1991 Fiscal Year Final Research Report Summary
A Study on Synthetic Method of Monodisperse Oligo- and Polypeptedes
Project/Area Number |
02650660
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Research Category |
Grant-in-Aid for General Scientific Research (C)
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Allocation Type | Single-year Grants |
Research Field |
高分子合成
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Research Institution | Gunma University |
Principal Investigator |
OZAKI Takuo Gunma University Faculty of Engineering Associate Professor, 工学部, 助教授 (90008524)
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Project Period (FY) |
1990 – 1991
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Keywords | Monodisperse Peptide / Oligopeptide / Peptide Synthesis |
Research Abstract |
In this study, we have attempted to synthesize some monodisperse model peptides containing ^<13>C and ^<15>N labeled amino acids in a peptide chain, applying the our DCC-ONSu activated estr method. This method has been reported in our previous paper. we have successfully synthesized some monodisperse oligopeptides(octadecamer and nonamer)containing L-alanine-1- ^<13>C and L-alanine-2- ^<13>C in a peptide chain of gamma-benzyl-L-gltanate by using this method, in order to clarify the local conformation, intra- and intermolecular interactions in the peptide chain. We have also synthesized a series of oligopeptids(hexamer)consisting of ^<15>N labeled L-alanine and L-leucne residues or ^<15>N labeled L-leucne and L-alanine residues. As a result, we have found that this method is sufficiently useful to synthesize the monodisperse compound. This method is much more useful for the synthesis of monodisperse oligopeptide containing the valuable ^<13>C and ^<15>N labeled amino acids rather than the solidphase synthetic method, because the number of the reaction steps is exceptionally small and a considerable amount of the final product can be obtained and so on. From our result, it should be anticipated that various monodisperse oligo- and polypeptides can be obtained by using this method on the basis of gamma-benzyl-L-gltamate. We are going to analyze the solid-state conformation of the samples obtained in this study by measuring ^<13>C and ^5N cross polarization-magic angle spinning(CPMAS)NMR spectra.
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