1992 Fiscal Year Final Research Report Summary
Transcription Signals and High-ordered Regulatory Mechanisms for Plant Gene Expression
| Project/Area Number |
02304006
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| Research Category |
Grant-in-Aid for Co-operative Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
植物生理学
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
IWABUCHI Masaki Kyoto Univ. Botany Professor, 理学部, 教授 (30000839)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAZAKI Kenichi Nagoya Univ. Agricul. Lecture, 農学部, 助手 (40182480)
UCHIMIYA Hirobumi Tokyo Univ. Applied Microbio. Professor, 応用微生物研究所, 教授 (50142229)
MACHIDA Yasunori Nagoya Univ. Botany Professor, 理学部, 教授 (80175596)
NAKAMURA Kenzo Nagoya Univ. Agri. Professor, 農学部, 教授 (80164292)
MINAMIKAWA Takao Tokyo Metro. Univ. Botany Professor, 理学部, 教授 (30087001)
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| Project Period (FY) |
1990 – 1992
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| Keywords | Plant genes / Transcription Regulation / Cis-elements / DNA-binding proteins / Cell function / Highly ordered regulation mechanism / Promoter structure / Gene expression specificity |
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| Research Abstract |
The aim of the present cooperation research (A) was to investigate the regulatory mechanisms of plant gene expression though the interaction between cis-acting DNA sequences and trans-control factors. Each partaker used his own experimental system and has identified gene-specific regulatory DNA sequences and the sequence-specific DNA binding proteins.
The cis-elements defining the S phase-specific expression of wheat H3 gene were indentified to be the hexameric and octameric motifs in the proximal promoter region and the hexamer-specific DNA-binding protein, HBP-1a(17), was shown to be a bZIP-type protein by sequence analysis of the cDNA clone. The major cis-regions of a concanavalin A gene of Canavalina gladiata was shown to be located downstream of -745 and some DNA-binding proteins that can interact with the regions were also identified. The DNA sequences conferring the induction of high-level of expression of a sweet potato alpha-amylase and sporamin genes was identified. The DNA-binding factor SP8BF was demonstratedto bind to a CGTCA motif in the 5'-upstream region of the alpha-amylase gene. One of the auxin-induced genes, par gene, was isolated and the positive or negative cis-regions were found. DNA-binding proteins that bind to the cis-regions of rolC gene in Ri plasmid were identified.
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