2013 Fiscal Year Final Research Report
Analysis of exracellular signal in cell community of early embryos
| Project Area | Cell Community in early mammalian development |
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| Project/Area Number |
21116002
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| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Kyushu University |
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Principal Investigator |
MENO Chikara 九州大学, 医学(系)研究科(研究院), 教授 (20311764)
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| Co-Investigator(Kenkyū-buntansha) |
WADA Yoh 大阪大学, 産業科学研究所, 准教授 (50212329)
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| Co-Investigator(Renkei-kenkyūsha) |
KITAJIMA Keiko 九州大学, 医学研究院, 助教 (00332784)
OKI Shinya 九州大学, 医学研究院, 助教 (90452713)
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| Project Period (FY) |
2009-07-23 – 2014-03-31
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| Keywords | 細胞外シグナル / 左右軸形成 / 原腸胚形成 / 多能性幹細胞 / エンドサイトーシス / 小胞輸送 |
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| Research Abstract |
The epiblast in mouse embryos acquires proximo-distal (P-D) and antero-posterior (A-P) axes by interacting with the extra-embryonic ectoderm and visceral endoderm (VE). In the VE, down-regulation of BMP signaling required for the P-D specification occurs via mVam2-dependent microautophagy. After establishment of the A-P axis, Wnt3 in the posterior epiblast drives the primitive streak formation. We showed that epiblast stem cells (EpiSCs), which often differentiate into the mesendoderm, could be maintained by suppressing Wnt canonical pathway, showing that the Wnt signaling promotes differentiation of EpiSCs into mesendoderm. During the left-right (L-R) specification, Wnt3 was found to be expressed asymmetrically along the L-R axis in the node. We identified the molecular network involved in the asymmetric expression thereby revealed the canonical Wnt signaling as a novel mechanism for the L-R axis determination.
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