Primary cilia and cell cycle

2017 Fiscal Year Final Research Report

• Project Area: Cilium-centrosome system regulating biosignal flows
• Project/Area Number: 24113005
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Mie University (2016-2017); Aichi Cancer Center Research Institute (2012-2015)
• Principal Investigator: Inagaki Masaki 三重大学, 医学系研究科, 教授 (30183007)
• Co-Investigator(Kenkyū-buntansha): 五島 直樹 国立研究開発法人産業技術総合研究所, 生命工学領域, 研究チーム長 (70215482)
• Co-Investigator(Renkei-kenkyūsha): IZAWA Ichiro 名古屋学芸大学, 管理栄養学部, 教授 (20311441)
• Project Period (FY): 2012-06-28 – 2017-03-31
• Keywords: 一次シリア / 中心体 / 細胞周期 / ユビキチン / プロテアソーム / 成長因子受容体

Outline of Final Research Achievements

In this study, we show that ubiquitin-proteasome system controls the assembly and disassembly of primary cilia: CRL3-KCTD17 ubiquitin E3 ligase leads to poly-ubiquitination and degradation of trichoplein, a suppressor of ciliogenesis, thereby inducing ciliogenesis in response to serum starvation. In proliferating cells, trichoplein is destabilized by USP8 deubiquitinase. Importantly, we reveal that epidermal growth factor receptor (EGFR) kinase directly phosphorylates and activates USP8. These data indicate that the ubiquitin-proteasome-mediated control of trichoplein level is critical for ciliogenesis.

Free Research Field

分子生理学