岡
  • Year and Date: 20080918-20
• [Book] Isolation and manipulation of mouse trophoblast stem cells. Curr. Protoc. Stem Cell Biol. 7 (2008)
  • Author(s): Himeno, E., Tanaka, S., and Kunath, T
  • Total Pages: 1E.4.1-1E.4.27
• [Remarks] 雑誌論文(11)に関する研究科プレスリリース
  • URL: http://www.a.u-tokyo.ac.jp/topics/tanaka090826.html