成長ホルモンの分子進化と魚類の分類

1990 Fiscal Year Annual Research Report

• Project/Area Number: 01430021
• Research Institution: Kitastao University
• Principal Investigator: 川内 浩司 北里大学, 水産学部, 教授 (70050523)
• Co-Investigator(Kenkyū-buntansha): RAND Weaver 北里大学, 水産学部, 助手 (20221004)
• Keywords: 成長ホルモン / プロラクチン / ソマトラクチン / 分子系統樹 / 分子進化 / 一次構造 / ハイギヨ / クロコダイル

Research Abstract

本研究は、魚類および水産四足動物の成長ホルモン(GH)およびプロラクチン(PRL)を単離構造決定し、アミノ酸配列に基づいて分子系統樹を作成し、動物、特に魚類の類縁関係を定量化することを目的とする。本年度は、爬虫類:クロコダイル,両生類:ウシガエル,肺魚類:ハイギヨ,全骨類:アミア,真骨魚類:ハゼのGH、哺乳類:クジラ,全骨類:アミア,真骨魚類:ナマズ,ヒラメ、ハゼのPRLを単離し、カエル,クロコダイルのGH,クジラ、ナマズ,ヒラメのPRLの全一次構造を決定した。昨年度、大西洋タラの中葉から発見したGH/PRL分子族の新しいホルモン,ソマトラクチン(SL)をヒラメ、サケ科魚類、ナマズ、カツオの脳下垂体から単離した。ヒラメとシロザケのSLの全一次構造を決定し、糖鎮結合部位とSーS結合位置を同定した。一方,肉鰭類:シ-ラカンス、軟骨魚類:ギンザメ、円口類:ヤツメウナギのGH/PRL/SLについて、哺乳類および魚類のホルモンの抗血清を用いて、イムノブロッティング法で検索した。しかし、免疫反応は極めて微弱かあるいは、試料量が少ないため、精製は極めて困難である。以上の通り、本年度は、爬虫類および両生類の各1種のGH,哺乳類1種,真骨魚類2種のPRL、および真骨魚類2種のSLの全一次構造を決定した。本研究によって、円口類を除く主要な脊椎動物のGHの一次構造が明らかになった。一次構造の比較によって作成したGHの分子系統樹は形態分類の結果とよく符合する。したがって,動物間の類縁関係はアミノ酸配列の変異率〓〓て数値化することができた。また、GH/PRL/SLは、共通の祖先遺伝子がほぼ同時期に重復して、分枝したと推定された。

Research Products

• [Publications] Ono,M.,Takayama,Y.,RandーWeaver,M.,Sakata,S.,Yasunaga,T.,Noso,T.,Kawauchi,H.: "cDNA Cloning of Somatolactin,a New Pituitary Protein Related to Growth Hormone and Prolactin." Proc.Natl.Acad.Sci.USA.87. 4330-4334 (1990)
• [Publications] Rydevik,M.,Borg,B.,Haux,C.,Kawauchi,H.,Bjornsson,B.Th.: "Plasma growth hormone levels increase during seawater exposure of sexually matured Atlantic salmon parrs (<Salmo>___ー <salar>___ー L.)." Gen.Comp.Endocrinol.80. 9-15 (1990)
• [Publications] Yasuda,A.,Kawauchi,H.Papkoff,H.: "The Complete Amino Acid Sequence of Prolactin from the Sea Turtle (Chelonia mydas)." Gen.Comp.Endocrinol.80. 363-371 (1990)
• [Publications] Takahashi,A.,Yasuda,A.,Kawauchi,H.,Hirano,T.: "Development and validation of a competitive enzyme immunoassay for chum salmon prolactin:A comparison to radioimmunoassay." Gen.Comp.Endocrinol.80. 372-380 (1990)
• [Publications] RandーWeaver,M.,Baker,B.I.,Kawauchi,H.: "Cellular Localization of Somatolactin in the Pars Intermedia of Some Teleost Fishes." Cell Tissue Res.
• [Publications] RandーWeaver,M.,Noso,T.,Kawauchi,H.: "The Complete Amino Acid Sequences of Two Variants of Growth Hormone from Atlantic cod (Gadus morhua)." Gen.Comp.Endocrinol.81. 39-50 (1991)
• [Publications] Kobayashi,T.,Yasuda,A.,Yamaguchi,A.,Kawauchi,H.,Kikuyama,S.: "The Complete Amino Acid Sequence of Growth Hormone of the Bullfrog (Rana catesbeiana)." Biochim.Biophys.Acta.
• [Publications] Sakata,S.,Noso,T.,Moriyama,S.,Hirano,T.,Kawauchi,H.: "Flounder Growth Hormone:Isolation,Characterization and Effects on Growth of Juvenile Rainbow Trout." Gen.Comp.Endocrinol.
• [Publications] RandーWeaver,M.,Kawauchi,H.: "Somatolactin:A New Pituitary Homrone Related to Growth Homrone and Prolactin." Kyoto Prolactin Conference Vol.5.
• [Publications] RandーWeaver,M.,Kawauchi,H.: "A Rapid Procedure for the Isolation of Growth Hormone and Prolactin." Analytical Biochemistry.
• [Publications] Campbell,R.M.,Kawauchi,H.,Lewis,U.J.,Papkoff,H.,Scanes,C.G.: "Comparison of lipolytic and antilipolytic activities of lower vertebrate growth hormone on chicken adipose tissue in vitro." Proc.Soc.Exp.Biol.Med.
• [Publications] MacLatchy,D.L.,Kawauchi,H.,Eales,J.G.: "Stimulation of hepatic tyroxine 5'ーdeiodinase activity in rainbow trout by salmon growth hormone." Gen.Comp.Endocrinol.
• [Publications] RandーWeaver,M.,Noso,T.,Muramoto,K.,Kawauchi,H.: "Isolation and Characterization of Somatolactin,a New Protein Related to Growth Hormone and Prolactin from Atlantic Cod (Gadusmorhua) pituitary glands." Biochemistry. 30. 1509-1515 (1991)
• [Publications] Yasuda,A.,Yamaguchi,K.,Kobayashi,T.,Yamamoto,K.,Kikuyama,S.,Kawauchi,H.: "The Complete Amino Acid Sequence of Prolactin from the Bullfrog,Rana catesbeiana." Gen.Comp.Endocrinol.
• [Publications] Suzuki,R.,Yasuda,A.,Kondo,J.,Kawauchi,H.,Hirano,T.: "Isolation and Characterization of Japanese Eel Prolactin." Gen.Comp.Endocrinol.
• [Publications] Takayama,Y.,Ono,M.,RandーWeaver,M.,Kawauchi,H.: "Greater Conservation of Somatolactin,a Presumed Pituitary Hormone of the Growth Hormone/Prolactin Family,than Growth Hormone in Teleost Fish." Gen.Comp.Endocrinol.
• [Publications] Takayama,Y.,RandーWeaver,M.,Kawauchi,H.,Ono,M.: "Gene Structure of Chum Salmon Somatolactin,a Presumed Pituitary Hormone of the Growth Hormone/Prolactin Family." Mol.Endocrinol.
• [Publications] Yasuda,A.,Yamaguchi,K.,Noso,T.,Papkoff,H.,Polenov,A.L.,Nicoll,C.S.,Kawauchi,H.: "The Complete Amino Acid Sequence of Growth Hormone from Sturgeon (Acipencer guldenstadti)." Biochim.Biophys.Acta.
• [Publications] Takahashi,A.,Ogasawara,T.,Kawauchi,H.,Hirano,T.: "Effects of Changes in plasma glucose levels on plasma growth hormone in the immature rainbow trout." Nippon Suisan Gakkaishi.57. 231-235 (1991)
• [Publications] Takahashi,A.,Kawazoe,I.,Kawauchi,H.: "A competitive enzyme immunoassay for chum salmon growth hormone." Nippon Suisan Gakkaishi. 57. 267-272 (1991)
• [Publications] Moriyama,S.,Kawauchi,H.: "Growth Stimulation of Juvenile salmonids by immersion in recombinant salmon growth hormone solution." Nippon Suisan Gakkaishi.56. 31-34 (1990)
• [Publications] Moriyama,S.,Takahashi,A.,Hirano,T.,Kawauchi,H.: "Salmon growth hormone is transported into the circulation of rainbow trout,Salmo gairdneri,after intestinal administration." J.Physiol.Biochem.160. 251-257 (1990)
• [Publications] RandーWeaver,M.,Kawauchi,H.,Ono,M.: "Vertebrate Endocrinology;Fundamental and Biological Imprication Vol.5." P.K.T.Pang,M.P.Schreibman eds.,
• [Publications] Kawauchi,H.: "International Symposium on Biologically Active Proteins and Peptides" S.H.Chiou,K.T.Wang,& S.H.Wu eds.National Taiwan University, 246 (1990)
• [Publications] Kawauchi,H.,Yasuda,A.,RandーWeaver: "Proceedings In Comparative Endocrinology" A.Epple,C.G.Scanes,&M.H.Stetson eds.WileyーLiss,Inc., 752 (1990)
• [Publications] a.Moriyama,S.,Hirano,T.,& Kawauchi,H b.RandーWeaver,M.,Noso,T.,Kawauchi,H: "Current Topics in Marine Biotechnology" S.Miyachi,I.Karube,& Y.Ishida eds.The Japanese Soc.for Marine Biotechnology, 437 (1990)