1993 Fiscal Year Annual Research Report

エイズ制圧に向けての基礎研究・エイズの動物モデル

Research Project

Project/Area Number	04269103
Research Institution	Kyoto University
Principal Investigator	速水正憲京都大学, ウイルス研究所, 教授 (40072946)
Co-Investigator(Kenkyū-buntansha)	岩倉洋一郎東京大学, 医科学研究所, 教授 (10089120) 等泰道東京大学, 医科学研究所, 助手 (10222241) 見上彪東京大学, 農学部, 教授 (20091506) 向井鐐三郎予研筑波霊長類センター, 主任研究官 (90133040)
Keywords	ネコ免疫不全ウイルス / サル免疫不全ウイルス / マウスエイズ / トランスジェニックマウス / エイズ
Research Abstract	SHIV:実験用サルに感染・発症させるHIV-1の作成を目的として、新たにSIVmacとのキメラウイルス6株を作成し、その1株をカニクイ・アカゲザルに接種したところ、感染効率がよく、今後の発症が期待される(速水)。 SIV:新たに入手したSIVmac株をアカゲザル9頭に接種したところ、1頭で神経異常を認め死亡した。エイズ脳症のモデルと期待され、脳から分離されたウイルスの解析を行っている(向井)。 FIV:FIV遺伝子のORF-AとLTR内のAP-1結合部位のウイルス増殖への関与、及びネコ細胞におけるRev遺伝子機能、FIV遺伝子発現へのヘルペスウイルス遺伝子の関与を明らかにした。このようにネコエイズを起こすFIVの遺伝子機能の理解が進んだ(見上)。マウスエイズ:IFN-γとIL-10とが、免疫応答不全に関与すること及び非受容体型チロシンキナーゼ(Btk)が関与するB細胞が発症に重要であることが示唆された。現在その解析を進めている(等)。 HIVトランスジェニックマウス:全遺伝子をもつマウスへのLPSもしくはSEBなどのスーパー抗原の投与により、TNF-αやIL-6発現の亢進に伴いHIVの発現が上昇した。またヒトCD4導入マウスと掛け合わせたところ、CD4細胞が減少した。このことはこのマウスが未発達キャリアーのモデルとなりうることを示す(岩倉)。

Research Products

(26 results)

All Other

All Publications (26 results)

[Publications] Tomonaga,K.: "Isolation and characterization of simian immunodeficiency virus from African white-crowned mangabey monkey(Cercocebus torquatus lunulatus)" Arch.Virol.129. 77-92 (1993)
[Publications] Igarashi,T.: "Analysis of long-term carrier states of macaque monkeys infected with HIV-1/SIVmac chimeric viruses." Proceeding of IXth International Conference on AIDS(Berline). 1. 26 (1993)
[Publications] 佐多徹太郎: "サル免疫不全ウイルス実験感染アカゲザルのリンパ節病変の推移" Medical Immunology. 26. 581-584 (1993)
[Publications] Miyazawa,T.: "The AP-1 binding site in the feline immunodeficiency virus long terminal repeat is not required for virus replication in feline T lymphocytes" J.Gen.Virol.74. 1573-1580 (1993)
[Publications] Miyazawa,T.: "Biological nature of feline immunodeficiency virus" J.Vet.Med.Sci.55. 519-526 (1993)
[Publications] Momoi,Y.: "Detection of feline immunodeficiency virus proviral DNA in feline perpiheral blood mononuclear cells by the nested two-step polymerase chain reaction" Zbl.Bakt.279. 274-282 (1993)
[Publications] Norimine,J.: "Feline CD4 molecules expressed on feline non-lymphoid cell lines are not enough for productive in-fection of highly lymphotropic feline immunodeficiency virus isolates" Arch.Virol.130. 171-178 (1993)
[Publications] Tomonaga,K.: "Feline immunodeficiency virus gene expression analysis of the RNA splicing pattern and the monocistronic rev mRNA." J.Gen.Virol.74. 2409-2417 (1993)
[Publications] Tomonaga,K.: "The feline immunodeficiency virus ORF-A gene facilitates efficient virus replication in established T cell lines and perpheral blood lymphocytes" J.Virol.67. 5889-5895 (1993)
[Publications] Toyosaki,T.: "Localization of the Viral antigen of feline immunodeficiency virus in the lymph nodes of cats at the early stage of infection" Arch.Virol.131. 335-347 (1993)
[Publications] Hitoshi,Y.: "Delayed progression of a murine retro-virus-induced acquired immunodeficiency Syndrom,MAIDS,in X-linked immunodeficient mice" J.Exp.Med.177. 621-626 (1993)
[Publications] Hitoshi,Y.: "Interleukin 5 receptor positive B cells,but not eosinophils,are functionary and numerically influenced in the mice carrying the X-linked immune defect" Int.Immunol.5. 1183-1190 (1993)
[Publications] Katoh,S.: "Maintenance of CD5+Bcells at an early developmental stage by interleukin-5 transgenic mice." DNA AND CELL BIOLOGY. 12. 481-491 (1993)
[Publications] Yamamoto,H.: "Inflammatory polyarthritis in mice transgenic for human T-cell leukemia virus type1." Arth.Rheum. 36. 1612-1620 (1993)
[Publications] Iwakura,Y.: "Transgenic mice carrying interferon genes" Mol.Repl.Devel.36. 245-247 (1993)
[Publications] Nagashima,H.: "Aberrant tissue specific expression of the transgene in transgenic mice that carry the hepatitis B virus genome defective in the X gene" Arch.Virol.132. 381-397 (1993)
[Publications] Endo,T.: "Structural changes in the N-linked sugar chains of serum immunoglobulin G of HTLV-1 transgenic mice" Biochem.Biophys.Res.Commun.192. 1004-1010 (1993)
[Publications] Karaki,S.: "HLA-B51 transgenic mice as recipients for production of polymorphic HLA-A,-B-specific antibodies" Immun Genet.37. 139-142 (1993)
[Publications] Iwakura,Y.: "Mechanisms of HIV gene activation in transgenic mice carrying the HIV genome" IXth International Conference on AIDS(Berline). 1. 26 (1993)
[Publications] 速水正憲: "霊長類レトロウイルスの分子進化「レトロウイルス疾患」" 病理と臨床(文光堂), 7 (1993)
[Publications] 速水正憲: "人とサルの免疫不全ウイルスからみたエイズの起源" 医学のあゆみ, 3 (1993)
[Publications] 速水正憲: "実験用サルに感染するエイズウイルス(HIV-1)作成法の開発" 現代免疫学のテクノロジー(医学書院), 11 (1993)
[Publications] 速水正憲: "実験用サルに感染するヒト免疫不全ウイルス1型(HIV-1)" 感染・炎症・免疫, 8 (1993)
[Publications] 向井鐐三郎: "AIDS発症アカゲザルの日和見感染" 感染・炎症・免疫, 3 (1993)
[Publications] 吉川泰弘: "AIDSの動物モデル" Mebio, 11 (1993)
[Publications] Iwakura,Y.: "Introduction of foreign genes into germ line cells of animals:Production of viral disease models." Japan Scientific Societies Pres-Karger,Tokyo, 4 (1993)

1993 Fiscal Year Annual Research Report

エイズ制圧に向けての基礎研究・エイズの動物モデル

Principal Investigator

速水 正憲 京都大学, ウイルス研究所, 教授 (40072946)

Research Products

[Publications] Tomonaga,K.: "Isolation and characterization of simian immunodeficiency virus from African white-crowned mangabey monkey(Cercocebus torquatus lunulatus)" Arch.Virol.129. 77-92 (1993)

[Publications] Igarashi,T.: "Analysis of long-term carrier states of macaque monkeys infected with HIV-1/SIVmac chimeric viruses." Proceeding of IXth International Conference on AIDS(Berline). 1. 26 (1993)

[Publications] 佐多徹太郎: "サル免疫不全ウイルス実験感染アカゲザルのリンパ節病変の推移" Medical Immunology. 26. 581-584 (1993)

[Publications] Miyazawa,T.: "The AP-1 binding site in the feline immunodeficiency virus long terminal repeat is not required for virus replication in feline T lymphocytes" J.Gen.Virol.74. 1573-1580 (1993)

[Publications] Miyazawa,T.: "Biological nature of feline immunodeficiency virus" J.Vet.Med.Sci.55. 519-526 (1993)

[Publications] Momoi,Y.: "Detection of feline immunodeficiency virus proviral DNA in feline perpiheral blood mononuclear cells by the nested two-step polymerase chain reaction" Zbl.Bakt.279. 274-282 (1993)

[Publications] Norimine,J.: "Feline CD4 molecules expressed on feline non-lymphoid cell lines are not enough for productive in-fection of highly lymphotropic feline immunodeficiency virus isolates" Arch.Virol.130. 171-178 (1993)

[Publications] Tomonaga,K.: "Feline immunodeficiency virus gene expression analysis of the RNA splicing pattern and the monocistronic rev mRNA." J.Gen.Virol.74. 2409-2417 (1993)

[Publications] Tomonaga,K.: "The feline immunodeficiency virus ORF-A gene facilitates efficient virus replication in established T cell lines and perpheral blood lymphocytes" J.Virol.67. 5889-5895 (1993)

[Publications] Toyosaki,T.: "Localization of the Viral antigen of feline immunodeficiency virus in the lymph nodes of cats at the early stage of infection" Arch.Virol.131. 335-347 (1993)

[Publications] Hitoshi,Y.: "Delayed progression of a murine retro-virus-induced acquired immunodeficiency Syndrom,MAIDS,in X-linked immunodeficient mice" J.Exp.Med.177. 621-626 (1993)

[Publications] Hitoshi,Y.: "Interleukin 5 receptor positive B cells,but not eosinophils,are functionary and numerically influenced in the mice carrying the X-linked immune defect" Int.Immunol.5. 1183-1190 (1993)

[Publications] Katoh,S.: "Maintenance of CD5+Bcells at an early developmental stage by interleukin-5 transgenic mice." DNA AND CELL BIOLOGY. 12. 481-491 (1993)

[Publications] Yamamoto,H.: "Inflammatory polyarthritis in mice transgenic for human T-cell leukemia virus type1." Arth.Rheum. 36. 1612-1620 (1993)

[Publications] Iwakura,Y.: "Transgenic mice carrying interferon genes" Mol.Repl.Devel.36. 245-247 (1993)

[Publications] Nagashima,H.: "Aberrant tissue specific expression of the transgene in transgenic mice that carry the hepatitis B virus genome defective in the X gene" Arch.Virol.132. 381-397 (1993)

[Publications] Endo,T.: "Structural changes in the N-linked sugar chains of serum immunoglobulin G of HTLV-1 transgenic mice" Biochem.Biophys.Res.Commun.192. 1004-1010 (1993)

[Publications] Karaki,S.: "HLA-B51 transgenic mice as recipients for production of polymorphic HLA-A,-B-specific antibodies" Immun Genet.37. 139-142 (1993)

[Publications] Iwakura,Y.: "Mechanisms of HIV gene activation in transgenic mice carrying the HIV genome" IXth International Conference on AIDS(Berline). 1. 26 (1993)

[Publications] 速水正憲: "霊長類レトロウイルスの分子進化「レトロウイルス疾患」" 病理と臨床(文光堂), 7 (1993)

[Publications] 速水正憲: "人とサルの免疫不全ウイルスからみたエイズの起源" 医学のあゆみ, 3 (1993)

[Publications] 速水正憲: "実験用サルに感染するエイズウイルス(HIV-1)作成法の開発" 現代免疫学のテクノロジー(医学書院), 11 (1993)

[Publications] 速水正憲: "実験用サルに感染するヒト免疫不全ウイルス1型(HIV-1)" 感染・炎症・免疫, 8 (1993)

[Publications] 向井鐐三郎: "AIDS発症アカゲザルの日和見感染" 感染・炎症・免疫, 3 (1993)

[Publications] 吉川泰弘: "AIDSの動物モデル" Mebio, 11 (1993)

[Publications] Iwakura,Y.: "Introduction of foreign genes into germ line cells of animals:Production of viral disease models." Japan Scientific Societies Pres-Karger,Tokyo, 4 (1993)

速水正憲京都大学, ウイルス研究所, 教授 (40072946)