1992 Fiscal Year Final Research Report Summary
Development of a congenic strain on the mouse prion protein gene
| Project/Area Number |
02680039
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Laboratory animal science
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
HANDA Sumio Kyushu University, Faculty of Medicine, Associate professor, 医学部, 助教授 (50037503)
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| Co-Investigator(Kenkyū-buntansha) |
KITAMOTO Tetsuyuki Kyushu University, Faculty of Medicine, Assistant professor, 医学部, 講師 (20192560)
MOHRI Shirou Saga Medical School,Faculty of Medicine, Associate professor, 医学部, 助教授 (40117271)
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| Project Period (FY) |
1990 – 1992
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| Keywords | animal model / Creutzfeldt-Jakob disease(CJD) / prion disease / congenic mouse / SCID mouse / follicular dendritic cell(FDC) |
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| Research Abstract |
Congenic mouse concerning with prion protein (PrP) gene is an useful animal model for Creutzfeldt-Jakob disease (CJD) and other so-called "prion disease" of human and animals.
In order to establish a congenic mouse strain on the gene of PrP, we mated with two inbred strains of mice using back-cross method between NZW/Sea which has PrP-a allele and I/LnJ has PrP-b allele. PrP allelic analysis at DNA level was established and improved to select heterozygote out of the offspring. When the back-crossing generation was developed at 6th, the breeding was disordered. We have re-started new back-crossing from the beginning. So, it will take more time to establish a mouse congenic strain with PrP gene.
On the other hand, we found that C.B.17-scid(SCID) mouse has an unique susceptibility to prion disease, that is, the incubation period after intraperitoneal inoculation with CJD agent is more than 600 days whereas it is about 150 days after intracerebral inoculation, and no abnormal isoform PrP are detected in the follicular dendritic cells (FDCs) of spleen and lymph node.
These evidence suggest that FDCs may play a major role on the accumulation of abnormal PrP and the incubation period after intraperitoneal inoculation with CJD.
SCID mouse should be an useful model to clarify the mechanism of prion disease.
We are going to progress an animal model of prion disease to combine the congenic mouse with SCID mouse.
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