2014 Fiscal Year Final Research Report
Regulatory mechanisms of immune cell trafficking by spatiotemporal control of cell adhesion
Project Area | Cross-talk between moving cells and microenvironment as a basis of emerging order in multicellular systems |
Project/Area Number |
22111003
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Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
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Allocation Type | Single-year Grants |
Review Section |
Biological Sciences
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Research Institution | Kansai Medical University |
Principal Investigator |
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Co-Investigator(Renkei-kenkyūsha) |
KATAKAI Tomoya 新潟大学, 医学部, 教授 (00324682)
UEDA Yoshihiro 関西医科大学, 医学部, 講師 (90533208)
KONDO Naoyuki 関西医科大学, 医学部, 助教 (30570840)
KITA Toshiyuki 関西医科大学, 医学部, 助教 (70589986)
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Project Period (FY) |
2010-04-01 – 2015-03-31
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Keywords | リンパ球 / ケモカイン / インテグリン / Rap1 / ストローマ細胞 |
Outline of Final Research Achievements |
We examined lymphocyte intranodal migration and antigen recognition to clarify the the functions and regulations of LFA-1 mainly using two-photon imaging techniques. We demonstrate: (1) there are two migration modes in peripheral lymph nodes; chemokine-independent random migration and chemokine-dependent directional migration, the latter of which depends on LFA-1 binding to dendritic ICAM-1, (2) thymocytes exhibit fast migration within the medulla of the thymus, and recognize self-antigen, which require LFA-1/ICAM-1 dependent adhesion regulated by Mst1, (3) regulatory T cells show migratory immune synapses in vitro and in lymph nodes , which regulated by Mst1. We further establish single-molecule analysis of LFA-1/ICAM-1 bindings in immune synapse in which Rap1/Mst-1 regulate high-affinity bindings.
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Free Research Field |
免疫学
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