1991 Fiscal Year Annual Research Report

遺伝子発現の抑制的調節機構:サイレンサ-結合因子とその遺伝子

Research Project

Project/Area Number	01480531
Research Institution	Aichi Cancer Center Research Institute
Principal Investigator	松影昭夫愛知県がんセンター研究所, 生物学部, 部長 (90019571)
Co-Investigator(Kenkyū-buntansha)	山口政光愛知県がんセンター研究所, 生物学部, 主任研究員 (00182460)
Keywords	ショウジョウバエ / DNAポリメラ-ゼα / PCNA / 遺伝子発現 / DRE / DREF / ホメオドメインタンパク質
Research Abstract	今年度は、ショウジョウバエの複製因子であるDNAポリメラ-ゼ(以下polα)とPCNA(増殖細胞各抗原:polδ促進因子)の遺伝子の発現抑制機構の研究に重要な進展があった。 1)両遺伝子は共通の8塩基対TATCGATAの、促進配列(我々はDRE:DNA replicationーrelated elmentと命名した)によって支配されていることを証明したる。さらにこの配列に結合する分子量約85000のタンパク質因子DREFを発見し、その精製法を確立した。現在DREF遺伝子の単離を試みている。 2)昨年、両遺伝子の発現がホメオドメインタンパク質の一つ(zerknullt)によって抑制されることを報告した。この抑制と上記のDRE/DREFとの関係をしらべた結果、zerknulltはDREFの活性低下をもたらすことを証明した。この機構は細胞分化と共役した増殖停止にとって重要であると予想される。zerknulltがDREFを抑制する機構は不明であるが、DREFの遺伝子クロ-ニングおよびそれに対する抗体の作成が重要な手がかりを与えてくれるであろう。

Research Products

(12 results)

All Other

All Publications (12 results)

[Publications] 山口政光: "Distribution of PCNA in Drosophila embryo during nuclear division cycles" J.Cell Science. 100. 729-733 (1991)
[Publications] 山口政光: "Repression of Drosophila proliferating cell nuclear antigen gene promoter by zerknullt protein" Mol.Cell.Biol.11. 4909-4917 (1991)
[Publications] 廣瀬富美子: "Structure and expression during development of Drosophila melanoーgaster gene for DNA polymerase α" Nucleic Acids Res.19. 4991-4998 (1991)
[Publications] 山口政光: "Molecular cloning and structural analysis of mouse gene and pseudogenes for proliferating cell nuclear antigen" Nucleic Acids Res.19. 2403-2410 (1991)
[Publications] 伊達孝保: "Aspartic acid residues at positions 190 and 192 of rat DNA polymerase β are involved in primer binding" Biochemistry. 30. 5286-5292 (1991)
[Publications] 徳井俊也: "Inactivation of DNA polymerase β by in vitro phosphorylation with protein kinase C" J.Biol.Chem.226. 10820-10824 (1991)
[Publications] 山口政光: "Organization of mouse DNA polymerase β gene silencer elements and identification of the silencer binding factor(s)" Jpn.J.Cancer Res.82. 72-81 (1991)
[Publications] Hisanori Suzuki: "Induction of DNA polymerase β during proliferation of mitogenstimulated human lymphocytes" Biochem.Biophys.Res.Commun.181. 623-628 (1991)
[Publications] 山口政光: "Distribution of PCNA in Drosophila embryo during nuclear division cycles" J.Cell Sci.100. 729-733 (1991)
[Publications] 山口政光: "Distribution of DNA polymerase α during nuclear division cycles in Drosophila melanogaster embryo" Cell Struct.Funct.
[Publications] 廣瀬富美子: "Novel 8ーbp sequence(DRE)and specific binding factor(DREF)involved in the expression of Drosophila genes for DNA polymerase α and proliferating cell nuclear antigen" J.Biol.Chem.
[Publications] 山口政光: "Activation of the mouse proliferating cell nuclear antigen gene promoter by adenovirus type 12 E1A proteins" Japn.J.Cancer Res.

1991 Fiscal Year Annual Research Report

遺伝子発現の抑制的調節機構:サイレンサ-結合因子とその遺伝子

Principal Investigator

松影 昭夫 愛知県がんセンター研究所, 生物学部, 部長 (90019571)

Research Products

[Publications] 山口 政光: "Distribution of PCNA in Drosophila embryo during nuclear division cycles" J.Cell Science. 100. 729-733 (1991)

[Publications] 山口 政光: "Repression of Drosophila proliferating cell nuclear antigen gene promoter by zerknullt protein" Mol.Cell.Biol.11. 4909-4917 (1991)

[Publications] 廣瀬 富美子: "Structure and expression during development of Drosophila melanoーgaster gene for DNA polymerase α" Nucleic Acids Res.19. 4991-4998 (1991)

[Publications] 山口 政光: "Molecular cloning and structural analysis of mouse gene and pseudogenes for proliferating cell nuclear antigen" Nucleic Acids Res.19. 2403-2410 (1991)

[Publications] 伊達 孝保: "Aspartic acid residues at positions 190 and 192 of rat DNA polymerase β are involved in primer binding" Biochemistry. 30. 5286-5292 (1991)

[Publications] 徳井 俊也: "Inactivation of DNA polymerase β by in vitro phosphorylation with protein kinase C" J.Biol.Chem.226. 10820-10824 (1991)

[Publications] 山口 政光: "Organization of mouse DNA polymerase β gene silencer elements and identification of the silencer binding factor(s)" Jpn.J.Cancer Res.82. 72-81 (1991)

[Publications] Hisanori Suzuki: "Induction of DNA polymerase β during proliferation of mitogenstimulated human lymphocytes" Biochem.Biophys.Res.Commun.181. 623-628 (1991)

[Publications] 山口 政光: "Distribution of PCNA in Drosophila embryo during nuclear division cycles" J.Cell Sci.100. 729-733 (1991)

[Publications] 山口 政光: "Distribution of DNA polymerase α during nuclear division cycles in Drosophila melanogaster embryo" Cell Struct.Funct.

[Publications] 廣瀬 富美子: "Novel 8ーbp sequence(DRE)and specific binding factor(DREF)involved in the expression of Drosophila genes for DNA polymerase α and proliferating cell nuclear antigen" J.Biol.Chem.

[Publications] 山口 政光: "Activation of the mouse proliferating cell nuclear antigen gene promoter by adenovirus type 12 E1A proteins" Japn.J.Cancer Res.

松影昭夫愛知県がんセンター研究所, 生物学部, 部長 (90019571)

[Publications] 山口政光: "Distribution of PCNA in Drosophila embryo during nuclear division cycles" J.Cell Science. 100. 729-733 (1991)

[Publications] 山口政光: "Repression of Drosophila proliferating cell nuclear antigen gene promoter by zerknullt protein" Mol.Cell.Biol.11. 4909-4917 (1991)

[Publications] 廣瀬富美子: "Structure and expression during development of Drosophila melanoーgaster gene for DNA polymerase α" Nucleic Acids Res.19. 4991-4998 (1991)

[Publications] 山口政光: "Molecular cloning and structural analysis of mouse gene and pseudogenes for proliferating cell nuclear antigen" Nucleic Acids Res.19. 2403-2410 (1991)

[Publications] 伊達孝保: "Aspartic acid residues at positions 190 and 192 of rat DNA polymerase β are involved in primer binding" Biochemistry. 30. 5286-5292 (1991)

[Publications] 徳井俊也: "Inactivation of DNA polymerase β by in vitro phosphorylation with protein kinase C" J.Biol.Chem.226. 10820-10824 (1991)

[Publications] 山口政光: "Organization of mouse DNA polymerase β gene silencer elements and identification of the silencer binding factor(s)" Jpn.J.Cancer Res.82. 72-81 (1991)

[Publications] 山口政光: "Distribution of PCNA in Drosophila embryo during nuclear division cycles" J.Cell Sci.100. 729-733 (1991)

[Publications] 山口政光: "Distribution of DNA polymerase α during nuclear division cycles in Drosophila melanogaster embryo" Cell Struct.Funct.

[Publications] 廣瀬富美子: "Novel 8ーbp sequence(DRE)and specific binding factor(DREF)involved in the expression of Drosophila genes for DNA polymerase α and proliferating cell nuclear antigen" J.Biol.Chem.

[Publications] 山口政光: "Activation of the mouse proliferating cell nuclear antigen gene promoter by adenovirus type 12 E1A proteins" Japn.J.Cancer Res.