1991 Fiscal Year Final Research Report Summary
Analysis of wound healing mechanism at cellular and molecular levels
| Project/Area Number |
01480316
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General surgery
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| Research Institution | Keio university |
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Principal Investigator |
FUJINO Toyomi Keio University School of Medicine, Dept. Plastic Surgery, Professor, 医学部, 教授 (20051279)
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| Co-Investigator(Kenkyū-buntansha) |
MINABE Toshiharu Keio University School of Medicine, Dept. Plastic Surgery, Instructor, 医学部, 助手 (50200077)
KOBAYASHI Masahiro Keio University School of Medicine, Dept. Plastic Surgery, Instructor, 医学部, 助手 (30195812)
SEGAWA Kaoru Keio University School of Medicine, Dept. Microbiology, Assistant Professor, 医学部, 講師 (30114523)
TAKANO Toshiya Keio University School of Medicine, Dept. Microbiology, Professor, 医学部, 教授 (60051364)
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| Project Period (FY) |
1989 – 1991
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| Keywords | Wound healing / Keloid / Collagen synthesis / TGF-beta / Collagenase gene / Immortalization / Collagen mRNA / Regulation of cell proliferation |
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| Research Abstract |
Procollagen ccl, mRNA was specifically expressed at higher levels in the experimental wound tissue on rat dorsal skin five to seven days after the injury. TGF-beta induced the expression of procollagen alphal MRNA at similar levels in the fibroblasts cultured from normal human skin and keloidal tissues. The incorporation of ^<14>C-leucine was three to five more in human fresh keloidal slices than those of normal dermis, suggesting that the hyperproduction of collagen was regulated at a translational level in keloid. The serum of patients with systemic severe keloid strongly stimulated the growth of cultured skin fibroblasts compared with normal human and fetal calf sera. Both higher concentration of growth-stimulating factors and activation of collagen production at a translational level seemed to be involved in the pathogenesis of keloid. In the study on the immortalization of SV40-transformed human diploid fibroblasts as an in vitro modpl of the abnormal proliferation in keloidal skin fibroblasts, certain mutational events responsible for immortalization occurred in crisis and the expression of collagenase gene was specifically shut off after immortalization. The synthesis and degradation of collagen seemed to be intrinsically related with the growth regulation in fibroblasts. In experimental skin pedicle flaps, we detected the prominent dilatation of small anastomotic arteries which seemed to play major roles in supplying blood cells releasing wound-healing factors rather than angiogenesis.
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Research Products
(10 results)
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[Publications] Fujino, T., Kaneko, T., Nakajima, H., Kurihara, T., Kobayashi, M. and Chiyokura, H.: "Progresses in Simulation Surgery (in Japanese)" Abst. 23rd Meet. Japan. Med. Assoc.III. 202-203 (1991)
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「研究成果報告書概要(欧文)」より
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[Publications] Umezawa, A., Tachibana, K., Harigaya, K. . Kusakari, S., Kato, S., Watanabe, Y. and Takano, T.: "Colony-Stimulating Factor I Expression is Down-Regulated During the Adipocyte Differentiation of H-1/A Marrow Stromal Cells and Induced by Cachectin/Tumor Necrosis Factor." Mol. Cell. Biol.11. 920-927 (1991)
Description
「研究成果報告書概要(欧文)」より
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