Elucidation of control mechanisms of transcriptional checkpoint during Pol II elongation, termination, and recycle

2016 Fiscal Year Final Research Report

• Project Area: Integral understanding of the mechanism of transcription cycle through quantitative, high-resolution approaches
• Project/Area Number: 24118002
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: YAMAGUCHI Yuki 東京工業大学, 生命理工学院, 教授 (50345360)
• Co-Investigator(Kenkyū-buntansha): 田村 智彦 横浜市立大学, 医学(系)研究科(研究院), 教授 (50285144) ; 高橋 秀尚 北海道大学, 医学(系)研究科(研究院), 講師 (30423544) ; 川内 潤也 東京医科歯科大学, 難治疾患研究所, 助教 (20544498)
• Co-Investigator(Renkei-kenkyūsha): NISHIYAMA Akira 横浜市立大学, 大学院医学研究科, 准教授 (80589664) ; NAKABAYASHI Jun 横浜市立大学, 先端医科学研究センター, 准教授 (80322733)
• Research Collaborator: ISOBE Tomoyasu ; YAMAMOTO Junichi ; KATO Junko ; SUZUKI Hidefumi ; SOU Seifuku ; SHIBATA Hirotaka ; TATENO Shumpei ; HASEGAWA Sakiko ; ARAKAKI Takayuki ; ZUKERAN Ari ; KUROTAKI Daisuke ; BAN Tatsuma ; FUSHIMI Kentaro
• Project Period (FY): 2012-06-28 – 2017-03-31
• Keywords: 遺伝子 / 発現制御 / 発生・分化 / 生体生命情報学 / 酵素

Outline of Final Research Achievements

Synthesis of full-length RNAs is made possible through multiple interactions of Pol II with DNA, RNA, and proteins (e.g., chromatin factors, transcription initiation factors, elongation factors, termination factors, and RNA processing factors), which together form a large complex that undergoes rapid remodeling during the transcription cycle. However, the detail of this dynamic process remains poorly understood. Here we set out to explore mechanisms for the remodeling of the Pol II-containing complex during the transcription cycle and regulatory checkpoints in it, through identification and characterization of new Pol II-interacting factors and kinetic analyses in living cells.

Free Research Field

分子生物学・生化学