1992 Fiscal Year Final Research Report Summary
RECOVERY FROM A LETHAL INFECTION WITH HSV : ROLE OF gamma deltaT CELLS AND ANALYSIS OF ORAL DEFENSE MECHANISM
| Project/Area Number |
03670843
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Morphological basic dentistry
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| Research Institution | NIPPON DENTAL UNIVERSITY (1992)
Tohoku University (1991) |
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Principal Investigator |
SHIMIZU Fumio NIPPON DENTAL UNIVERSITY PROFESSOR, 歯学部, 教授 (10162710)
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| Co-Investigator(Kenkyū-buntansha) |
SUZUKI Susumu TOHOKU UNIVERSITY ASSISTANT PROFESSOR, 歯学部, 助手 (70216399)
MONMA Yuko TOHOKU UNIVERSITY ASSISTANT PROFESSOR, 歯学部, 助手 (00191073)
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| Project Period (FY) |
1991 – 1992
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| Keywords | Gingiva / HSV type 1 / Infection / Intraperitoneal cavity / TcRgamma delta cells / Vbeta11^+alphabetacells / Virulence |
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| Research Abstract |
We focused our interest on the pathogenesis of virulent Miyama GC+ strain of herpes simplex virus type 1 (HSV-GC^+) in different routes of inoculation in a mouse model system. Results are as follows.
1. When Balb/C mice were infected with 10^2 plaque-forming unit(PFU) of HSV-GC^+ intraperitoneally, one hundred percent of mice were dead. HSV was recovered from the organs such as brainstem, spleen, and liver. Mice were, however, infected in maxillary gingiva with 10^7 PFU(10^4 lethal dose _<50>) of HSV, all of mice were survived. HSV was recovered from trigeminal ganglia, but not from other organs tested.
2. It was shown that preinoculation of HSV via maxillary gingiva of Balb/C mice confer-red complete protection against HSV challenge via peritoneal cavity.
3. When Balb/C mice were infected in maxillary gingiva with HSV and followed by intra-peritoneal injection of cyclophosphamide 72 hr after virus inoculation, one hundred % of mice were dead. HSV were recovered from brain tissue. These results indicate that nonlethal infection in maxillary gingiva with HSV was converted into a lethal infection in such immunosuppressed mice.
4. When mice were infected in maxillary gingiva with HSV-GC+, there can be detected twice as many TcRgamma delta cells as noninfected mice.
These data suggest that there can be in oral cavity the host defense mechanism against HSV infection, which is different from in peritoneal cavity. This mechanism may exist in mice , regardless of the antigenesity of M1-2. It is worth studying the function of TcRgamma delta cells which have the important role in the eary stage of infection.
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