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1991 Fiscal Year Final Research Report Summary

Studies on DNA damages in vivo and in vitro by highly sensitive fluorometric analysis

Research Project

Project/Area Number 01044096
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research InstitutionOkayama University

Principal Investigator

HAYATSU Hikoya  Faculty of Pharmaceutical Sciences, Okayama University, 薬学部, 教授 (10012593)

Co-Investigator(Kenkyū-buntansha) NEGISHI Tomoe  Faculty of Pharmaceutical Sciences, Okayama University, 薬学部, 非常勤講師 (80116491)
NEGISHI Kazuo  Gene Research Center, Okayama University, 遺伝子実験施設, 助教授 (70116490)
WATAYA Yusuke  Faculty of Pharmaceutical Sciences, Okayama University, 薬学部, 助教授 (90127598)
MORGAN A. R.  Department of Biochemistry, University of Alberta, Professor
Project Period (FY) 1989 – 1991
Keywordsfluorometry / DNA damage / N-nitrosamines / 8-methoxypsoralen / light / 近紫外光 / 突然変異
Research Abstract

DNA damages can be detected with high sensitivity by use of a fluorometric measurement. This method, originally developed by Morgan (the coinvestigator of this project), is based on the change in the fluorescence-intensity of ethidium bromide upon its intercalation into double-stranded DNA. Through our collaboration with Dr. Morgan, the following achievements were made.
1. The fluorometric technique has been acquired by the Japanese workers, and the fluorometric measurement system is now established in Okayama University.
2. Several new DNA-damaging agents were discovered. They include tetranitromethane, phosgene and N-nitrosamines with light.
3. Cross-linking was detected in DNA that had been treated in vitro with 8-methoxypsoralen and UVA, but no cross-linking was found in the DNA of Drosophila lavae that had been treated with these agents in vivo. These observations indicate that DNA damages other than cross-linking must be responsible for the mutagenesis observed in the wing spots of the adult flies obtained from the lavae.

  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] T.Negishi,H.Hayatsu et al.: "The genotoxicities in vivo of N-nitrosamines as assayed in the wing spot test and the DNA-repair test of Drosophila melanogaster" Mutation Research. 252. 119-128 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Tsuchiyama,H.Hayatsu et al.: "Analysis of 2-amino-N^6-hydroxyadenine-induced mutagenesis in phage M13mp2" Mutation Research. 253. 47-54 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A.Matsuda,H.Hayatsu et al.: "Mutagenicity of (p-nitrophenyl)adenines in Salmonella typhimurium." Mutation Research. 263. 93-100 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Hayatsu,et al.: "Suppression of genotoxicity of carcinogens by (-) epigallocatechin gallate." Preventive Medicine.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Hayatsu: "Dietary inhibitors of mutagenesis and carcinogenesis." Chemoprevention of Cancer,eds.by S.V.Bhide and G.B.Maru,Omega Scientific Publications,New Delhi. 40-50 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Aoe,H.Hayatsu et al.: "DNA single-strand breaks induced in vitro by N-nitrosamines with irradiation of near ultraviolet light." Mutation Research,.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Hayatsu (ed.): "Mutagens in Food:Detection and Prevention" CRC Press,FL, 1-286 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H. Tsuchiyama, H. Hayatsu et al.: "Analysis of 2-amino-N^6-hydroxyadenine-induced mutagenesis in phage M13mp2" Mutation Research. 253. 47-54 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A. Matsuda, H. Hayatsu et al.: "Mutagenicity of (p-nitrophenyl) adenines in Salmonella typhimurium" Mutation Research. 263. 93-100 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Hayatsu et al.: "Suppression of genotoxicity of carcinogens by (-) epidallocatechin gallate" Preventive Medicine.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Hayatsu: "Dietary inhibitors of mutagenesis and carcinogenesis" Chemoprevention of Cancer, eds. by S. V. Bhide and G. B. Maru, Omega Scientific Publications, New Delhi. 40-50 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Aoe, H. Hayatsu et al.: "DNA single-strand breaks induced in vitro by N-nitrosamines with irradiation of near ultraviolet light" Mutation Research.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Hayatsu (ed.): CRC Press, FL. Mutagens in Food : Detection and Prevention, 1-286 (1991)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-16  

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