1993 Fiscal Year Final Research Report Summary
Analysis of cancer-associated carbohydrate antigen in human colon cancer -aim at clinical utilities-
Project/Area Number |
03670639
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Research Category |
Grant-in-Aid for General Scientific Research (C)
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Allocation Type | Single-year Grants |
Research Field |
Digestive surgery
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Research Institution | OSAKA CITY UNIVERSITY |
Principal Investigator |
OKUNO Masahiro Osaka city University, First Department of Surgery, Associate Professor, 医学部, 助教授 (10047144)
|
Project Period (FY) |
1991 – 1993
|
Keywords | human colorectal cancer / glycosphingolipid antigen / Le^b antigen / two-dimensional thin-layr chromatography / mass spectrometry / human colon cancer cell line, OCUC-M, OCUC-F |
Research Abstract |
The expression of cancer associated glycosphingolipid antigens was examined in colorectal cancer and adjacent normal mucosa, obtained by operation. The glycosphingolipids were isolated from the tissue speciments and analyzed by two-dimensional thin-layr chromatography, and were compared to those in normal mucosa in the same patients. The structure of glycosphingolipid antigen was determined by mass spectrometry of permethylated reduced sugars and negative ion FAB/MS analysis of the intact glycolipid, and TLC-immunostaining with monoclonal anti-Le^a, -Le^b, -Le^x, and -Le^y antibody. As the results, a novel neutral glycosphingolipid appeared in colorectal cancer was identified to be the III^4 IV^2-Fuc2Lc4Cer, as Lewis^b antigen. The ceramide was composed mainly of 2-hydroxy C24 fatty accid and C18 phytosphingosine. In such individuals, the expression of Le^b antigen in normal mucosa is abscent or minimal. Furthermore, the increase of CDH and the decrease of CMH was found in cancer tissues. These were considered to be cancer-associated changes in distal colorectal tissue. Two clonal human colon cancer cell line, OCUC-M, OCUC-F were established from pleural effusion of a patient with colon cancer and characterized. These cell line may be useful in study of growth and differentiation, and heterogeneity fo cancer cells.
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Research Products
(6 results)