1989 Fiscal Year Annual Research Report

蛋白質機能の分子論的メカニズム

Research Project

Project/Area Number	01101008
Research Institution	Osaka University
Principal Investigator	大沢文夫大阪大学, 名誉教授 (50022497)
Co-Investigator(Kenkyū-buntansha)	京極好正大阪大学, 蛋白質研究所, 教授 (90012632) 朝倉昌名古屋大学, 理学部, 教授 (80022531) 小川英行大阪大学, 理学部, 教授 (70028207) 籏野昌弘東北大学, 非水溶液化学研究所, 教授 (50006293) 香川靖雄自治医科大学, 教授 (30048962)
Keywords	蛋白質分子 / 立体構造形成 / 蛋白質の機能と構造 / 蛋白質分子集合体の機能 / 蛋白質の高次構造解析 / 蛋白質構造と遺伝子操作 / 分子機械 / 分子認識
Research Abstract	特定研究「蛋白質機能の分子論的メカニズム」は昭和61年から平成元年にかけて設定されて、多大の研究成果を納めた。本総合研究(A)は、その研究成果の取纏めを行い、次のような公開シンポジウムの開催と印刷物の出版を行った。 [公開シンポジウムの開催]「蛋白質“すがた、かたち"と“はたらき"」と題する公開シンポジウムを、2月13日と同14日にわたって東京ガ-デンパレスで開催した。この特定研究で活躍した若い研究者を主体にして、これまでの成果とともにこれからの展望を熱心に討論した。 [印刷物の出版]1.特定研究で得られた成果を、“Dynamics(Switching and Movements)of Protein Molecules and Their Assemblies"と題する英文総説集にまとめ、Advances Biophysics Vol.26として学会出版センタ-から出版した。 2.公開シンポジウムの講演とそれに対する討論内容を、和文の単行本、「蛋白質“すがた、かたち"と“はたらき"」にまとめ出版した。来年度も、市販もされる。 3.論文集の発行。本特定研究に参加した研究者全員が、本特定研究の成果を発表した自著論文の中の珠玉の一編を提出して、それを冊子に編集し関係者に配布した。 4.全論文リストの発行。本特定研究の参加者全員が本研究に関連して発表した全論文のリストを冊子にまとめ関係者に配布した。 5.名簿作成。今後の一層の研究交流のため、本特定研究参加者全員の最新の名簿を作り配布した。

Research Products

(99 results)

All Other

All Publications (99 results)

[Publications] Sakamoto,T.,H.Nakamura,H.Uedaira & A.Wada: "High-frequency dielectric relaxation of water bound to hydrophilic silicagels." J.Phys.Chem.93. 357-366 (1989)
[Publications] Wada,A.,S.Kidokoro & S.Endo: "Expanding roles of computers robotics in biological macromolecular research." Ann.Rev.Biophys.Biophys.Chem.18. 1-24 (1989)
[Publications] Shimizu,T.,Sadeque,Abu Jafar MD.,M.Hatano & Fjii: "Binding of axial ligands to cytochrome p-450 mutants;a difference absorption spectral study." Biochim.Biophys.Acta. 995. 116-121 (1989)
[Publications] Ohta,M.,Nozawa,M.Hatano,H.Hayashi,M.Tasumi & K.Shimada: "^<31>P-NMR studies of photophosphorylation in chromatophores from Chromatium vinosum." Biochim.Biophys.Acta. 1011. 33-39 (1989)
[Publications] Furuya,H.,T.Shimizu,M.Hatano & Fjii-Kuriyama,Y.: "Mutations at the distal and proximal sites of cytochrome p-450_d changed regio-specificity of acetanilide hydroxylations." Biocheim.Biophys.Res.Commun.160. 669-676 (1989)
[Publications] Ookubo,S.,T.Nozawa,& M.Hatano: "Solvent effect on MCD of F(111)heme complexes:magnetic circular dichroism spectra of five-coordinated high-spin iron(111)protoporphyrin-lX-di-methylester in the visible region and their environmental effect." J.Inorganic Biochem.35. 305-317 (1989)
[Publications] Furuya,H.,T.Shimizu,K.Hirano,M.Hatano,R.Raag & T.Poulos: "Site-directed mutageneses of rat liver cytochrome p-450:Catalytic activities toward benzphetamine and 7-ethoxycoumarin." Biochemistry. 28. 6848-6857 (1989)
[Publications] Konami,H.,M.Hatano & A.Tajiri: "An analysis of paramagnetic shifts in proton NMR spectra of non-radical lanthanide(111)-phthalocyanine sandwich complexes." Chem.Phys.Lett.160. 163-167 (1989)
[Publications] Sotokawa,H.,T.Shimizu,H.Furuya,M.Hatano,Y.Ohba,M.Iwaizumi & Y.Fjii,Kuriyama: "Electron spin resonance studies of wild-type and mutant cytochromes p-450:effects of mutations at proximal,aromatic and distal sites on values." Biochim.Biophys.Acta. in press. (1989)
[Publications] Konami,H.,M.Hatano,N.,Kobayashi & T.Osa: "Redox potentials of a series of lanthanide-bisphthalocyanine sandwich complexes." Chem.Phys.Lett.in press. (1990)
[Publications] Konami,H.,M.Hatano & A.Tajiri: "Inter-ring overlap integrals in dimer complexes of phthalocyanines and porphyrins." Chem.Phys.Lett.in press. (1990)
[Publications] Noguti,T.,& N.Go: "Structural basis of hierarchical substates of a protein.1" Proteins. 5. 97-103 (1989)
[Publications] Noguti,Y.& N.Go: "Structural basis of hierarchical multiple substates of a protein.II:Monte carlo simulation of native thermal fluctuation and energy minimization." Proteins. 5. 104-112 (1989)
[Publications] Noguti,T.,& N.Go: "Structural basis of hierarchical multiple substates of a protein.III Sidechain and mainchain local conformations." Proteins. 5. 113-124 (1989)
[Publications] Noguti,T.,& N.Go: "Structural basis of hierarchical multiple substates of a protein.IV:Rearrangements in atom packing and local deformations." Proteins. 5. 125-131 (1989)
[Publications] Noguti,T.,& N.Go: "Structural basis of hierarchical multiple substates of a protein.V:Nonlocal deformations." Proteins. 5. 132-138 (1989)
[Publications] Higo,J.,& N.Go: "Algorithm for rapid calculation of excluded volume of large molecules." J.Computational Chem.10. 376-379 (1989)
[Publications] Inagaki,F.,I.Shimada,K.,Kawaguchi,M.,Hirano,I.,Terasawa,T.,Ikura & N.Go: "Structure of melittin bound to perdeuterated dodecylphosphocholine micelles as studied by 2D-NMR and distance geometry calculation." Biochemistry. 25. 5985-5991 (1989)
[Publications] 郷信広: "蛋白質研究のフロンティア." 生物物理のフロンティア. 3. 14 (1989)
[Publications] 木寺詔紀、郷信広: "蛋白質の高次構造予測." 分子生物学の進歩. (1990)
[Publications] 伊倉貞吉、郷信広: "蛋白質の溶液構造-NMRと距離幾何法とによるアプロ-チ" 高分子. (1990)
[Publications] Go,N.,& T.Noguchi: "Structural basis of hierarchical multiple substates of a protein." Chemica Scripta. (1990)
[Publications] Kidera,A.,& N.Go: "Refinement of protein dynamic structure:Normal mode refinement." Proc.Natl.Acad.Sci.USA. (1990)
[Publications] G,N.: "A theotem on amplitudes of thermal stomic fluctuations in large molecules assuming specific conformations calculated by normal mode analysis." Biophysical Chem.(1990)
[Publications] Watanabe,W.,G.Sampei,A.Aiba & K.Mizobuchi: "Identification and sequence analysis of Escherichia coli purE genes encoding 5'-phosphoribosyl-5-amino-4-imidazole carboxylase for de novo purine biosynthesis." J.Bacteriol.171. 198-204 (1989)
[Publications] Sampei,G.,& K.Mizobuchi: "The organization of the purL gene encoding 5'-phosphoribosylformylgly-cinamide amidotransferase of Escherichia coli." J.Biol.Chem.264. 21230-21238 (1989)
[Publications] Aiba,A.,& K.Mizobuchi: "Nucleotide sequence analysis of genes purH and purD involved in the de novo purine nucleotide biosynthesis of Escherichia coli." J.Biol.Chem.264. 21239-21246 (1989)
[Publications] Yonetani,Y.,G.Sampei,K.Shiba & K.Mizobuchi: "PUR box and purR,Regulatory elements involved in the purine and pyrimidine metabolisms." Proc.Natl.Acad.Sci.USA. in press. (1990)
[Publications] Hama,C.,T.Takizawa,H.Moriwaki,Y.Urasaki & K.Mizobuchi: "The organization of replication control region of the ColIb-p9 plasmid." J.Bacteriol.172. (1990)
[Publications] Shiba,K.,& K.Mizobuchi: "Post-transcriptional control of repZ gene expression of the ColIb-p9 plasmid by a small RNA." J.Bacteriol.172. (1990)
[Publications] Honda,H.,& S.Asakura: "Calcium-triggered movement of regulated actin in vitro.A fluorescence microscopy study." J.Mol.Biol.205. 677-683 (1989)
[Publications] Otsuka,M.,S.Ohta,J.Kaneko,K.Nijima,Y.Mizuno,M.Yoshida & Y.Kagawa: "Increased density of mitochondrion isolated from the skeletal muscle of a patient with mitochondrial myopathy." Biomedical Research. 9. 535-540 (1989)
[Publications] Ikura,T.,N.Go,& F.Inagaki: "Refined structure of melittin of melittin bound to perdeuterated dodecylphosph-coline micells as studied by 2D-NMR and distance geometry calculation." Proteins. (1990)
[Publications] Ishihara,A.,N.Fujita,K.Igarashi & R.Ueshima: "Structural and function modulation of Escherichia coli RNA polymerase.In:“Structure and function of nucleic acids proteins"" Raven Press,New York. (1989)
[Publications] Igarashi,K.,N.Fujita & A.Ishihama: "Promoter selectivity of Escherichia coli RNApolymerase:Omega factor is responsible for the ppGpp sensitivity." Nucleic Acids Res.(1989)
[Publications] Ishihama,A.,N.Fujita,K.Igarashi & R.Ueshima,M.Nakayama & Y.Yamazaki: "Molecular mechanisms of transcription regulation:Promoter selectivity of RNA polymerase." FAOB Congress Proceedings.(1989)
[Publications] Nakayama,M.,N.Fujita,T.Ohama,S.Osawa & A.Ishihama: "Micrococcus luteus, a bacterium with a high G+C content,contains Escherichia coli-type promoters." Mol.Gen.Genet.218. 384-389 (1989)
[Publications] Ueshima,R.,N.Fujita & A.Ishihama: "DNA supercoiling and temperrature shift affect the promoter activity of the Escherichia coli rpoH gene encoding the heat-shock sigma subunit of RNA polymerase." Mol.Gen.Genet.215. 185-189 (1989)
[Publications] Higashitani,A.,S.Tabata,T.Ogawa,H.Ogawa M.Shibata & Y.Hotta: "ATP-independent strand transfer protein from murine spermatocytes,and spermatozoa.Exptl." Cell Res.(1990)
[Publications] Ogawa,H.,& T.Ogawa: "Reguation in repressor inactivation by RecA protein." Adv.Biophys.26. (1990)
[Publications] Endo,H.,K,Hasegawa,K.Narisawa,K.Tada,Y.Kagawa & S.Ohta: "Defectiv gene in lactic acidosis:Abnormal phyruvate dehydrogenase E1 alpha snbunit caused by a frame shift." Am.J.Human Genet.44. 358-364 (1989)
[Publications] Muneyuki,E.,Y.Kagawa & H.Hirata: "Steady state kinetics of proton plannar bilayer membranes." J.Biol.Chem.264. 6092-6096 (1989)
[Publications] Kagawa,Y.,S.Ohta,M.Yohda,H.Hamamoto & H.Hirata: "Biotechnological applications of thermophilic ATP synthase:Membrane electronics and genetics." J.Membrane Sci.41. 237-247 (1989)
[Publications] Kagawa,Y.,S.Ohta & Otawara-Hamamoto,Y.: "α3β3 complex of thermophilic ATP synthase:Catalysis without the τ subnit." FEBS Lett.249. 67-69 (1989)
[Publications] Shimoizumi,H.,M.Yoshida-Momoi,S.Ohta,T.Momoi & M.Yanagisawa: "Cytochrome c oxidanse-deficient myogenic cell lines in mitochondtrial myopathy." Ann.Neurol.25. 615-621 (1989)
[Publications] Endo,H.,K.Narisawa,K.Tada,Y.Kagawa & S.Ohta: "Cloning of a defective gene encoding the pyruvate dehydrogenase E1 alpha subunit from a patient with its deficiency." J.Inherit.Metabol.Disease. (1989)
[Publications] Ohta,S.,H.Endo,K.Matsuda & Y.Kagawa: "Gene structure of the promotor region for the human pyruvate dehydrogenase E1 alpha subunit and its gene expression." Ann.N.Y.Acad.Sci.
[Publications] Endo,H.,K.Hasegawa,K.Narisawa,K.Tada,Y.Kagawa & S.Ohta: "Identification of the gene for pyruvate dehydrogenase E1 alpha-subunit with its deficiency." Ann.N.Y.Acad.Sci.
[Publications] Yuzaki,M.,Ohkoshi,I.Kanazawa,Y.Kagawa & S.Ohta: "Multiple deletions in mitochondrial DNA at direct repeats of non-D-loop regions in cases of familial mitochondtrial mypathy." Biochem.Biophys.Res.Commun.
[Publications] Yoshimura,H.,S.Endo,M.Matsumoto,K.Nagayama & Y.Kagawa: "Hexagonal structure of two dimensional crystal of α3β3 complex of thermophilic ATP syntase." J.Biochem.(1989)
[Publications] Kagawa,Y.,M.Oshima & T.Takaoka: "Lipid metabolism and energy transduction of cells cultured in serum free synthetic media." In.Adv.Membrane Fluidity. 8. (1990)
[Publications] Kagawa,Y.,S.Ohta,H.Hirata,T.Hamamoto & I.Karube: "Stable oligomeric proteins for biosensors:Protein Chemistry,gene engineering and electronics.In Bionstrumentation:" Springer-Verlag,Berlin.(1990)
[Publications] Kagawa,Y.& S.Ohta: "Regulation of mitochondrial ATP synthesis in mammalian cells by transcriptional control." Internatl.J.Biochem.
[Publications] Ito,Y.,M.Harada,S.Ohta,Y.Kagawa,O.Aono,J.Schefer & B.P.Schoenborn: "Small-angle neutron scattering studies of the reconstituted TFI of H+-ATPase from thermophilic bacterium PS3 with deuterated subunits." J.Mol.Biol.
[Publications] Arai,T.,& G.Matsumoto: "“Axolinin localization in the nervous tissue of squid revealed by monoclonal antibodies specific for axolinin:Cellular snd subucellular Iocalization of axolinin in the squid neuron"" J.Neurochem.52. 93-100 (1989)
[Publications] Matsumoto,G.,S.Tsukita & T.Arai: "Organization of the axonal cytoskeleton:Differentiation of the microtubule and action filament arrays." Cell Movement. 2. 335-356 (1989)
[Publications] Fujiwara,T.,Y.Kobayashi,Y.Kyogoku,M.Kuwabara,M.Kodama & S.Seki: "Behavior of water molecules associated with phase transitions in the binaly system of dioctadecyldimecylammonium chloride and water studied by proton and deuterium magnetic resonances." J.Colloid.& Interface Sci.127. 26-34 (1989)
[Publications] Hayashi,F.,S.Enco,Y.Arata,A.Shimizu & Y.Kyogoku: "Creation of a nuclear magnetic resonance data repository and literature database." Prot.Seq.Data Analysis. 2. 23-37 (1989)
[Publications] Hayashi,F.,S.Enco,Y.Arata,A.Shimizu & Y.Kyogoku: "Photo-CIDNP studies of bence jones proteins,immunoglobulins and their proteolytic fragments." Biochemistry. 28. 3976-3981 (1989)
[Publications] Koyma,S.,A.Sato,M.Kobayashi,Y.Kobayashi,T.Ohkubo,Y.Kyogoku & N.Go: "Conformational difference betweenh ANP and met(0)12-hANP." Peptides. 424-428 (1989)
[Publications] Koboyashi,Y.,T,Ohkubo,Y.Kyogoku,Y.Nishiuchi,S.Sakakibara,W.Braun & N.Go: "Solution conformation of conotoxin GI determined by ^1H nuclear magnetic resonance spectroscopy and distance geometry calculations." Biochem.28. 4853-4860 (1989)
[Publications] Nakashima,Y.,M.Muto,K.Kawano,Y.Kyogoku & Y.Yoshikawa: "Nitrogen-15 NMR chemical shifts in metal-ammine complexes.11.Cobalt(III)ammine and amine complexes." Bull.Chem.Scc.Jpn.62. 2455-2460 (1989)
[Publications] Katahira,M.,H.Sugeta & Y.Kyogoku: "A new model for DNA bending on the basis of gradual compression of the minor groove from 5'to 3'along the oligo(dA)tract." Nucleic Acids Res.69-70 (1989)
[Publications] Kushiba,T.,J.S.Ahn,K.Hirata & A.Kurita: "Glass transition of deoxymyoglobin probed by optical absorption spectroscopy." Biochem.Biophys.Res.Commun.160. 948-953 (1989)
[Publications] Kurita,A.,Y.Kanematsu & T.Kushida: "Nonphotochemical hole-burning in zn-substituted myoglobin." J.Lumin.
[Publications] Kushida,T.,A.Kurita,Y.Kanematsu & Y.Touyama: "Hole-burning kinetics in biomolecules and polymers." Canadian J.Phys.
[Publications] 磯晃二郎: "パソコンによるタンパク質・核酸の分子グラフィックス.-その後の進歩と一般有機化合物の表示まで-" 生物物理. (1990)
[Publications] 磯晃二郎: "バイオマテリアル開発への電算機の応用.1.2.3" PDB(プロテイン・デ-タ・バンク)(株)アイピ-シ-. (1990)
[Publications] 磯晃二郎: "バイオマテリアル開発への電算機の応用.1.3.1." パソコングラフィックス(株)アイピ-シ-. (1990)
[Publications] Oobatake,M.& T.Ooi: "Characteristic roles of hydrated water for 20 amino acid residues in stabilization of globular proteins." Bull.Inst.Chem.Res.,Kyoto Univ.66. 433-445 (1989)
[Publications] Otaka,E.& T.Ooi: "Examination of proteins sequence homologies:V.New perspectives on evlution between bacterial and chloroplastーtype ferredoxins inferred from sequence evolution evidence." J.Mol.Evol.29. 246-254 (1989)
[Publications] Otaka,E.,T.Ooi & K.Suzuki: "Examination of proteins sequence homologies:VI.te evolution of Escherichia coli L7/L12 equivalent ribosomal(‘A'proteins)and the tertiary sturcture." Protein seq.Data Anal.2. 395-402 (1989)
[Publications] Takahashi,S.,E.Kim,T.Hibino & T.Ooi: "Comparison of α-helix stability in peptides having a negatively or positively charged residue block attached either to the N-or C-terminus of an α-helix:The electrostatic contribution and anisotropic stability of the α-helix." Biopolymers. 28. 995-1009 (1989)
[Publications] Hotani,H.: "Cynamic structures of liposomes visualized by video microscopy." J.Dispers.Sci.Technolo.10. 441-454 (1989)
[Publications] Mandelkow,E.,Mandelkow E-M,Hotani,H.,B.Hess,& S.C.Muller: "Spatial pattern from oscillating microtubules." Science. 246. 1291-1293 (1989)
[Publications] Itoh,T.J,& H.Hotani: "MAPs dependent regulation of the dynamic instability of microtubules in“Dynamics of Microtubules"ed," Hotani,H.,1989 The Taniguchi Foundation. 187-203 (1989)
[Publications] Miyamoto,H.& H.Hotani: "Polymerization of microtubule within liposomes produces morphological change of their shapes." In“Dynamic of Microtubules"ed,H.Hotani The taniguchi Foundation. 220-242 (1989)
[Publications] Hotani,H.& T.Ikeda: "Dynamic features of biomolecular assemblies visualized by dark-field light microscopy." In“X-ray Microscopy in Biology and Medicine"ed.Shinohara,K.,Yada,K.,Kihara,K.,& Saitoh,T.,1990 Japan Sci.Soc.Pub.Inc & Springer-Verlag Inc.269-276 (1990)
[Publications] Sekimura,T.& H.Hotani: "The morphogenesis of liposomes viewed from the aspect of bending energy." J.Theor.Biol.(1990)
[Publications] 室谷紘一: "生体の超分子構造と機能" 高分子. 38. 412-415 (1989)
[Publications] 室谷紘一: "微小管の形成・解体による構造形成制御" 蛋白質核酸酵素. 34. 1455-1461 (1989)
[Publications] 室谷紘一、伊藤知彦: "微小管の動的性質細胞増殖・細胞運動(上代淑人、矢原一郎編)" 分子生物学の進歩. 7. 203-221 (1989)
[Publications] 室谷紘一: "暗視野顕微鏡限界を超える生物顕微鏡(室谷紘一、木下一彦編)" 分光学生測定法シリ-ズ.
[Publications] Yanagida,T.,& Y.Harada: "The elementary process in the actomyosin energy transduction system" Muscle Energetics Alan R.Liss,Inc.27-36 (1989)
[Publications] 江橋節郎: "薬理学研究の回顧と展望" 日薬理誌. 93. 1-5 (1989)
[Publications] Ebashi,S.: "Calcium regulation of smooth muscle contraction,In Muscle Energetics" Alan R.Liss,Inc.New York,. 239-251 (1989)
[Publications] Ebashi,S.: "Development of Ca^<2+> concept in smooth muscle research.In Frontiers in smooth muscle research" Alan R.Liss,Inc.New York,. 159-165 (1990)
[Publications] Satake,K.,M.Yugi,Kamo,H.Kihara & A.Tsugita: "Hemerythrin fro lingula unguis consists of two different subunits, alpha and beta." Protein Sequence and Data Analysis. (1989)
[Publications] Fuseya,M.,K.Ichikawa,T.Yamamura,Y.Tachiiri,K.Satake,Y.Amemiya & H.Kihara: "Dissociation and auto-oxidation of hemerythrin induced by SH-modification." Kinetic Study.J.Biochem.105. 293-298 (1989)
[Publications] Wang,Z.X.,H.Tsuruta,Y.Honda,Y.Tachiiri,Y.Wakabayashi,Y.Amemiya & H.Kihara: "Kinetic study on the dimertetramer interconversion of phosphorylase by stoppedーflow X-ray scattering method." Biophys.Chem.33. 153-160 (1989)
[Publications] Ichimura,K.,T.Yamamura,H.Kihara & K.Satake: "Negative cooperativity of chicken ovotransferrin in AI(III)-binding." J.Biochem.106. 50-54 (1989)
[Publications] Satake,K.F.Tambo,K.Ichimura,M.Fuseya,S.Takahashi,& H.Kihara: "Auto-oxidation of lingulaunguis and siphonosoma cumanese hemerythrins induced by some perturbants." Biochem.International.18. 311-317 (1989)
[Publications] Tsuruta,H.,T.Nagamura,K.Kimura,Y.Igarashi,A.Kajita & H.Kihara: "Stopped-flow apparatus for X-ray scattering at subzero temperature." Rev.Sci.Instr.60. 2356-2358 (1989)
[Publications] Kimura,K.,Y.Igarashi,A.Kajita,Z.-X.Wang,H.Tsuruta,Y.Amemiya,& H.Kihara: "Dissociation of limulus polyphemus(horseshoe crab)hemocyanin monitored by stopped-flow X-ray scattering method." PF Activity Report. 6. 133 (1989)
[Publications] Igarashi,Y.,K.Kimura,A.Kajita,H.Tsuruta,Y.Amemi & H.Kihara: "Dissociation of eisenia foetida(eartworm)Hemoglobin monitored by stopped-flow X-ray scattering combined with time-resolved imaging plate as detector." Pf Activity Report. 6. 134 (1989)
[Publications] Tsuruta,H.,A.Ishihama,N.Fujita,Y.Amemiya & H.Kihara: "Dissociation and assoication kinetics of E.coli ribosome.Stopped-flow X-ray scattering study at subzero temperature." PF Activity Report. 6. 131 (1989)
[Publications] Ikai,A.,H.Arakawa,H.Kyushiki,Y.Tachiiri,H.Kihara,H.Tsuruta & Y.Amemiya: "Structural synamics of alpha-2-macrobloglin and fatty acid synthetase sudied by time-resolved X-ray small angle scattering experiment." PF Activity Report. 6. 132 (1989)
[Publications] Kihara,H.: "Why is X-ray microscopy necessary for biology in“X-ray microscopy in biology and medicine(ed.by K.Shinihara et al.)" Japan Scientific Societies Press,Tokyo. (1989)
[Publications] Kihara,H.,Y.Shimanuki,K.Kawasaki,Y.Watanabe,S.Ogura,H.Tsuruta & Y.Nagai: "VUV microscope with free-standing Zone plates at UVSOR,in“X-ray microscopy in biology and medicine(ed,by K.Shinohara et al.)" Japan Scientific Socienties Press,Tokyo. (1989)

1989 Fiscal Year Annual Research Report

蛋白質機能の分子論的メカニズム

Principal Investigator

大沢 文夫 大阪大学, 名誉教授 (50022497)

Research Products

[Publications] Sakamoto,T.,H.Nakamura,H.Uedaira & A.Wada: "High-frequency dielectric relaxation of water bound to hydrophilic silicagels." J.Phys.Chem.93. 357-366 (1989)

[Publications] Wada,A.,S.Kidokoro & S.Endo: "Expanding roles of computers robotics in biological macromolecular research." Ann.Rev.Biophys.Biophys.Chem.18. 1-24 (1989)

[Publications] Shimizu,T.,Sadeque,Abu Jafar MD.,M.Hatano & Fjii: "Binding of axial ligands to cytochrome p-450 mutants;a difference absorption spectral study." Biochim.Biophys.Acta. 995. 116-121 (1989)

[Publications] Ohta,M.,Nozawa,M.Hatano,H.Hayashi,M.Tasumi & K.Shimada: "^<31>P-NMR studies of photophosphorylation in chromatophores from Chromatium vinosum." Biochim.Biophys.Acta. 1011. 33-39 (1989)

[Publications] Furuya,H.,T.Shimizu,M.Hatano & Fjii-Kuriyama,Y.: "Mutations at the distal and proximal sites of cytochrome p-450_d changed regio-specificity of acetanilide hydroxylations." Biocheim.Biophys.Res.Commun.160. 669-676 (1989)

[Publications] Ookubo,S.,T.Nozawa,& M.Hatano: "Solvent effect on MCD of F(111)heme complexes:magnetic circular dichroism spectra of five-coordinated high-spin iron(111)protoporphyrin-lX-di-methylester in the visible region and their environmental effect." J.Inorganic Biochem.35. 305-317 (1989)

[Publications] Furuya,H.,T.Shimizu,K.Hirano,M.Hatano,R.Raag & T.Poulos: "Site-directed mutageneses of rat liver cytochrome p-450:Catalytic activities toward benzphetamine and 7-ethoxycoumarin." Biochemistry. 28. 6848-6857 (1989)

[Publications] Konami,H.,M.Hatano & A.Tajiri: "An analysis of paramagnetic shifts in proton NMR spectra of non-radical lanthanide(111)-phthalocyanine sandwich complexes." Chem.Phys.Lett.160. 163-167 (1989)

[Publications] Sotokawa,H.,T.Shimizu,H.Furuya,M.Hatano,Y.Ohba,M.Iwaizumi & Y.Fjii,Kuriyama: "Electron spin resonance studies of wild-type and mutant cytochromes p-450:effects of mutations at proximal,aromatic and distal sites on values." Biochim.Biophys.Acta. in press. (1989)

[Publications] Konami,H.,M.Hatano,N.,Kobayashi & T.Osa: "Redox potentials of a series of lanthanide-bisphthalocyanine sandwich complexes." Chem.Phys.Lett.in press. (1990)

[Publications] Konami,H.,M.Hatano & A.Tajiri: "Inter-ring overlap integrals in dimer complexes of phthalocyanines and porphyrins." Chem.Phys.Lett.in press. (1990)

[Publications] Noguti,T.,& N.Go: "Structural basis of hierarchical substates of a protein.1" Proteins. 5. 97-103 (1989)

[Publications] Noguti,Y.& N.Go: "Structural basis of hierarchical multiple substates of a protein.II:Monte carlo simulation of native thermal fluctuation and energy minimization." Proteins. 5. 104-112 (1989)

[Publications] Noguti,T.,& N.Go: "Structural basis of hierarchical multiple substates of a protein.III Sidechain and mainchain local conformations." Proteins. 5. 113-124 (1989)

[Publications] Noguti,T.,& N.Go: "Structural basis of hierarchical multiple substates of a protein.IV:Rearrangements in atom packing and local deformations." Proteins. 5. 125-131 (1989)

[Publications] Noguti,T.,& N.Go: "Structural basis of hierarchical multiple substates of a protein.V:Nonlocal deformations." Proteins. 5. 132-138 (1989)

[Publications] Higo,J.,& N.Go: "Algorithm for rapid calculation of excluded volume of large molecules." J.Computational Chem.10. 376-379 (1989)

[Publications] Inagaki,F.,I.Shimada,K.,Kawaguchi,M.,Hirano,I.,Terasawa,T.,Ikura & N.Go: "Structure of melittin bound to perdeuterated dodecylphosphocholine micelles as studied by 2D-NMR and distance geometry calculation." Biochemistry. 25. 5985-5991 (1989)

[Publications] 郷信広: "蛋白質研究のフロンティア." 生物物理のフロンティア. 3. 14 (1989)

[Publications] 木寺詔紀、郷信広: "蛋白質の高次構造予測." 分子生物学の進歩. (1990)

[Publications] 伊倉貞吉、郷信広: "蛋白質の溶液構造-NMRと距離幾何法とによるアプロ-チ" 高分子. (1990)

[Publications] Go,N.,& T.Noguchi: "Structural basis of hierarchical multiple substates of a protein." Chemica Scripta. (1990)

[Publications] Kidera,A.,& N.Go: "Refinement of protein dynamic structure:Normal mode refinement." Proc.Natl.Acad.Sci.USA. (1990)

[Publications] G,N.: "A theotem on amplitudes of thermal stomic fluctuations in large molecules assuming specific conformations calculated by normal mode analysis." Biophysical Chem.(1990)

[Publications] Watanabe,W.,G.Sampei,A.Aiba & K.Mizobuchi: "Identification and sequence analysis of Escherichia coli purE genes encoding 5'-phosphoribosyl-5-amino-4-imidazole carboxylase for de novo purine biosynthesis." J.Bacteriol.171. 198-204 (1989)

[Publications] Sampei,G.,& K.Mizobuchi: "The organization of the purL gene encoding 5'-phosphoribosylformylgly-cinamide amidotransferase of Escherichia coli." J.Biol.Chem.264. 21230-21238 (1989)

[Publications] Aiba,A.,& K.Mizobuchi: "Nucleotide sequence analysis of genes purH and purD involved in the de novo purine nucleotide biosynthesis of Escherichia coli." J.Biol.Chem.264. 21239-21246 (1989)

[Publications] Yonetani,Y.,G.Sampei,K.Shiba & K.Mizobuchi: "PUR box and purR,Regulatory elements involved in the purine and pyrimidine metabolisms." Proc.Natl.Acad.Sci.USA. in press. (1990)

[Publications] Hama,C.,T.Takizawa,H.Moriwaki,Y.Urasaki & K.Mizobuchi: "The organization of replication control region of the ColIb-p9 plasmid." J.Bacteriol.172. (1990)

[Publications] Shiba,K.,& K.Mizobuchi: "Post-transcriptional control of repZ gene expression of the ColIb-p9 plasmid by a small RNA." J.Bacteriol.172. (1990)

[Publications] Honda,H.,& S.Asakura: "Calcium-triggered movement of regulated actin in vitro.A fluorescence microscopy study." J.Mol.Biol.205. 677-683 (1989)

[Publications] Otsuka,M.,S.Ohta,J.Kaneko,K.Nijima,Y.Mizuno,M.Yoshida & Y.Kagawa: "Increased density of mitochondrion isolated from the skeletal muscle of a patient with mitochondrial myopathy." Biomedical Research. 9. 535-540 (1989)

[Publications] Ikura,T.,N.Go,& F.Inagaki: "Refined structure of melittin of melittin bound to perdeuterated dodecylphosph-coline micells as studied by 2D-NMR and distance geometry calculation." Proteins. (1990)

[Publications] Ishihara,A.,N.Fujita,K.Igarashi & R.Ueshima: "Structural and function modulation of Escherichia coli RNA polymerase.In:“Structure and function of nucleic acids proteins"" Raven Press,New York. (1989)

[Publications] Igarashi,K.,N.Fujita & A.Ishihama: "Promoter selectivity of Escherichia coli RNApolymerase:Omega factor is responsible for the ppGpp sensitivity." Nucleic Acids Res.(1989)

[Publications] Ishihama,A.,N.Fujita,K.Igarashi & R.Ueshima,M.Nakayama & Y.Yamazaki: "Molecular mechanisms of transcription regulation:Promoter selectivity of RNA polymerase." FAOB Congress Proceedings.(1989)

[Publications] Nakayama,M.,N.Fujita,T.Ohama,S.Osawa & A.Ishihama: "Micrococcus luteus, a bacterium with a high G+C content,contains Escherichia coli-type promoters." Mol.Gen.Genet.218. 384-389 (1989)

[Publications] Ueshima,R.,N.Fujita & A.Ishihama: "DNA supercoiling and temperrature shift affect the promoter activity of the Escherichia coli rpoH gene encoding the heat-shock sigma subunit of RNA polymerase." Mol.Gen.Genet.215. 185-189 (1989)

[Publications] Higashitani,A.,S.Tabata,T.Ogawa,H.Ogawa M.Shibata & Y.Hotta: "ATP-independent strand transfer protein from murine spermatocytes,and spermatozoa.Exptl." Cell Res.(1990)

[Publications] Ogawa,H.,& T.Ogawa: "Reguation in repressor inactivation by RecA protein." Adv.Biophys.26. (1990)

[Publications] Endo,H.,K,Hasegawa,K.Narisawa,K.Tada,Y.Kagawa & S.Ohta: "Defectiv gene in lactic acidosis:Abnormal phyruvate dehydrogenase E1 alpha snbunit caused by a frame shift." Am.J.Human Genet.44. 358-364 (1989)

[Publications] Muneyuki,E.,Y.Kagawa & H.Hirata: "Steady state kinetics of proton plannar bilayer membranes." J.Biol.Chem.264. 6092-6096 (1989)

[Publications] Kagawa,Y.,S.Ohta,M.Yohda,H.Hamamoto & H.Hirata: "Biotechnological applications of thermophilic ATP synthase:Membrane electronics and genetics." J.Membrane Sci.41. 237-247 (1989)

[Publications] Kagawa,Y.,S.Ohta & Otawara-Hamamoto,Y.: "α3β3 complex of thermophilic ATP synthase:Catalysis without the τ subnit." FEBS Lett.249. 67-69 (1989)

[Publications] Shimoizumi,H.,M.Yoshida-Momoi,S.Ohta,T.Momoi & M.Yanagisawa: "Cytochrome c oxidanse-deficient myogenic cell lines in mitochondtrial myopathy." Ann.Neurol.25. 615-621 (1989)

[Publications] Endo,H.,K.Narisawa,K.Tada,Y.Kagawa & S.Ohta: "Cloning of a defective gene encoding the pyruvate dehydrogenase E1 alpha subunit from a patient with its deficiency." J.Inherit.Metabol.Disease. (1989)

[Publications] Ohta,S.,H.Endo,K.Matsuda & Y.Kagawa: "Gene structure of the promotor region for the human pyruvate dehydrogenase E1 alpha subunit and its gene expression." Ann.N.Y.Acad.Sci.

[Publications] Endo,H.,K.Hasegawa,K.Narisawa,K.Tada,Y.Kagawa & S.Ohta: "Identification of the gene for pyruvate dehydrogenase E1 alpha-subunit with its deficiency." Ann.N.Y.Acad.Sci.

[Publications] Yuzaki,M.,Ohkoshi,I.Kanazawa,Y.Kagawa & S.Ohta: "Multiple deletions in mitochondrial DNA at direct repeats of non-D-loop regions in cases of familial mitochondtrial mypathy." Biochem.Biophys.Res.Commun.

[Publications] Yoshimura,H.,S.Endo,M.Matsumoto,K.Nagayama & Y.Kagawa: "Hexagonal structure of two dimensional crystal of α3β3 complex of thermophilic ATP syntase." J.Biochem.(1989)

[Publications] Kagawa,Y.,M.Oshima & T.Takaoka: "Lipid metabolism and energy transduction of cells cultured in serum free synthetic media." In.Adv.Membrane Fluidity. 8. (1990)

[Publications] Kagawa,Y.,S.Ohta,H.Hirata,T.Hamamoto & I.Karube: "Stable oligomeric proteins for biosensors:Protein Chemistry,gene engineering and electronics.In Bionstrumentation:" Springer-Verlag,Berlin.(1990)

[Publications] Kagawa,Y.& S.Ohta: "Regulation of mitochondrial ATP synthesis in mammalian cells by transcriptional control." Internatl.J.Biochem.

[Publications] Ito,Y.,M.Harada,S.Ohta,Y.Kagawa,O.Aono,J.Schefer & B.P.Schoenborn: "Small-angle neutron scattering studies of the reconstituted TFI of H+-ATPase from thermophilic bacterium PS3 with deuterated subunits." J.Mol.Biol.

[Publications] Arai,T.,& G.Matsumoto: "“Axolinin localization in the nervous tissue of squid revealed by monoclonal antibodies specific for axolinin:Cellular snd subucellular Iocalization of axolinin in the squid neuron"" J.Neurochem.52. 93-100 (1989)

[Publications] Matsumoto,G.,S.Tsukita & T.Arai: "Organization of the axonal cytoskeleton:Differentiation of the microtubule and action filament arrays." Cell Movement. 2. 335-356 (1989)

[Publications] Hayashi,F.,S.Enco,Y.Arata,A.Shimizu & Y.Kyogoku: "Creation of a nuclear magnetic resonance data repository and literature database." Prot.Seq.Data Analysis. 2. 23-37 (1989)

[Publications] Hayashi,F.,S.Enco,Y.Arata,A.Shimizu & Y.Kyogoku: "Photo-CIDNP studies of bence jones proteins,immunoglobulins and their proteolytic fragments." Biochemistry. 28. 3976-3981 (1989)

[Publications] Koyma,S.,A.Sato,M.Kobayashi,Y.Kobayashi,T.Ohkubo,Y.Kyogoku & N.Go: "Conformational difference betweenh ANP and met(0)12-hANP." Peptides. 424-428 (1989)

[Publications] Koboyashi,Y.,T,Ohkubo,Y.Kyogoku,Y.Nishiuchi,S.Sakakibara,W.Braun & N.Go: "Solution conformation of conotoxin GI determined by ^1H nuclear magnetic resonance spectroscopy and distance geometry calculations." Biochem.28. 4853-4860 (1989)

[Publications] Nakashima,Y.,M.Muto,K.Kawano,Y.Kyogoku & Y.Yoshikawa: "Nitrogen-15 NMR chemical shifts in metal-ammine complexes.11.Cobalt(III)ammine and amine complexes." Bull.Chem.Scc.Jpn.62. 2455-2460 (1989)

[Publications] Katahira,M.,H.Sugeta & Y.Kyogoku: "A new model for DNA bending on the basis of gradual compression of the minor groove from 5'to 3'along the oligo(dA)tract." Nucleic Acids Res.69-70 (1989)

[Publications] Kushiba,T.,J.S.Ahn,K.Hirata & A.Kurita: "Glass transition of deoxymyoglobin probed by optical absorption spectroscopy." Biochem.Biophys.Res.Commun.160. 948-953 (1989)

[Publications] Kurita,A.,Y.Kanematsu & T.Kushida: "Nonphotochemical hole-burning in zn-substituted myoglobin." J.Lumin.

[Publications] Kushida,T.,A.Kurita,Y.Kanematsu & Y.Touyama: "Hole-burning kinetics in biomolecules and polymers." Canadian J.Phys.

[Publications] 磯晃二郎: "パソコンによるタンパク質・核酸の分子グラフィックス.-その後の進歩と一般有機化合物の表示まで-" 生物物理. (1990)

[Publications] 磯晃二郎: "バイオマテリアル開発への電算機の応用.1.2.3" PDB(プロテイン・デ-タ・バンク)(株)アイピ-シ-. (1990)

[Publications] 磯晃二郎: "バイオマテリアル開発への電算機の応用.1.3.1." パソコングラフィックス(株)アイピ-シ-. (1990)

[Publications] Oobatake,M.& T.Ooi: "Characteristic roles of hydrated water for 20 amino acid residues in stabilization of globular proteins." Bull.Inst.Chem.Res.,Kyoto Univ.66. 433-445 (1989)

[Publications] Otaka,E.& T.Ooi: "Examination of proteins sequence homologies:V.New perspectives on evlution between bacterial and chloroplastーtype ferredoxins inferred from sequence evolution evidence." J.Mol.Evol.29. 246-254 (1989)

[Publications] Otaka,E.,T.Ooi & K.Suzuki: "Examination of proteins sequence homologies:VI.te evolution of Escherichia coli L7/L12 equivalent ribosomal(‘A'proteins)and the tertiary sturcture." Protein seq.Data Anal.2. 395-402 (1989)

[Publications] Hotani,H.: "Cynamic structures of liposomes visualized by video microscopy." J.Dispers.Sci.Technolo.10. 441-454 (1989)

[Publications] Mandelkow,E.,Mandelkow E-M,Hotani,H.,B.Hess,& S.C.Muller: "Spatial pattern from oscillating microtubules." Science. 246. 1291-1293 (1989)

[Publications] Itoh,T.J,& H.Hotani: "MAPs dependent regulation of the dynamic instability of microtubules in“Dynamics of Microtubules"ed," Hotani,H.,1989 The Taniguchi Foundation. 187-203 (1989)

[Publications] Miyamoto,H.& H.Hotani: "Polymerization of microtubule within liposomes produces morphological change of their shapes." In“Dynamic of Microtubules"ed,H.Hotani The taniguchi Foundation. 220-242 (1989)

大沢文夫大阪大学, 名誉教授 (50022497)

[Publications] 室谷紘一、伊藤知彦: "微小管の動的性質細胞増殖・細胞運動(上代淑人、矢原一郎編)" 分子生物学の進歩. 7. 203-221 (1989)

[Publications] 室谷紘一: "暗視野顕微鏡限界を超える生物顕微鏡(室谷紘一、木下一彦編)" 分光学生測定法シリ-ズ.