1992 Fiscal Year Final Research Report Summary
Estimation of plasma dopamine concentration and its clinical significance
| Project/Area Number |
03671111
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Laboratory medicine
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
YOSHIMURA Manabu Kyoto Prefectural University of Medicine, Faculty of Med, Professor, 医学部, 教授 (40094453)
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| Project Period (FY) |
1991 – 1992
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| Keywords | dopamine / catecholamine / HPLC / exercise / hypertension / conjective heart failure / parkinsonisms / laboratory medicine |
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| Research Abstract |
The majority of circulating catecholamines (CAs) is in a sulfoconjugated form in human plasma and the plasma free dopamine (DA) concentration was the detection limit of other high-performance liquid chromatography (HPLC) techniques. Using diphenylethylenediamine derivatives for fluorogenic reagent, it is possible to estimate free DA concentration as little as 0.03 pmol/ml in HPLC analysis. The valves of free DA obtained from healthy volunteers were 6.4 * 1.5pg/ml in young subjects and 16.2 * 8.2pg/ml in aged subjects, which indicated the effect of age on plasma DA concentration. Plasma DA level increased in sitting and standing position. During 24 hours, plasma DA level did not change. During exercise, plasma DA level increased with the enhanced intensity of exercise and showed the positive correlation with plasma lactic acid level, indicating vasodilating effects on the vessels at muscle and skin. Plasma DA concentration increased in patients with pheochromocytoma, primary aldorterorism, congestive heart failure and L-DOPA treatment of parkinsonisms. The estimation of plasma DA concentration can be applied for differential diagnosis in laboratory medicine
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