2012 Fiscal Year Final Research Report
Bio-π-space inphotoreceptive proteins:Spectroscopic investigationand creation of new functions
| Project Area | Emergence of highly elaborated pai-space and its function |
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| Project/Area Number |
20108014
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| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
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| Allocation Type | Single-year Grants |
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| Review Section |
Science and Engineering
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| Research Institution | Nagoya Institute of Technology |
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Principal Investigator |
KANDORI Hideki 名古屋工業大学, 工学研究科, 教授 (70202033)
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| Project Period (FY) |
2008 – 2012
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| Keywords | π空間 / 生体分子 / 発色団 / 赤外分光 / アミノ酸変異 |
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| Research Abstract |
Highly elaboratedπ一space can be seen in biomolecules such as proteins, where specific chemical reactions occur in protein environment. In this study, we aimed at revealing the mechanism of such specific chemical reactions in photoreceptive roteins. By combining genetic engineering and various spectroscopic methods, we revealed the mechanism of color tuning and chemical reactions. Of particular notice is the structural study of primate color visual pigments, and the discovery ofthe positive correlation between hydrogen-bonding strength ofintemal water molecules and proton pump fUnction. We also attempted to create new fUnction in bio-π-space, and achieved a protein performing an inward proton tran sport.
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