Construction of a New Catalyst Using a Protein Matrix

2015 Fiscal Year Final Research Report

• Project Area: Molecule Activation Directed toward Straightforward Synthesis
• Project/Area Number: 22105013
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Science and Engineering
• Research Institution: Osaka University
• Principal Investigator: Hayashi Takashi 大阪大学, 工学(系)研究科(研究院), 教授 (20222226)
• Co-Investigator(Renkei-kenkyūsha): ONODA Akira 大阪大学, 大学院工学研究科, 准教授 (60366424)
• Project Period (FY): 2010-04-01 – 2016-03-31
• Keywords: タンパク質 / 反応場 / 物質変換 / 生体触媒 / 金属酵素

Outline of Final Research Achievements

Biosynthesis and metabolic degradation are one of the fundamental examples which are related to the straight toward organic synthesis. The efficient reactions require enzymes as a biocatalyst. Therefore, it will be an appropriate approach for chemists to modify a protein to yield a useful catalyst. Our group has focused on the construction of a biohybrid catalyst in which an artificially created metal complex is linked into an aproprotein after the removal of a cofactor heme of a hemoprotein. As a result, we have demonstrated that several biohybrid catalysts can promote reactions such as C-H bond activation, C-C bond formation or hydrogen evolution which seems to be difficult for natural enzymes. Furthermore, we indicate that the fine-tuning of the reaction scaffold of our biohybrid catalyst by mutagenetic approach is capable of controlling the reaction activity and stereoselectivity.

Free Research Field

生物無機化学