2014 Fiscal Year Final Research Report
Versatile host for the heterologous expression of secondary metabolism.
Project Area | Biosynthetic machinery: Deciphering and regulating the system for bioactive metabolite diversification |
Project/Area Number |
22108006
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Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
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Allocation Type | Single-year Grants |
Review Section |
Science and Engineering
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Research Institution | Kitasato University |
Principal Investigator |
IKEDA Haruo 北里大学, 感染制御科学府, 教授 (90159632)
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Co-Investigator(Kenkyū-buntansha) |
KOMATSU Mamoru 北里大学, 大学院感染制御科学府, 講師 (40414057)
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Project Period (FY) |
2010-04-01 – 2015-03-31
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Keywords | 二次代謝産物 / 生合成マシナリー / 異種発現 / 放線菌 |
Outline of Final Research Achievements |
An industrial microorganism Streptomyces avermitilis, which is a producer of anthelmintic macrocyclic lactones, avermectins, has been constructed as a versatile model host for heterologous expression of genes encoding secondary metabolite biosynthesis. Twenty of the entire biosynthetic gene clusters for secondary metabolites were successively cloned and introduced into a versatile model host S. avermitilis SUKA17 or 22. Almost all S. avermitilis transformants carrying the entire gene cluster produced metabolites as a result of the expression of biosynthetic gene clusters introduced. A few transformants were unable to produce metabolites but their production was restored by the expression of biosynthetic genes using an alternative promoter or the expression of a regulatory gene in the gene cluster that controls the expression of biosynthetic genes in the cluster using an alternative promoter.
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Free Research Field |
微生物分子遺伝学
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