Generation of genetically modified primates using cultured germ cells

2022 Fiscal Year Final Research Report

• Project Area: A new foundation for primate developmental biology
• Project/Area Number: 20H05764
• Research Category: Grant-in-Aid for Transformative Research Areas (B)
• Allocation Type: Single-year Grants
• Review Section: Transformative Research Areas, Section (III)
• Research Institution: National Center for Child Health and Development (2022); Central Institute for Experimental Animals (2020-2021)
• Principal Investigator: Watanabe Toshiaki 国立研究開発法人国立成育医療研究センター, 細胞医療研究部, 専門職 (40715405)
• Project Period (FY): 2020-10-02 – 2023-03-31
• Keywords: マーモセット / 生殖細胞 / 始原生殖細胞様細胞 / mRNA transfection / iPS細胞 / 分化誘導

Outline of Final Research Achievements

I developed two methods for putting forward the development of PGCLCs. The First method involves the generation of xenogenic reconstituted testes under the kidney capsule of immunodeficient mice. In the reconstituted testes, marmoset PGCLCs are differentiated into late PGCs 30 days after the transplantation, and then they are further differentiated into gonocytes in 90 days. The second method is auto-transplantation of marmoset PGCLCs and marmoset newborn testes cells under the kidney capsule of marmosets to form the reconstituted testes. In the reconstituted testes, PGCLCs similarly differentiated into early gonocytes. We are now working on long term experiment to achieve further development.

Free Research Field

生殖生物学

Academic Significance and Societal Importance of the Research Achievements

本研究で開発した技術は、細胞ベースの遺伝子改変マーモセット作製技術を確立していくための基盤となる。また、マーモセット（霊長類）の初期生殖細胞発生系を研究するための実験系として役立つ。