1993 Fiscal Year Final Research Report Summary
Molecular Anatomy on Intercellular Junctions
| Project/Area Number |
03404015
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| Research Category |
Grant-in-Aid for General Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | National Institute for Physiological Sciences |
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Principal Investigator |
TSUKITA Shigenobu National Institute for Physiol. Sci. Prof., 生理学研究所, 教授 (50155347)
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| Co-Investigator(Kenkyū-buntansha) |
NAGAFUCHI Akira National Institute for Physiol. Sci. Assistant Prof., 生理学研究所, 助手 (80218023)
TSUKITA Sachiko National Institute for Physiol. Sci. Assistant Prof., 生理学研究所, 助手 (00188517)
YONEMURA Shigenobu National Institute for Physiol. Sci. Assistant Prof., 生理学研究所, 助手 (60192811)
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| Project Period (FY) |
1991 – 1993
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| Keywords | cell adhesion / adherens junctions / radixin / undercoat / adhesion apparatus / tyrosin phosporylation / tyrosin kinase / monoclonal antibody |
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| Research Abstract |
The ERM family members, ezrin, radixin, and moesin, localizing just beneath the plasma membranes are thought to be involved in the actin filament/plasma membrane association. To identify the intergral membrane protein directly associated with ERM family members, we performed immunoprecipitation studies using anti-moesin mAb and cultured BHK cells metabolically-labeled with [^<35>S]metionine or surface-labeled with biotin. The results indicated that moesin is directly associated with a 140kD integral membrane protein. Using BHK cells as antigens, we obtained a mAb which recognized the 140kD membrane protein. We next cloned a cDNA encoding the 140kD membrane protein and identified it as CD44, a broadly-distributed cell surface glycoprotein. Immunoprecipitation with various anti-CD44 mAbs showed that ezrin and radixin as well as moesin are associated with CD44 not only in BHK cells but also in mouse L fibroblasts. Furthermore, immunofluorescence microscopy revealed that in both BHK and L cells, the Triton X-100-insoluble CD44 is precisely colocalized with ERM family members. We concluded that ERM family members work as molecular linkers between the cytoplasmic domain of CD44 and actin-based cytoskeletons.
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[Publications] Sato, N., Yonemura, S., Obinata, T., Tsukita, S., and Tsukita, S: "Radixin, a barbed-end-capping actin-modulating protein, is concentrated at the cleavage furrow during cytokinesis" J.Cell Biol.113. 321-330 (1991)
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「研究成果報告書概要(欧文)」より
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[Publications] Tsukita, S., Oishi, K., Akiyama, T., Yamanashi, Y, Yamamoto, T., and Tsukita, S.: "Spcecific proto-oncogenic tyrosine kinases of src family are enriched in cell-to-cell adherens junctions where the level of tyrosine phosphorylation is elevated" J.Cell Biol.113. 867-879 (1991)
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「研究成果報告書概要(欧文)」より
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[Publications] Yonemura, S., Nagafuchi, A., Sato, N., and Tsukita, S.: "Concentration of an integral membrane protein, CD43 (leukosialin, sialophorin), in the cleavage furrow through the interaction of its cytoplasmic domain with actin-based cytoskeleton" J.Cell Biol.120. 437-449 (1993)
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「研究成果報告書概要(欧文)」より
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[Publications] Itoh, M., Nagafuchi, A., Yonemura, S., Yasuda-Kitani, T., Tsukita, S., and Tsukita, S.: "The 220kD protein colocalizing with cadherins in non-epithelial cells is identical to ZO-1, a tight junction-associated protein in epithelial cells : cDNA cloning and immunoelectron microscopy" J.Cell Biol.121. 491-502 (1993)
Description
「研究成果報告書概要(欧文)」より
