• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1991 Fiscal Year Final Research Report Summary

Enzyme immunoassay of human Mn-superoxide dismutase and its clinical application : Monitoring for myocardial injury.

Research Project

Project/Area Number 02557097
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 医学一般
Research InstitutionOsaka University

Principal Investigator

TANIGUCHI Naoyuki  Osaka Univ. Med. Sch. Professor, 医学部, 教授 (90002188)

Co-Investigator(Kenkyū-buntansha) UDA Taizou  Ube Res. Lab. Ube. Ind. Ltd. Researcher, 主任研究員
TADA Michihiko  Osaka Univ. Med. Sch. Professor, 医学部, 教授 (90093434)
Project Period (FY) 1990 – 1991
KeywordsSuperoxide Dismutase / Enzyme Immumoassay / Myocardial Injury / Acute Myocardial Infarction / Cardiac Function / Tumor Necrosis Factor
Research Abstract

An enzyme-linked immunosorbent assay (ELISA) has been developed for human manganese-superoxide dismutase (Mn-SOD), using a specific monoclonal antibody raised against the purified enzyme. The MN-SOD molecule comprises four identical sub-units this permitted the development of a symmetrical assay, using the same monoclonal antibody as both capture and detector. The assay offers a specific, sensitive and convenient means of measuring immunoreactive MN-SOD in human sera. Under optimum conditions, the sensitivily of the assay permits the detection of 2-200 ng of purified MN-SOD from human liver. The mean serum Mn-SOD levels of normal healthy males and females were 99.8 <plus-minus> 24.8. (mean <plus-minus> SD) and 88.8 <plus-minus> 20.8 (mean <plus-minus> SD), respectively. Serum MN-SOD levels were determined in patients with acute myocardial infarction by ELISA. The serial determinations performed on 29 patients with acute myocardial infarction indicated that MN-SOD appears in serum in a biphasic manner ; an early small elevation was observed at approximately 16.2 (<plus-minus> 7.3) h and a high elevation at apporoximately 108 (<plus-minus> 20.6) h after the onset of symptoms. A negative correlation was found between the maximum value of the late elevation of MN-SOD and left ventricul

  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Kawaguchi,T.: "Serum Mn-superoxide dismutase:Normal values and increased levels in patients with acute myocardial infarction and several malignant diseases determined by enzyme-linked immunosorbent assay using a monoclonal antibody." J.Immunol.Methods. 127. 249-254 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Matsuda,Y.: "Human liver manganese superoxide dismutase:Purification and crystallization,subunit association and sulfhydryl reactivity." Eur.J.Biochem.194. 713-720 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Deutsch,H.F.: "Preparation of human manganese superoxide dismutase by Tri-phase partitionig and preliminary crystallographic data." J.Mol.Biol.219. 103-108 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Suzuki,K.: "Elevation of immunoreactive serum Mn-superoxide dismutase in patients with acute myocardial infarction." Free Red.Res.Commun.(1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kawaguchi, T.: "Serum Mn-superoxide dismutase : Normal values and increased levels in patients with acute myocardial infarction and several malignant diseases determined by enzyme-linked immunosorbent assay using a monoclonal antibody." J. Immunol. Methods. 127. 249-254 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsuda, Y.: "Human liver manganese superoxide dismutase : Purification and crystallization, subunit association and sulfhydryl reactivity." Eur. J. Biochem.194. 713-720 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Deutsch, H. F.: "Preparation of human manganese superoxide dismutase by Tri-Phase partitioning and preliminary crystallographic data." J. Mol. Biol.219. 103-108 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suzuki, K.: "Elevation of immunoreactive serum Mn-superoxide dismutase in patients with acute myocardial infarction." Free Rad. Res. Commun.(1992)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1993-03-16  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi