1993 Fiscal Year Annual Research Report

RNA機能構造の新視点

Research Project

Project/Area Number	04272103
Research Institution	The University of Tokyo
Principal Investigator	横山茂之東京大学, 大学院・理学系研究科, 教授 (00159229)
Co-Investigator(Kenkyū-buntansha)	河合剛太東京大学, 工学部, 助手 (70211860) 岡田典弘東京工業大学, 生命理工学部, 教授 (60132982) 武藤〓弘前大学, 理学部, 教授 (80034635) 渡辺公綱東京大学, 工学部, 教授 (00134502) 志村令郎京都大学, 理学部, 教授 (60025426)
Keywords	RNA / 機能構造 / NMR / 高次構造 / mRNAスプライシング / tRNA / rRNA / RNA触媒(リボザイム)
Research Abstract	本重点領域研究「RNA機能構造の新視点」は,RNA機能の発現機構について,従来とは異なる新しい視点(高次構造レベルの解析など)から,機能するRNAの実像を捉えることを目的としている.重点領域研究の2年度にあたる平成5年度においては,総括班は設定した3つの研究項目に関して,3つの計画研究班および21の公募研究班を総括し,昨年度につづき順調に成果をあげている.本領域研究の中心課題であるmRNAのスプライシングに関しては,エキソン中に存在するスプライシングを促進する配列を発見した.一方,スプライシング反応のモデル系を構築することにも成功し,その反応のメカニズムを解析している.tRNAについては,異常構造をとるミトコンドリアtRNAについて,試験管内の反応によって大量の分子を取得することに成功した.これらのそれぞれについて,昨年度に総括班で設置した最新の高次構造解析システム(600MHz高分解能NMR分光計およびデータ解析用コンピュータ)を用いた高次構造解析に着手しており,成果をあげつつある.これらの解析には,昨年度に整備した高分子量RNAのNMR解析に必須の安定同位体標識のための原料供給システムが効果的に機能している.一方,スプライシングを調節するタンパク質のRNA結合部位については,高次構造解析システムを用いて立体構造を決定することに成功した.この他にも多くの重要な成果が得られている.このように,本重点領域研究の特色である,分子生物学から高次構造解析まで幅広く異なる方法論をもつ研究者の間での緊密な連携が,総括班の指導のもとに計画研究および公募研究の双方にわたって効果的に行われ,計画以上の成果をあげている.

Research Products

(16 results)

All Other

All Publications (16 results)

[Publications] Sakamoto,K.: "A modified uridine in the first position of the anticodon of a minor species of arginie tRNA,the argU gene product,from Escherichia coli" Eur.J.Biochem.216. 369-375 (1993)
[Publications] Sekine,M.: "Synthesis of Uridylyl (3'-5') Uridine Derivatives Containing 5-(methylamino-methyl) uridine as a Modified Nucleoside Found From E.Coli Minor tRNA^<Arg>" Nucleosides & Nucleotides. 12. 305-321 (1993)
[Publications] Muto,Y.: "Sequence-Specific ^1H and ^<15>N Resonance Asignments and Secondary Structures of GDP-bound human c-Ha-Ras Protein in Solution" J.Biomol.NMR. 3. 165-184 (1993)
[Publications] Nureki,O.: "Chemical Modification and Mutagenesis Studies on Zinc Binding of Aminoacyl-tRNA Synthetases" J.Biol.Chem.268. 16368-16373 (1993)
[Publications] Watanabe,A.: "The Role of Exon Sequence in Splice Site Selection" Gene & Development. 7. 407-418 (1993)
[Publications] Inagaki,Y.: "Lack of Peptide-Release activity Responding to Codon UGA in Mycoplasma Capricolum" Nucleic Acids Res.21. 1335-1338 (1993)
[Publications] Kano,A.: "Unassigned pr Nonsense Codons in Micrococcus luteus" J.Mol.Biol.230. 51-56 (1993)
[Publications] Ohama,T.: "Non-universal decording of the leucine codon CUG in several Candida species" Nucleic Acids Res.21. 4039-4045 (1993)
[Publications] Asahara,H.: "Recognition nucleotides of Escherichia coli tRNA^<Leu> and its elements facilitating discrimination from tRNA^<Ser> and tRNA^<Tyr>" J.Mol.Biol.231. 219-229 (1993)
[Publications] Suzuki,T.: "The gene for serine tRNA having anticodon sequence CAG in pathogenic yeast,Candida albicans" Nucleic Acids Res.21. 356 (1993)
[Publications] Uchida,C.: "A Small RNA of Mycoplasma capricolum that Resembles Eukaryotic U6 ^<sn> RNA" Nucleic Acids Res.21. 2649-2653 (1993)
[Publications] Kojima,S.: "Close Phylogenetic Relationship between Vestimentifera (tube worms) and Annelida Revealed by the Amino Acid Sequence of Elongation Factor-1α" J.Mol.Evol.37. 66-70 (1993)
[Publications] Yoshioka,Y.: "Determination of the Phylogenetic Relationships among Pacific salmonide using Short Interspersed Elements (SINEs) as Temporal Landmarks of Evolution" Proc.Natl.Acad.Sci.U.S.A.90. 6995-6999 (1993)
[Publications] Ohshima,K.: "Molecular Characterization of a Short Interspersed Repetitive Element (SINE) from tabaco that Exhibits Sequence Homology to Specific tRNAs" Proc.Natl.Acad.Sci.U.S.A.90. 6562-6566 (1993)
[Publications] Ogawa,K.: "Anticodon loop structure of Trulopsis utilis tRNA^<Val> and valine acceptance" J.Adv.Sci.5. 43-49 (1993)
[Publications] Ohyama,T.: "ATP and Mg^<2+> inos regulate both conformation and aminoacylation capacity of transfer RNA" J.Adv.Sci.5. 39-42 (1993)

1993 Fiscal Year Annual Research Report

RNA機能構造の新視点

Principal Investigator

横山 茂之 東京大学, 大学院・理学系研究科, 教授 (00159229)

Research Products

[Publications] Sakamoto,K.: "A modified uridine in the first position of the anticodon of a minor species of arginie tRNA,the argU gene product,from Escherichia coli" Eur.J.Biochem.216. 369-375 (1993)

[Publications] Sekine,M.: "Synthesis of Uridylyl (3'-5') Uridine Derivatives Containing 5-(methylamino-methyl) uridine as a Modified Nucleoside Found From E.Coli Minor tRNA^<Arg>" Nucleosides & Nucleotides. 12. 305-321 (1993)

[Publications] Muto,Y.: "Sequence-Specific ^1H and ^<15>N Resonance Asignments and Secondary Structures of GDP-bound human c-Ha-Ras Protein in Solution" J.Biomol.NMR. 3. 165-184 (1993)

[Publications] Nureki,O.: "Chemical Modification and Mutagenesis Studies on Zinc Binding of Aminoacyl-tRNA Synthetases" J.Biol.Chem.268. 16368-16373 (1993)

[Publications] Watanabe,A.: "The Role of Exon Sequence in Splice Site Selection" Gene & Development. 7. 407-418 (1993)

[Publications] Inagaki,Y.: "Lack of Peptide-Release activity Responding to Codon UGA in Mycoplasma Capricolum" Nucleic Acids Res.21. 1335-1338 (1993)

[Publications] Kano,A.: "Unassigned pr Nonsense Codons in Micrococcus luteus" J.Mol.Biol.230. 51-56 (1993)

[Publications] Ohama,T.: "Non-universal decording of the leucine codon CUG in several Candida species" Nucleic Acids Res.21. 4039-4045 (1993)

[Publications] Asahara,H.: "Recognition nucleotides of Escherichia coli tRNA^<Leu> and its elements facilitating discrimination from tRNA^<Ser> and tRNA^<Tyr>" J.Mol.Biol.231. 219-229 (1993)

[Publications] Suzuki,T.: "The gene for serine tRNA having anticodon sequence CAG in pathogenic yeast,Candida albicans" Nucleic Acids Res.21. 356 (1993)

[Publications] Uchida,C.: "A Small RNA of Mycoplasma capricolum that Resembles Eukaryotic U6 ^<sn> RNA" Nucleic Acids Res.21. 2649-2653 (1993)

[Publications] Kojima,S.: "Close Phylogenetic Relationship between Vestimentifera (tube worms) and Annelida Revealed by the Amino Acid Sequence of Elongation Factor-1α" J.Mol.Evol.37. 66-70 (1993)

[Publications] Yoshioka,Y.: "Determination of the Phylogenetic Relationships among Pacific salmonide using Short Interspersed Elements (SINEs) as Temporal Landmarks of Evolution" Proc.Natl.Acad.Sci.U.S.A.90. 6995-6999 (1993)

[Publications] Ohshima,K.: "Molecular Characterization of a Short Interspersed Repetitive Element (SINE) from tabaco that Exhibits Sequence Homology to Specific tRNAs" Proc.Natl.Acad.Sci.U.S.A.90. 6562-6566 (1993)

[Publications] Ogawa,K.: "Anticodon loop structure of Trulopsis utilis tRNA^<Val> and valine acceptance" J.Adv.Sci.5. 43-49 (1993)

[Publications] Ohyama,T.: "ATP and Mg^<2+> inos regulate both conformation and aminoacylation capacity of transfer RNA" J.Adv.Sci.5. 39-42 (1993)

横山茂之東京大学, 大学院・理学系研究科, 教授 (00159229)