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1991 Fiscal Year Final Research Report Summary

Systematic Approach for the Development of Chemically Reliable Method to Evaluate Lipid Peroxidation in Foods and Biological System.

Research Project

Project/Area Number 02453148
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 家政学
Research InstitutionHyogo University of Teacher Education

Principal Investigator

KOJO Shosuke  Hyogo University of Teacher Education, Department of Life and Health Sciences, Associate Professor, 学校教育学部, 助教授 (10108988)

Co-Investigator(Kenkyū-buntansha) KISHIDA Etsu  Hyogo University of Teacher Education. Department of Life and Health Sciences, A, 学校教育学部, 助手 (70214773)
Project Period (FY) 1990 – 1991
KeywordsLipid peroxidation / Malondialdehyde / TBA-reactive substances / Ascorbic acid (Vitamin C) / Tocopherol (Vitamin E) / Chemiluminescence / Glutathione / Carbon tetrachloride
Research Abstract

(1) We established a specific and highly sensitive method for the determination of MDA, which had been assumed to be an indicator of lipid peroxidation.
(2) We made an apparatus to measure oxygen consumption, which was the most reliable index for the evaluation of lipid peroxidation. Based on the oxygen consumption, the efficiency and the limit of the parameters such as MDA, TBA-RS and tocopherols were in the autoxidation of soybean and rapeseed oils were investigated.
(3) Liver regeneration taking place after the intoxication of carbon tetrachloride which damaged the liver by extensive lipid peroxidations was found to be regulated by alpha_1-receptor, which controlled liver regeneration following partial hepatectomy.
(4) Ascorbic acid is known to be the most important anti-oxidant in biological systems. We established the method to determine the vitamin specifically and sensitively involving chemical derivatization and HPLC.
(5) As a chemical index of aging, we developed a sensitive and specific method to determine peroxides and hydroperoxides using chemiluminescence.
(6) The toxic mechanism of cadmium to the lung was investigated by evaluating the change in antioxidant system after the instillation of the metal into rat trachea. Based on the change of glutathione, it was found that cadmium caused lipid peroxidation.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] E.Kishida: "Determination of malondialdehyde with chemical derivatization into the pyrimidine compound and HPLC." Biochim.Biophys.Acta. 1045. 187-188 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Ochi: "Effect of α-blockade on liver regeneration after carbontertra-chloride intoxication in the rat." Biochem.Pharmacol.39. 2065-2066 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] E.Kishida: "Relationship among malondialdehyde,TBA-reactive substances and tocopherols in the oxidation of rapeseed oil." J.Nutr.Sci.Vitaminol.36. 619-623 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] E.Kishida: "Relationship among malondialdehyde,TBA-reactive substances and tocopherols in the oxidation of soybean oil." J.Home Econ.Jpn.42. 219-222 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Iguchi: "Early decreases in pulmonary,hepatic and renal glutathione levels in response to cadmium instillation into rat trachea." J.Appl.Toxicol.11. 211-217 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Kojo: "Peroxyoxalate chemiluminescent assay in aqueous solution using γ-cyclodextrin." Clin.Chem.(1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] E. Kishida, M. Oribe, K. Mochizuki, S. Kojo and H. Iguchi: "Determination of malondialdehyde with chemical derivatization into the pyrimidine compound and HPLC." Biochem. Biophys. Acta. 1045. 187-188 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y. Ochi, Y. Yumori, A. Morioka, K. Miura, I. Tsukamoto, S. Kojo: "Effect of alpha-blockade on liver regeneration after carbon tetrachloride intoxication in the rat." Biochem. Pharmacol.39. 2065-2066 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] E. Kishida, M. Oribe and S. Kojo: "Relationship among malondialdehyde, TBA-reactive substances and tocopherols in the oxidation of rapeseed oil." J. Nutr. Sci. Vitaminol.36. 619-623 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] E. Kishida, M. Oribe and S. Kojo: "Relationship among malondialdehyde, TBA-reactive Substances and tocopherols in the oxidation of soybean oil." J. Home Econ. Jpn.42. 219-222 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Iguchi, S. Kojo and M. Ikeda: "Early decrease in pulmonary, hepatic and renal glutathione levels in response to cadmium instillation into rat trachea." J. Appl. Toxicol.11. 211-217 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S. Kojo, S. tokumaru, E. Kishida and I. Tsukamoto: "Peroxyoxalate chemiluminescent assay in aqueous solution using gamma-cyclodextrin." Clin. Chem.(1992)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-16  

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