1990 Fiscal Year Final Research Report Summary
Studies on Iuduction of Autologous MLR in the Autoimmune Diseases and its Pathological Roles.
| Project/Area Number |
01480186
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Immunology
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| Research Institution | Tohoku University |
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Principal Investigator |
KUMAGAI Katsuo Tohoku Univ. Sch. of Dentistry, Professor, 歯学部, 教授 (00005018)
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| Co-Investigator(Kenkyū-buntansha) |
HANAUMI Kiyoshi Tohoku Univ. Sch. of Dentistry, Assistant Prof., 歯学部, 助手 (50005063)
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| Project Period (FY) |
1989 – 1990
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| Keywords | Autologous MLR / DR-antigen / helper T cells / gammadeltaT cells / lupus mouse / autoimmune disease |
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| Research Abstract |
On the specific nature and its biological significance of autologous mixed lymphocyte reaction (MLR), the following results are obtained. (1) Treatment with neuraminidase of the mixtures of stimulator cells and responder lymphocytes results in a marked enhancement of DNA replication in the autologous MLR. (2) Using this method, we are able to demonstrate that the autologous MLR is a helper T (TH) activation induced by DR antigens on accessory (AC) cells, of which the DNA replication is IL-2-independent, but dependent upon the multiple cytokines, IL-3, IL-5, IL-6 and IL-7. (3) The autologous MLR is also found to induce the generation of activated CD4^+ helper T cells, and also CD4^-CD8^- double negative (DN) T cells with a phenotype of alphabetaT cell receptor (alphabetaTcR)^+ or gammadeltaTcR^+. (4) Based on the findings of the specific nature of autologous MLR, the lymphoproliferation. Induced in the lymphoid tissues of MRL/lpr mouse is analyzed. As a result, it is found that abnormal proliferation of CD3^+CD4^-CD8^-B220^+ alphabetaTcR^+T cells in the lymphoid tissues may be a phenomenon in which precursors for this population can be proliferated in response to autologous accessory cells, mediated through certain antigens on the surface.
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[Publications] Abo, T., Sugawara, S., Seki, S, Fujii, M., Rikiishi, H., and Kumagai, K.: "Induction of human TCRgammadelta^+ and TCRgammadelta^-CD2^+CD3^- double negative lymphocytes by bacterial stimulation." Int. Immunol.2. 775-785 (1990)
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「研究成果報告書概要(欧文)」より
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[Publications] Seki, S., Abo, T., Masuda, T., Ohteki, T., Kanno, A., Takeda, K., Rikiishi, H., Nagura, H., and Kumagai, K.: "Identification of activated T cell receptor gammadelta lymphocytes in the liver of tumor-bearing hosts." J. Clin. Invest.86. 409-415 (1990)
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「研究成果報告書概要(欧文)」より
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[Publications] Kawamura, M., Satoh, T., Fujii, N., Abo, T., Rikiishi, H., and Kumagai, K.: "Further characterization of the autologous mixed lymphocyte reaction (AMLR) : induction of double negative gammadelta T lymphocytes." Cell. Immunol.133. (1991)
Description
「研究成果報告書概要(欧文)」より
