1991 Fiscal Year Final Research Report Summary
Moduration of tumor associated shedding antigen by irradiation and antitumor drugs.
Project/Area Number |
02670885
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Research Category |
Grant-in-Aid for General Scientific Research (C)
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Allocation Type | Single-year Grants |
Research Field |
補綴理工系歯学
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Research Institution | Hokkaido University |
Principal Investigator |
MIZUKOSHI Takanori Hokkaido Univ. Dental Hospital Lecture, 歯学部附属病院, 講師 (10113630)
|
Project Period (FY) |
1990 – 1991
|
Keywords | Radiation / Anti-tumor drug / Tumor associated antigen / Anti-tumor immunity |
Research Abstract |
We previously reported that rat fibrosarcoma KMT- 17 cell line and its subclone A3 shed a TAA, termed CE7, from the cell surface and that this phenomenon might play a role as an immunological escape mechanism. We have also reported that the anti-tumor immunity of KMT-17 bearing rats was enhanced by local 30Gy irradiation and this may lead to suppression of tumor metastases. In this study we examined effects of radiation and anti-tumor drug treatment on expression of the CE7 antigen. 1)Shedding of the CE7 was inhibited and the antigen was expressed on the cell surface by both radiation and anti-tumor drug treatment. 2)As a result of expression of the CE7 antigen on the cell surface, immunogenicity of the tumor cells was enhanced. These results suggest that the CE7 antigen expressing cells stimulate the host anti-tumor immunity. In order to clarify the mechanisms of this antigen shedding, we analyzed the CE7 antigen expression in vitro under several growth conditions. 1)Expression of the CE7 antigen was enhanced according to supression of cell growth. 2)The CE7 antigen exists in cytoplasm and culture media but not on the cell surface when the cell growth was stimulated. Our data suggest that expression of the CE7 antigen on the cell surface is associated with growth suppression of the tumor cells caused by irradiation or treatment of anti- tumor drug.
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Research Products
(4 results)