1992 Fiscal Year Final Research Report Summary
TLC separation and fluorometric derivatization analysis of urinary polyamine and its application for diagnoses of cancer
Project/Area Number |
02640442
|
Research Category |
Grant-in-Aid for General Scientific Research (C)
|
Allocation Type | Single-year Grants |
Research Field |
分析・地球化学
|
Research Institution | Hamamatsu University School of Medicine |
Principal Investigator |
ICHINOSE Norio Hamamatsu University School of Medicine, Department of Chemistry, Professor, 医学部, 教授 (60035136)
|
Project Period (FY) |
1990 – 1992
|
Keywords | Polyamines / Urinary polyamines / TLC separation / fluorescent / diagnoses |
Research Abstract |
Polyamines of putrescine, cadaverine, spermidine, spermine and so on exist widely in nature of plant and animal world. Recently, it is recognized that the concentration of these polyamines in the blood and urine of a cancerous patient becomes higher than those of a normal person, therefore, a trend of the polyamines in the human body fluids has attracted special medical interest as a new tumor marker the formation of Schiff base derivatives from the reaction of the above-mentioned polyamines and several aromatic aldehydes and their fluorescent properties have studied, and then, 1-pyrenealdehyde was found to be suitable fluorogenic reagent for the methanol medium. Thus, the analyses of the Schiff base derivatives of various polyamines and 1-pyrenealdehyde using high-performance liquid chromatography (HPLC) equipped with fluorescence monitor and/or a thin layer chromatography (TLC) were examined. The Schiff base was formed at the surface of TLC plate after developing, then, fluorescent densitometry of developed TLC plate was carried out a dual wavelength flying spot scanner. Experimental Results The fluorescent intensity of the Schiff base's fluorophor of the various polyamines developping on a TLC plate lowered rapidly as the time proceeds due to evaporation of the developping solvent. Therefore, in order to the prevent this evaporation, much hard work has been done last year (1992). Consequently, it was successful that the holding time of the fluorescent intensity of polyamines fluorophors keeps for a limitted time by covering the fluorescent polyamine's spot on the TLC plate with a coating agent in an organic solvent. However, it has not been obtained a quantitative results yet. Moreover, I will continue this point since next year.
|
Research Products
(2 results)