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2014 Fiscal Year Final Research Report

Regulatory mechanisms of immune cell trafficking by spatiotemporal control of cell adhesion

Planned Research

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Project AreaCross-talk between moving cells and microenvironment as a basis of emerging order in multicellular systems
Project/Area Number 22111003
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionKansai Medical University

Principal Investigator

KINASHI Tatsuo  関西医科大学, 医学部, 教授 (30202039)

Co-Investigator(Renkei-kenkyūsha) KATAKAI Tomoya  新潟大学, 医学部, 教授 (00324682)
UEDA Yoshihiro  関西医科大学, 医学部, 講師 (90533208)
KONDO Naoyuki  関西医科大学, 医学部, 助教 (30570840)
KITA Toshiyuki  関西医科大学, 医学部, 助教 (70589986)
Project Period (FY) 2010-04-01 – 2015-03-31
Keywordsリンパ球 / ケモカイン / インテグリン / Rap1 / ストローマ細胞
Outline of Final Research Achievements

We examined lymphocyte intranodal migration and antigen recognition to clarify the the functions and regulations of LFA-1 mainly using two-photon imaging techniques. We demonstrate: (1) there are two migration modes in peripheral lymph nodes; chemokine-independent random migration and chemokine-dependent directional migration, the latter of which depends on LFA-1 binding to dendritic ICAM-1, (2) thymocytes exhibit fast migration within the medulla of the thymus, and recognize self-antigen, which require LFA-1/ICAM-1 dependent adhesion regulated by Mst1, (3) regulatory T cells show migratory immune synapses in vitro and in lymph nodes , which regulated by Mst1. We further establish single-molecule analysis of LFA-1/ICAM-1 bindings in immune synapse in which Rap1/Mst-1 regulate high-affinity bindings.

Free Research Field

免疫学

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Published: 2016-06-03  

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