Histone signaling network in DNA damage response

2014 Fiscal Year Final Research Report

• Project Area: Coupling of replication, repair and transcription, and their common mechanism of chromatin remodeling
• Project/Area Number: 22131004
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Kyoto University
• Principal Investigator: IKURA TSUYOSHI 京都大学, 放射線生物研究センター, 准教授 (70335686)
• Project Period (FY): 2010-04-01 – 2015-03-31
• Keywords: TIP60ヒストンアセチル化酵素 / ヒストンアセチル化 / ヒストン交換反応 / ヒストンH2AX / クロマチンダイナミクス / DNA損傷応答

Outline of Final Research Achievements

The eukaryotic genome is tightly packed into the chromatin, a hierarchically organized complex of DNA, histone and nonhistone proteins. Recent findings indicates that histone variant eviction/exchange has an important role in DNA repair and DNA damage response. We found that the TIP60 complex facilitates histone H2AX exchange at the sites of DNA damage. Interestingly, the phosphorylation of histone H2AX is not required for histone H2AX exchange. In this project, we investigated the physiological significance of histone H2AX exchange in DNA damage response. We found that the histone signaling network regulated by the acetylation-dependent histone H2AX exchange is cross-talked with the phosphorylation-dependent DNA damage response signaling.

Free Research Field

放射線生物学