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1992 Fiscal Year Final Research Report Summary

Functional Morphology of A PUD neurons in the central nervous system

Research Project

Project/Area Number 03670027
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 神経解剖学
Research InstitutionKyoto prefectural University of Medicine

Principal Investigator

OKAMURA Hitoshi  Kyoto Pref. Univ. Med., Pept. Anatomy, Associate Professor, 医学部, 助教授 (60158813)

Co-Investigator(Kenkyū-buntansha) TAGUCHI Junichi  Kyoto Pref Univ. Med., Pept. Anatomy, Assistant, 医学部, 助手 (50188132)
Project Period (FY) 1991 – 1992
KeywordsAmine Precursor uptake and decarboxylation / L-DOPA / Aromatic L-amino acid decarboxylase / dopamine / ^3H-DOPA / Hypothalamus / Brain
Research Abstract

For synthesis of catecholamines, two synthetic enzymes are at least needed. One is tyrosine hydroxylase (TH), which converts tyrosine to L-DOPA, and another is aromatic L-amino acid decarboxylase (AADC), which converts L-DOPA to dopamine. It has been demonstrated that there is a group of neurons containing AADC but lacks TH in the brain, but its functional roles is still unclear. In the present study, we investigated the possibility that these central AADC(+)/TH(-) neurons have the ability to "amine precursor uptake and decarboxylation (APUD). I. Distribution of APUD cells in the rat central nervous system Male Wistar rats were sacrificed 60 min after loading of L-DOPA (20 mg/kg, i.p.). They were processed for double labeling immunocytochemistry using mouse monoclonal anti-dopamine serum and rabbit anti-AADC serum. All the AADC-immunoreactive cells in the brain showed dopamine-immunoreactivity. This suggest that non-aminergic AADC neurons have ability to convert amine precursor to amines (APUD-activity). II. Projection of cenral APUD cells Gold labeled choleratoxin-B, a retrograde neuronal tracer, were injected into the hippocampus, amygdala and septum, and into various region of the hypothalamus. These rats were processed for AADC or TH immunocytochemistry. These investigations have revealed supramammillary nonaminergic AADC neurons project to the hippocampus, but other non-aminergic AADC neurons innervate only in the hypothalamus. only inside the hypothalamus. III. Uptake of [3H]DOPA After 60 min of [3H]DOPA injection, we performed in vivo autoradiography. High density of isotope-labeling was detected in the caudate-putamen, nucleus accumbens, and tuberculum olfactorium. Hypothalamus showed weak but distinct labeling. This suggest that hypothalamus, a region rich in non-aminergic AADC neurons and fibers, have lower ability for uptaking DOPA than that of caudate-putamen, in which abundant nigrostriatal dopaminergic endings are found.

  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Ichitani,Y.,Okamura,H.,et.al.: "Degeneration of the nigral dopamine neurons after 6-hydroxydopamine injection into the rat striatum." Brain Research. 549. 350-353 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tanaka,M.,Okamura,H.,et.al.: "L-dopa-immunoreactivity in hypothalamic magnocellular neurons of hypothalamo-neurohypophysial system." Neuroscience Research. 16. S29- (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamada,K.,Okamura,H.,et.al.: "Detection of tyrosine hydroxylase and phenylet hanolamine-N-methyl trnaferase messenger RNAs in the mouse adrenal gland and the brain by in situ hybridization." Histochemistry. 97. 201-206 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Okamura,H.,Tanaka,M.,et.al.: "A simple combined technique of histofluorescence and peptide-immunocytochemistry for the analysis of the coexistence of monoamine and peptide in brain stem neurons." Biogenic Amines. 9. 195-204 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Matsumoto,Y.,Okamura,H.,et.al.: "Immunocytochemical survey of haloperidol-induced immunoreactive changes of[Met]enkephalin-Arg6-Gly7-Leu8 in the rat forebeain." Brain Research Bulletin. 28. 683-695 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 岡村 均: "L-DOPA含有ニューロンの形態学" 医学のあゆみ. 162. 823-826 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 岡村 均: "組織細胞化学1992" 学際企画, 174 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Okamura, H., Kitahara, K., Mons, N., Matsumoto, Y., Ibata, Y. and Geffard, M.: "Heterogenous distribution of L-dopa immunoreactivity in dopaminergic neurons of the rat midbrain." Basic, Clinical, and therapeutic Aspect of Alzheimers and Parkinsons Disease. Vol.1 (Nagatsu, T. et. al. eds). 423-426 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ichitani, Y., Okamura, H., Matsumoto, Y., Nagatsu, I. and Ibata, Y.: "Degeneration of the nigral dopamine neurons after 6-hydroxydopamine injection into the rat striatum." Brain Res.549. 350-353 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okamura, H., Yokoyama, C., Taguchi, J., and Ibata, Y.: "Distribution of dopamine D2 receptor in the rat brain : autoradiographic analysis using [3H] YMO9151-2." Neurosci. Res.Suppl., 16. S20 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tanaka, M., Okamura, H., Kitahama, K., Kawakami, F., Mons, N., Gefferd, M. and Ibata, Y.: "L-dopa-immunoreactivity in hypothalamic magnocellular neurons of hypothalamo-neurohypophysial system." Neurosci. Res.Suppl., 16. S29 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yokoyama, C., Okamura, H. and Ibata, Y.: "Self-mutilation behavior and brain dopamine in neonatal 6-hydroxydopamine treated rats." Neurosci. Res.Suppl., 16. S147 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamada, K., Sakai, M., Okamura, H., Ibata, Y. and Nagatsu, I: "Detection of tyrosine hydroxylase and phenylethanolamine-N-methyl trnaferase messenger RNAs in the mouse adrenal gland and the brain by in situ hybridization." Histochemistry. 97. 201-206 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Uno, T., Hisa, Y., Murakami, Y., Okamura, H. and Ibata, Y.: "Distribution of tyrosine hydroxylase immunoreactive fibers in the canine larynx." Eur. Arch. Otorhinolaryngol.249. 40-43 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okamura, H., Tanaka, M., Hisa, Y., Nagatsu, I. and Ibata, Y.: "A simple combined technique of histofluorescence and peptide-immunocytochemistry for the analysis of the coexistence of monoamine and peptide in brain stem neurons." Biogenic Anmines. 9. 195-204 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsumoto, Y., Okamura, H., Ichitani, Y., Yanaihara, N., Nakajima, T. and Ibata, Y.: "Immunocytochemical survey of haloperidol-induced immunoreactive changes of [Met] enkephalin-Arg6-Gly7-Leu8 in the rat forebrain." Brain Res. Bull.28. 683-695 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tanaka, M., Okamura, H., Yanaihara, N., Tanaka, Y. and Ibata, Y.: "Differential expression of serotonin and [Met] enkephalin-Arg6-Gly7-Leu8 in neurons of the rat brain stem." Brain Res. Bull.(1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yokoyama, C., Okamura, H., and IBATA, Y.: "Resistance of hypothalamic dopaminergic neurons to neonatal 6-hydroxydopamine toxicity." Brain Res. Bull.(1993)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1994-03-24  

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