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2012 Fiscal Year Final Research Report

Regulatory mechanism of mouse GSC/niche system and in vitro spermatogenesis

Planned Research

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Project AreaRegulatory Mechanism of Gamete Stem Cells
Project/Area Number 20116005
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionYokohama City University

Principal Investigator

OGAWA Takehiko  横浜市立大学, 医学研究科, 准教授 (50254222)

Co-Investigator(Kenkyū-buntansha) OHBO Kazuyuki  横浜市立大学, 医学研究科, 教授 (70250751)
Project Period (FY) 2008 – 2012
Keywords精子形成 / 精子幹細胞 / 器官培養
Research Abstract

We succeeded to produce mouse sperm from spermatogonial stem cells (SSCs) in vitro. We adopted an organ culture method, and found that serum replacement was effective to induce complete spermatogenesis in our system. When cultured SSCs were transplanted into the seminiferous tubules of extracted host testes, they also differentiated into sperm in the cultured testis tissues. In addition, we demonstrated that an infertile model mouse, Sl/Sl^d mutant, can be treated with the explantation of their testis tissues.

  • Research Products

    (54 results)

All 2013 2012 2011 2010 2009 Other

All Journal Article (11 results) (of which Peer Reviewed: 10 results) Presentation (42 results) Remarks (1 results)

  • [Journal Article] In vitro spermatogenesis using an organ culture technique2013

    • Author(s)
      T. Yokonishi, T. Sato, K. Katagiri, T. Ogawa
    • Journal Title

      Methods Mol Biol

      Volume: 927 Pages: 479-488

  • [Journal Article] Testis tissue explantation cures spermatogenic failure in c-Kit ligand mutant mice.2012

    • Author(s)
      T. Sato, T. Yokonishi, M. Komeya, K. Katagiri, Y. Kubota, S. Matoba, N. Ogonuki, A. Ogura, S. Yoshida, T. Ogawa
    • Journal Title

      Proc Natl Acad Sci USA

      Volume: 109 Pages: 16934-16938

    • DOI

      DOI:10.1073/pnas.1211845109

    • Peer Reviewed
  • [Journal Article] Splice variant HE4-V3 expression is associated with favorable prognosis in pulmonary adenocarcinoma2012

    • Author(s)
      K. Tokuishi, SI. Yamashita, K. Ohbo and K. Kawahara
    • Journal Title

      Tumour Biol

      Volume: 33 Pages: 103-109

    • DOI

      doi:10.1007/s13277-011-0252-8

    • Peer Reviewed
  • [Journal Article] Serum level of HE4 is closely associated with pulmonary adenocarcinoma progression2012

    • Author(s)
      SI. Yamashita, K. Tokuishi, T. Moroga, S. Yamamoto, K. Ohbo, S. Miyahara, Y. Yoshida, J. Yanagisawa, D. Hamatake, M. Hiratsuka, Y. Yoshinaga, T. Shiraishi, A. Iwasaki and K. Kawahara
    • Journal Title

      Tumour Biol

      Volume: 33 Pages: 2365-2370

    • DOI

      doi:10.1007/s13277-012-0499-8

    • Peer Reviewed
  • [Journal Article] In vitro production of fertile sperm from murine spermatogonial stem cell lines2011

    • Author(s)
      T. Sato, K. Katagiri, T. Yokonishi, Y. Kubota, K. Inoue, N. Ogonuki, S. Matoba, A. Ogura, T. Ogawa
    • Journal Title

      Nat Commun

      Volume: 2 Pages: 472

    • DOI

      doi:10.1038/ncomms1478

    • Peer Reviewed
  • [Journal Article] In vitro production of functional sperm in cultured neonatal mouse testes2011

    • Author(s)
      T. Sato, K. Katagiri, A. Gohbara, K. Inoue, N. Ogonuki, A. Ogura, Y. Kubota, T. Ogawa
    • Journal Title

      Nature

      Volume: 471 Pages: 504-507

    • DOI

      doi:10.1038/nature09850

    • Peer Reviewed
  • [Journal Article] HP1g links histone methylation marks to meiotic synapsis in mice2011

    • Author(s)
      Y. Takada Y, C. Naruse, Y. Costa, T. Shirakawa, M. Tachibana, J. Sharif, F. Kezuka-Shiotani, D. Kakiuchi, H. Masumoto, Y. Shinkai, K. Ohbo, A. H.F.M. Peters, J. A.M. Turner, M. Asano and K. Koseki
    • Journal Title

      Development

      Volume: 138 Pages: 4207-4217

    • DOI

      doi:10.1242/dev.064444

    • Peer Reviewed
  • [Journal Article] Proliferation of Mouse Spermatogonial Stem Cells in Microdrop Culture2010

    • Author(s)
      Y. Araki, T. Sato, K. Katagiri, Y. Kubota, Y. Araki, T. Ogawa
    • Journal Title

      Biol Reprod

      Volume: 83 Pages: 951-957

    • DOI

      doi:10.1095/ biolreprod.109.082800

    • Peer Reviewed
  • [Journal Article] In Vitro Murine Spermatogenesis in an Organ Culture System2010

    • Author(s)
      A. Gohbara, K. Katagiri, T. Sato, Y. Kubota, H. Kagechika, Y. Araki, Y. Araki, T. Ogawa
    • Journal Title

      Biol Reprod

      Volume: 83 Pages: 261-267

    • DOI

      doi:10.1095/biolreprod.110.083899

    • Peer Reviewed
  • [Journal Article] Ectopic porcine spermatogenesis in murine subcutis: tissue grafting versus cell-injection methods2009

    • Author(s)
      T. Watanabe, H. Hayashi, K. Kita, Y. Kubota, T. Ogawa
    • Journal Title

      Asian J Androl

      Volume: 11 Pages: 317-323

    • Peer Reviewed
  • [Journal Article] The histone3 lysine 4 methyltransferase, Mll2, is only required briefly in development and spermatogenesis2009

    • Author(s)
      Glaser S., Lubitz S., Loveland KL., Ohbo K., Robb L., Schwenk F., Seibler J., Roellig D, Kranz A., Anastassiadis K. and Stewart AF
    • Journal Title

      Epigenetics & Chromatin

      Volume: 2 Pages: 5

    • DOI

      doi:10.1186/1756-8935-2-5

    • Peer Reviewed
  • [Presentation] In vitro spermatogenesis using an organ culture method2013

    • Author(s)
      T. Ogawa
    • Organizer
      Australia Melbourne 10th International Congress of Andrology 10th International Congress of Andrology
    • Place of Presentation
      Melbourne, Australia
    • Year and Date
      20130223-26
  • [Presentation] 精子形成研究の変遷と展望2013

    • Author(s)
      小川毅彦
    • Organizer
      第101回日本泌尿器科学会総会
    • Place of Presentation
      さっぽろ芸術文化の館 (北海道)
    • Year and Date
      2013-04-28
  • [Presentation] 無精子症治療の新たな展開 - ヒト精細胞培養法の開発2013

    • Author(s)
      小川毅彦
    • Organizer
      第101回日本泌尿器科学会総会 シンポジウム5 新しい治療戦略シリーズ 男性不妊症診療の新たな戦略
    • Place of Presentation
      ロイトン札幌 (北海道)
    • Year and Date
      2013-04-28
  • [Presentation] In vitro reconstruction of functional mouse seminiferous tubules supporting germ cell differentiation 培養下での精巣組織再構成の試み2013

    • Author(s)
      T. Ogawa
    • Organizer
      第90回 日本生理学会大会
    • Place of Presentation
      タワーホール船堀(東京)
    • Year and Date
      2013-03-29
  • [Presentation] In vitro reconstruction of functional testicular tissues supporting spermatogenesis2013

    • Author(s)
      T. Ogawa
    • Organizer
      日本再生医療学会 Johnson & Johnson Innovation Award
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2013-03-22
  • [Presentation] In vitro spermatogenesis using an organ culture method2012

    • Author(s)
      T. Ogawa
    • Organizer
      Meeting GDRI "Mammalian Meiosis Network"
    • Place of Presentation
      La Maison du Seminaire, Nice, France
    • Year and Date
      20121011-12
  • [Presentation] 精巣組織培養法は無精子症を治療できるかシンポジウム2「無精子症の取り扱いに関する問題と展望」2012

    • Author(s)
      小川毅彦
    • Organizer
      日本生殖医学会学術講演会
    • Place of Presentation
      長崎ブリックホール(長崎県)
    • Year and Date
      2012-11-08
  • [Presentation] Induction of spermatogenesis in testis of c-Kit ligand mutant spermatogenic failure mice using Organ Culture method2012

    • Author(s)
      T.Ogawa
    • Organizer
      63rd Congress of Korean Society for Reproductive Medicine
    • Place of Presentation
      Seoul Women's University(Korea)
    • Year and Date
      2012-11-01
  • [Presentation] 精子幹細胞からの培養下精子形成2012

    • Author(s)
      小川毅彦
    • Organizer
      日本受精着床学会シンポジウム「基礎(1)精子幹細胞のバイオロジーとその応用」
    • Place of Presentation
      大阪国際会議場(大阪府)
    • Year and Date
      2012-08-30
  • [Presentation] Changing epigenetic modification and chromocenter formation associating with spermatogonia differentiation2012

    • Author(s)
      K. Ohbo, T. Shirakawa and H. Koseki
    • Organizer
      10^<th> .EMBL Conference, Transcription and Chromatin
    • Place of Presentation
      Heidelbeerg, Germany
    • Year and Date
      2012-08-26
  • [Presentation] In vitro spermatogenesis using an organ culture method2012

    • Author(s)
      T. Ogawa
    • Organizer
      The 58/60th NIBB symposium
    • Place of Presentation
      Okazaki, (Aichi prefecture) Japan
    • Year and Date
      2012-07-21
  • [Presentation] The 58th/60th NIBB Conference, Germline Specification, Sex, and Stem Cells2012

    • Author(s)
      K. Ohbo
    • Organizer
      Analysis of epigenetic changes associated with spermatogonia stem cell differentiation
    • Place of Presentation
      Okazaki, (Aichi prefecture) Japan
    • Year and Date
      2012-07-19
  • [Presentation] State-of-the-Art Lecture 1 "Testicular tissue culturing inducing complete spermatogenesis"2012

    • Author(s)
      T. Ogawa
    • Organizer
      The 7th AUA/JUA international affiliate society meeting
    • Place of Presentation
      Georgia World Congress Center, Atlanta
    • Year and Date
      2012-05-20
  • [Presentation] In vitro精子形成法の開発と不妊症診療への応用の可能性2012

    • Author(s)
      小川毅彦
    • Organizer
      第100回 日本泌尿器科学会総会SY2-5「男性不妊症に対する新たな挑戦」
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2012-04-22
  • [Presentation] 器官培養法を用いたin vitro精子形成法の開発2012

    • Author(s)
      小川毅彦
    • Organizer
      第20回平成不妊研究会
    • Place of Presentation
      六本木・アカデミーヒルズ(東京都)
    • Year and Date
      2012-03-15
  • [Presentation] Analysis of epigenetic feature of spermatogonial stem cells in mice2012

    • Author(s)
      K. Ohbo, T. Shirakawa, R. Yaman- Deveci, Y. Kamizato, K. Nakajima, H. Sone, Y. Sato, S. Jafar, Y. Takada-Horisawa, S. Yoshida, K. Ura, M. Muto and H. Koseki
    • Organizer
      Keystone Symposia, Epigenomics, Chromatin Dynamics
    • Place of Presentation
      Colorado, USA
    • Year and Date
      2012-01-19
  • [Presentation] 器官培養法を用いたin vitro精子形成法の開発2011

    • Author(s)
      小川毅彦
    • Organizer
      第26回動物生殖工学研究会
    • Place of Presentation
      北里大学・北里本館2F 大会議室(東京都)
    • Year and Date
      2011-12-04
  • [Presentation] In vitro精子形成法の開発と臨床応用の可能性2011

    • Author(s)
      小川毅彦
    • Organizer
      第3回生殖補助医療胚培養士セミナー
    • Place of Presentation
      JA共済ビル(東京都)
    • Year and Date
      2011-11-13
  • [Presentation] 器官培養法を用いた精子形成法の開発と臨床応用の可能性2011

    • Author(s)
      小川毅彦
    • Organizer
      第2回生殖医療研究会
    • Place of Presentation
      国際医療福祉大学 東京青山キャンパス(東京都)
    • Year and Date
      2011-11-06
  • [Presentation] 器官培養法を用いたin vitro精子形成法の開発と発展2011

    • Author(s)
      小川毅彦
    • Organizer
      第89回発生工学・疾患モデル研究会
    • Place of Presentation
      順天堂大学10号館8F803(東京都)
    • Year and Date
      2011-10-26
  • [Presentation] 器官培養法によるin vitro精子形成と臨床応用の可能性2011

    • Author(s)
      小川毅彦
    • Organizer
      第76回日本泌尿器科学会東部総会 シンポジウム
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2011-10-22
  • [Presentation] In vitro spermatogenesis2011

    • Author(s)
      T. Ogawa
    • Organizer
      57th Annual meeting of Canadian Fertility and Andrology Society
    • Place of Presentation
      Toronto, Canada
    • Year and Date
      2011-09-21
  • [Presentation] マウス精巣の器官培養によるin vitro精子形成2011

    • Author(s)
      小川毅彦
    • Organizer
      第104回日本繁殖学会シンポジウム「精原幹細胞:基礎から応用まで」
    • Place of Presentation
      いわて県民情報交流センター・アイーナ(岩手県)
    • Year and Date
      2011-09-17
  • [Presentation] In vitro production of functional sperm in cultured neonatal mouse testes2011

    • Author(s)
      T. Ogawa
    • Organizer
      16th World Congress on In vitro Fertilization & 5th World Congress on In vitro Maturation
    • Place of Presentation
      Keio Plaza (Tokyo), Japan
    • Year and Date
      2011-09-13
  • [Presentation] Identification of an epigenetic checkpoint that controls the differentiation from spermatogonial stem cells to progenitor cells2011

    • Author(s)
      T. Shirakawa, R. Yaman-Deveci, Y. Kamizato, K. Nakajima, H. Sone, Y. Sato, S. Jafar, Y. Takada-Horisawa, S. Yoshida, K. Ura, M. Muto, H. Koseki, T. Suda and K. Ohbo
    • Organizer
      Stem Cells in Development and Disease
    • Place of Presentation
      Berlin, Germany
    • Year and Date
      2011-09-12
  • [Presentation] An epigenetic checkpoint controls the transition from a stem cell pool to a progenitor cell stage in testes2011

    • Author(s)
      K. Ohbo
    • Organizer
      EMBO Conference Series on Chromatin and Epigenetics
    • Place of Presentation
      EMBL, Heidelberg, Germany
    • Year and Date
      2011-06-04
  • [Presentation] 器官培養法を用いた新生仔マウス精巣でのin vitro精子産生2011

    • Author(s)
      小川毅彦、佐藤卓也、横西哲広、郷原絢子、河路かおる、窪田吉信
    • Organizer
      第99回 日本泌尿器科学会総会
    • Place of Presentation
      名古屋国際会議場(愛知県)
    • Year and Date
      2011-04-22
  • [Presentation] in vitro精子形成の進展2011

    • Author(s)
      小川毅彦
    • Organizer
      第6回 日本生殖再生医学会
    • Place of Presentation
      シェーンバッハ・サボー(東京都)
    • Year and Date
      2011-03-13
  • [Presentation] 精子幹細胞の培養とin vitro 精子形成の進展2010

    • Author(s)
      小川毅彦
    • Organizer
      第55回日本生殖医学会総会/シンポジウム3「精子形成研究のあらたなるフロンティア: 幹細胞から精子の品質まで」
    • Place of Presentation
      徳島市あわぎんホール(徳島県)
    • Year and Date
      2010-11-12
  • [Presentation] In vitro production of functional sperm in cultured neonatal mouse testes2010

    • Author(s)
      T. Ogawa, T. Sato, K. Katagiri, Y. Kubota
    • Organizer
      Cold Spring Harbor Symposium on Germ Cells
    • Place of Presentation
      NewYork, USA
    • Year and Date
      2010-10-08
  • [Presentation] Epigenetic difference between stem cells and progenitor cells in testes2010

    • Author(s)
      K. Ohbo
    • Organizer
      Advances in Epigenetics Epigenome NoE and Chromatin Plasticity
    • Place of Presentation
      San Feliu deGuixols, France
    • Year and Date
      2010-05-13
  • [Presentation] エピジェネティクスの視点から見た、精巣幹細胞維持と前駆細胞への分化メカニズム2010

    • Author(s)
      大保和之,白川峰征、中島久仁子
    • Organizer
      第115回日本解剖学会総会
    • Place of Presentation
      盛岡・岩手県民会館(岩手県)
    • Year and Date
      2010-03-30
  • [Presentation] 培養精子幹細胞(GS細胞)への効率的な遺伝子導入2010

    • Author(s)
      佐藤卓也、片桐久美子、窪田吉信、小川毅彦
    • Organizer
      第5回日本生殖再生医学会学術集会
    • Place of Presentation
      シェーンバッハ・サボー(東京都)
    • Year and Date
      2010-02-21
  • [Presentation] 精原幹細胞と精子形成2010

    • Author(s)
      小川毅彦、佐藤卓也 、片桐久美子、窪田吉信、荒木泰行、荒木康久
    • Organizer
      第13回 RMB研究会シンポジウム
    • Place of Presentation
      持田製薬株式会社「ルークホール」(東京都)
    • Year and Date
      2010-01-23
  • [Presentation] マウス精子幹細胞のマイクロドロップ培養下での増殖動態2009

    • Author(s)
      小川毅彦、荒木泰行、荒木康久
    • Organizer
      第41回精子研究会
    • Place of Presentation
      東京大学 駒場キャンパス 16号館 119-129室(東京都)
    • Year and Date
      2009-12-19
  • [Presentation] 精子幹細胞の培養と精子形成2009

    • Author(s)
      小川毅彦
    • Organizer
      第14回日本生殖内分泌学会学術集会 シンポジウム-生殖臓器由来幹細胞研究の最前線
    • Place of Presentation
      シェーンバッハ・サボー(東京都)
    • Year and Date
      2009-11-28
  • [Presentation] Difference of epigenetic modifications between self-renewing tissue-specific stem cells and progenitor cells in mouse testes2009

    • Author(s)
      Shikarawa T, Ura K, Yoshida S, Ohbo K
    • Organizer
      EMBO Conference Series on Nuclear Structure and Dynamics
    • Place of Presentation
      Isle sur la Sorgue, France
    • Year and Date
      2009-10-03
  • [Presentation] in vitro にもニッチはあるか?2009

    • Author(s)
      小川毅彦
    • Organizer
      第80回 日本動物学会・シンポジウム「配偶子幹細胞制御に関する研究の新展開」
    • Place of Presentation
      静岡グランシップ(静岡県)
    • Year and Date
      2009-09-17
  • [Presentation] Dynamic epigenetic changes in differentiation step from spermatogonial stem cells to progenitor cells2009

    • Author(s)
      Shirakawa T, Ohbo K
    • Organizer
      Gordon Research Conferences Mammalian Gametogenesis & Embyogenesis
    • Place of Presentation
      Waterville Valley, NH, USA
    • Year and Date
      2009-08-03
  • [Presentation] In vitro murine spermatogenesis in an organ culture system2009

    • Author(s)
      T. Ogawa, K. Katagiri, Y. Araki, Y. Araki, Y. Kubota
    • Organizer
      European Society of Human Reproduction and Embryology (ESHRE)
    • Place of Presentation
      Amsterdam, Nederland
    • Year and Date
      2009-07-01
  • [Presentation] 精巣幹細胞特異的発現分子により可視化された幹細胞のゲノム修飾動態2009

    • Author(s)
      大保 和之
    • Organizer
      シンポジウム「生殖系細胞の機能発現とイメージング」第114回日本解剖学会総会・全国学術総会
    • Place of Presentation
      岡山理科大学(岡山県)
    • Year and Date
      2009-03-28
  • [Presentation] 精巣幹細胞から前駆細胞への分化に伴う遺伝子発現制御機構の変化2009

    • Author(s)
      大保和之
    • Organizer
      SSRE生殖工学研究会シンポジウム「生殖幹細胞の分化制御機構」
    • Place of Presentation
      明治大学駿河台校舎(東京都)
    • Year and Date
      2009-03-07
  • [Remarks]

    • URL

      http://www-user.yokohama-cu.ac.jp/~urology/

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Published: 2014-08-29  

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