2012 Fiscal Year Final Research Report
Regulation of gene expression and quality control via mRNA 3'-end processing
| Project Area | Diversity and asymmetry achieved by RNA program |
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| Project/Area Number |
20112005
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| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Nagoya City University |
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Principal Investigator |
HOSHINO Shinichi 名古屋市立大学, 大学院・薬学研究科, 教授 (40219168)
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| Co-Investigator(Kenkyū-buntansha) |
HIROSE Teturou 産総研, バイオメディシナル, 研究チーム長 (30273220)
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| Project Period (FY) |
2008 – 2012
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| Keywords | mRNAプロセシング / ポリA鎖分解 / デアデニレーション / mRNA品質管理 |
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| Research Abstract |
Based on mRNA deadenylation mechanism originally uncovered by us, we have newly identified the following mechanisms of the posttranscriptional gene regulation and RNA quality control. (i) deadenylation mediated by mRNA 3’ UTR cis elements (URE, CPE) and negative regulation of gene expression, (ii) stress-induced global stabilization of mRNA poly(A) tails and formation of stress granules, (iii) mRNA polyadenylation mediated by the non-canonical poly(A) polymerase and positive regulation of gene expression, (iv) quality control of aberrant nonstop mRNA. We also verified quality control mechanism of the nuclear histon mRNA.
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[Journal Article] Biological role of the two overlapping poly(A)-binding protein interacting motifs 2 (PAM2) of eukaryotic releasing factor eRF3 in mRNA decay2012
Author(s)
Osawa, M., Hosoda, N., Nakanishi, T., Uchida, N., Kimura, T., Imai, S., Machiyama, A., Katada, T., Hoshino, S., Shimada, I
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Journal Title
RNA
Volume: 18
Pages: 1957-1967
Peer Reviewed
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[Journal Article] Anti-proliferative protein Tob negatively regulates CPEB3 target by recruiting Caf1 deadenylase2011
Author(s)
Hosoda, N., Funakoshi, Y., Hirasawa, M., Yamagishi, R., Asano, Y., Miyagawa, R., Ogami, K., Tsujimoto, M., Hoshino, S
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Journal Title
EMBO J
Volume: 30
Pages: 1311-1323
Peer Reviewed
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[Journal Article] Identification of the cis-acting and trans-acting determinants of noncoding RNA MALAT-1 for the nuclear speckles localization2011
Author(s)
Miyagawa, R., Mizuno, R., Nakamura, Y., Ijiri, K., Rakwal, R., Shibato, J., Masuo, Y., Hirose, T., Akimitsu, N
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Journal Title
RNA
Volume: 18
Pages: 738-751
Peer Reviewed
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[Journal Article] Thymus-specific noncoding RNA, Thy-ncR1, is a cytoplasmic riboregulator that facilitates hUPF1-dependent degradation of MFAP4 mRNA in specific T-cell leukemia cells2010
Author(s)
Aoki, K., Harashima, A., Sano, M. Yokoi, T., Nakamura, S., Kibata, M. Hirose, T
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Journal Title
BMC Molecular Biology
Volume: 11
Pages: 99
Peer Reviewed
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[Journal Article] Quantitative characterization of TOB interactions provides the thermodynamic basis for translation termination-coupled deadenylase regulation2010
Author(s)
Ruan, L., Osawa, M., Hosoda, N., Imai, S., Machiyama, A., Katada, T., Hoshino, S. and Shimada I
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Journal Title
J Biol Chem
Volume: 285
Pages: 27624-27631
Peer Reviewed
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[Journal Article] Transcripts of unknown function in multiple-signaling pathways involved in human stem cell differentiation2009
Author(s)
Kikuchi, K., Fukuda, M., Ito, T., Inoue, M., Yokoi, T., Chiku, S., Mitsuyama, T., Asai, K., Hirose, T., Aizawa, Y
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Journal Title
Nucl Acids Res
Volume: 37
Pages: 4987-5000
Peer Reviewed
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[Journal Article] Upf1 potentially serves as a RING-related E3 ubiquitin ligase via its association with Upf3 in yeast2008
Author(s)
Takahashi, S., Araki Y., Ohya Y., Sakuno T., Hoshino S., Kontani, K., Nishina H., and Katada, T
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Journal Title
RNA
Volume: 14
Pages: 1-9
Peer Reviewed
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[Presentation] MENβ Noncoding RNA-dependent and -independent Steps Required for Nuclear Paraspeckle Formation2011
Author(s)
Naganuma, T., Nakagawa, S., Kawaguchi, T., Aoki, K., Sasaki, YF., Goshima, N., Hirose, T
Organizer
RNA2011
Place of Presentation
Kyoto
Year and Date
2011-06-15
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[Presentation] Rapid protein production of immunomodulatory chemokine, RANTES, in a multifunctional T cell subset2010
Author(s)
Harashima, A., Toraya, T., Suzuki, M., Yamamoto, M., Hirose, T., Yamazaki, F., Nakamura, S., Kibata, M
Organizer
Tchreg, is coupled with 3'-UTR-mediated mRNA stabilization
Place of Presentation
Kobe
Year and Date
2010-12-07
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[Presentation] MALAT-1 noncoding RNA Regulates Cell Mobility by Modulating Gene Expressions2010
Author(s)
Tano, K., Okada, T., Shibafuji, J., Masuo, Y., Randeep, R., Mizuno, T., Ijiri, K., Hirose, T., Akimitsu, N
Organizer
75^<th> Cold Spring Harbor Symposium
Place of Presentation
NY, USA
Year and Date
2010-06-02
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[Presentation] Alternative 3' end processing of long noncoding RNAs is the underlying regulatory mechanism of nuclear paraspeckle organization2010
Author(s)
Naganuma, T., Nakagawa, S., Kawaguchi, T., Aoki, K., Sasaki, YF., Goshima, N., Hirose, T
Organizer
RNA2010
Place of Presentation
Seattle, USA
Year and Date
2010-05-26
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[Presentation] Roles of Noncoding RNAs in Formation and Function of Nuclear Paraspeckles2010
Author(s)
Naganuma, T., Nakagawa, S., Tanigawa, A., Sasaki, YF., Goshima, N., Hirose, T
Organizer
19th CDB meeting: RNA Sciences in Cell and Developmental Biology
Place of Presentation
Kobe
Year and Date
2010-05-12
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