ROS-sensitive protein phosphatase and its signal transduction

2010 Fiscal Year Self-evaluation Report

• Project Area: Signaling functions of reactive oxygen species
• Project/Area Number: 20117012
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Hiroshima University
• Principal Investigator: KAMATA Hideaki Hiroshima University, 大学院・医歯薬学総合研究科, 准教授 (10233925)
• Project Period (FY): 2008-11-13 – 2013-03-31
• Keywords: シグナル伝達 / 活性酸素 / キナーゼ / ホスファターゼ / ユビキチン / プロテアソーム / NF-kB

Research Abstract

本研究は活性酸素(ROS)のシグナル機能の解明を目的に、細胞制御における活性酸素応答ホスファターゼの機能と役割と、ROSの産生制御機構の解明をおこなう。具体的には、(1)腫瘍壊死因子(TNFα)によるROSの産生機構、(2)活性酸素応答ホスファターゼによる活性酸素シグナルからリン酸化シグナルへの変換機構、(3)ROSによるNF-κBと活性酸素応答ホスファターゼを介したタンパク質分解・遺伝子発現系の制御機構、(4)活性酸素シグナルの制御異常と疾患との連関について解析し、炎症や発癌における活性酸素シグナルの役割を解明する。

Research Products

• [Journal Article] Peptidyl-prolyl cis/trans isomerase NIMA- interacting 1 associates with IRS-1 and enhances insulin actions and adipogenesis (2011)
  • Author(s): Yusuke Nakatsu, Hideyuki Sakoda, Akifumi Kushiyama, Jun Zhang, Hiraku Ono, Midori Fujishiro, Takako Kikuchi, Toshiaki Fukushima, Masayasu Yoneda, Haruya Ohno, Nanao Horike, Machi Kanna, Yoshihiro Tsuchiya, Hideaki Kamata, Fusanori Nishimura, Toshiak Isobe, Takehide Ogihara, Hideki Katagiri, Yoshitomo Oka, Shin-ichiro Takahashi, Hiroki Kurihara, Takafumi Uchida, Tomoichiro Asano
  • Journal Title: Journal of Biological Chemistry 286 Pages: 20812-20822
  • Peer Reviewed
• [Journal Article] 4F2hc stabilizes GLUT1 protein and increases glucose transport activity.American Journal of Physiology (2011)
  • Author(s): Haruya 0hno, Yusuke Nakatsu, Hideyuki Sakoda, Akifumi Kushiyama, Hiraku Ono, Midori Fujishiro, Yuichiro Otani, Hirofumi Okubo, Masayasu Yoneda, Toshiaki Fukushima, Yoshihiro Tsuchiya, Hideaki Kamata, Fusanori Nishimura, Hiroki Kurihara, Hideki Katagiri, Yoshitomo Oka, Tomoichiro Asano
  • Journal Title: Cell Physiolog 300 Pages: 1047-1054
  • Peer Reviewed
• [Journal Article] IKKβ is an adaptor protein for β-TrCP mediated IKBα ubiquitination in UV-induced NF-κB activation (2010)
  • Author(s): Yoshihiro Tsuchiya, Tomoichiro Asano, Keiko Nakayama, Tomohisa Kato Jr., Michael Karin, Hideaki Kamata
  • Journal Title: Molecular Cell 39 Pages: 570-582
  • Peer Reviewed
• [Journal Article] IκBβ is a positive and negative regulator of NF-κB activity during inflammation (2010)
  • Author(s): Hideaki Kamata, Yoshihiro Tsuchiya, Tomoichiro Asano
  • Journal Title: Cell Research 20 Pages: 1178-1180
  • Peer Reviewed
• [Journal Article] Pinl associates with and induces translocation of CRTC2 to the cytosol, thereby suppressing CRE transcriptional activity (2010)
  • Author(s): Yusuke Nakatsu, Hideyuki Sakoda, Akifumi Kushiyama, Hiraku Ono, Midori Fujishiro, Nanao Horike, Masayasu Yoneda, Haruya Ohno, Yoshihiro Tsuchiya, Hideaki Kamata, Hidetoshi Tahara, Toshiaki Isobe, Fusanori Nishimura, Hideki Katagiri, Yoshitomo Oka, Toshiaki Fukushima, Shin-Ichiro, Takahashi, Hiroki Kurihara, Takafumi Uchida, Tomoichiro Asano
  • Journal Title: Journal of Biological Chemistry 285 Pages: 33018-33027
  • Peer Reviewed
• [Presentation] IKKβ act as an adaptor protein for IκBα ubiquitination and degradation in UV-induced NF-κB activation possible involvement in the TNF signaling (2011)
  • Author(s): Yoshihiro Tsuchiya , Hideaki Kamata
  • Organizer: 13th International TNF Conference, Awajishima
  • Place of Presentation: Japan
  • Year and Date: 2011-05-17
• [Presentation] NF-κBと活性酸素(ROS)シグナルに連関したタンパク質のリン酸化と分解による細胞応答 (2010)
  • Author(s): 鎌田英明, 土谷佳弘, 浅野知一郎
  • Organizer: 第33回日本分子生物学会・第83回日本生化学会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-09
• [Presentation] 細胞内ストレスに応答した核内IKKRを介した新規NF-KB活性化機構 (2010)
  • Author(s): 土谷佳弘, 浅野知一郎, 鎌田英明
  • Organizer: 第33回日本分子生物学会・第83回日本生化学会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-09
• [Presentation] Nuclear IKKb acts as an adaptor protein for IκBα degradation in UV-induced NF-KB activation (2010)
  • Author(s): 鎌田英明, 土谷佳弘
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: シンポジウム, 大阪
  • Year and Date: 2010-09-24