2013 Fiscal Year Final Research Report
Characterization of structural dynamics of intrinsically disordered proteins
| Project Area | Target recognition and expression mechanism of intrinsically disordered protein |
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| Project/Area Number |
21113003
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| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Yokohama City University |
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Principal Investigator |
AKASHI Satoko 横浜市立大学, その他の研究科, 准教授 (10280728)
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| Co-Investigator(Kenkyū-buntansha) |
SUGASE Kenji 公益財団法人 サントリー生命科学財団 生物有機科学研究所, 主席研究員 (00300822)
MIZUGUCHI Mineyuki 富山大学, 大学院医学薬学研究部, 教授 (30332662)
NISHIMURA Yoshifumi 横浜市立大学, 大学院生命医科学研究科, 教授 (70107390)
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| Project Period (FY) |
2009-07-23 – 2014-03-31
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| Keywords | 蛋白質 / 生体分子 / NMR / 質量分析 / 天然変性 |
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| Research Abstract |
In order to understand functionally important intrinsically disordered proteins (IDPs) from their structure and to clarify their target recognition mechanisms, characterization of dynamic structures of IDPs was carried out using NMR and mass spectrometry (MS). The project achievements are (1) Analysis of gas-phase behaviors of the histone multimers and homologous recombination related factor Swi5-Sfr1 complexes, (2) NMR structural analysis of iPS cell-inducing transcription factors Sox2 and Oct3/4, (3) Structure determination of the complex of splicing factor PQBP1and spliceosomal protein U5-15kD and PQBP1-U5-15kD-U5-52K, (4) Identification of two possible inhibitors of NRSF-Sin3 interaction based on the NMR complex structure, (5) Development of a fast and accurate fitting program of NMR relaxation dispersion data, and (6) NMR analysis of the complex of chromodomain of HP1 and an H3K9me peptide.
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[Journal Article] Mutations in the PQBP1 gene prevent its interaction with the spliceosomal protein U5-15kD2014
Author(s)
Mizuguchi, M., Obita, T., Serita, T., Kojima, R., Nabeshima, Y., Okazawa, H.
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Journal Title
Nat. Commun
Volume: 5
Pages: 3822
DOI
Peer Reviewed
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[Journal Article] Quantitative analysis of location- and sequence-dependent deamination by APOBEC3G using real-time NMR spectroscopy2014
Author(s)
Furukawa, A., Sugase, K., Morishita, R., Nagata, T., Kodaki, T., Takaori-Kondo, A., Ryo, A., Katahira, M.
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Journal Title
Angew. Chem. Int. Ed. Engl.
Volume: 53
Pages: 2349-2352
DOI
Peer Reviewed
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[Journal Article] Conclusive evidence of the reconstituted hexasome proved by native mass spectrometry2013
Author(s)
Azegami, N., Saikusa, K., Todokoro, Y., Kurumizaka, H., Nagadoi, A, Nishimura, Y, Akashi, S.
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Journal Title
Biochemistry
Volume: 52
Pages: 5155-5157
DOI
Peer Reviewed
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[Journal Article] Gas-phase structure of the histone multimers characterized by ion mobility mass spectrometry and molecular dynamics simulation2013
Author(s)
Saikusa, K., Fuchigami, S., Takahashi, K., Asano, Y., Nagadoi, A., Tachiwana, H., Kurumizaka, H., Ikeguchi, M., Nishimura, Y., Akashi, S.
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Journal Title
Anal. Chem.
Volume: 85
Pages: 4165-4171
DOI
Peer Reviewed
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[Journal Article] Characterisation of an intrinsically disordered protein complex of Swi5-Sfr1 by ion mobility mass spectrometry and small-angle X-ray scattering2013
Author(s)
Saikusa, K., Kuwabara, N., Kokabu, Y., Inoue, Y., Sato, M., Iwasaki, H., Shimizu, T., Ikeguchi, M., Akashi, S.
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Journal Title
Analyst
Volume: 138
Pages: 1441-1449
DOI
Peer Reviewed
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[Journal Article] Structural characterization of a trapped folding intermediate of pyrrolidone carboxyl peptidase from a hyperthermophile2012
Author(s)
Mizuguchi, M., Takeuchi, M., Ohki, S., Nabeshima, Y., Kouno, T., Aizawa, T., Demura, M., Kawano, K., Yutani, K.
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Journal Title
Biochemistry
Volume: 51
Pages: 6089-6096
DOI
Peer Reviewed
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