2012 Fiscal Year Final Research Report
Identification of GSCs and their niche cells and their seasonal regulation in salmonids
| Project Area | Regulatory Mechanism of Gamete Stem Cells |
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| Project/Area Number |
20116006
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| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Tokyo University of Marine Science and Technology |
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Principal Investigator |
YOSHIZAKI Goro 東京海洋大学, 海洋科学技術研究科, 教授 (70281003)
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| Project Period (FY) |
2008 – 2012
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| Keywords | 生殖幹細胞 / ニジマス / 生殖細胞移植 |
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| Research Abstract |
1）We reveled that rainbow trout ovary possessed oogonial populations which can behave as GSCs and carry high sexual plasticity. 2) We revealed that undifferentiated spermatogonia located in post-spermiated testes showed clear seasonal patterns for their transplantability (stemness). Further, by comparing the transcripts between spermatogonia with and without transplantability, we found several molecular markers that can be used as indicators for their stemness. 3) We found GSCs are highly enriched in side population after staining testicular cells with Hoechst 33342. We also characterize the side population by transcriptomical and morphological methods.
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