NEW FLUOROGENIC SYNTHETIC SUBSTRATES AND INHIBITORS FOR MICROSOMAL METALLOENDOPEPTIDASE

1993 Fiscal Year Final Research Report Summary

• Project/Area Number: 04558025
• Research Category: Grant-in-Aid for Developmental Scientific Research (B)
• Allocation Type: Single-year Grants
• Research Field: 物質生物化学
• Research Institution: KYUSHU UNIVERSITY
• Principal Investigator: KAWABATA Shun-ichiro KYUSHU UNIVERSITY, FACULTY OF SCIENCE, RESEARCH ASSOCIATE, 理学部, 助手 (90183037)
• Co-Investigator(Kenkyū-buntansha): NISHINO Norikazu KYUSHU INSTITUTE OF TECHNOLOGY, FACULTY OF ENGINEERING, PROFESSOR, 工学部, 教授 (40145165) ; IWANAGA Sadaaki KYUSHU UNIVERSITY, FACULTY OF SCIENCE, PROFESSOR, 理学部, 教授 (90029942)
• Project Period (FY): 1992 – 1993
• Keywords: Processing protease / Metalloendopeptidase / Synthetic substrate / Fluorogenic substrate / Metalloprotease

Research Abstract

Ten fluorogenic synthetic substrates of Abz (2-aminobenzoyl)-peptide-Dna(2,4-dinitro-anilinoethylamide) were newly synthesized and tested to find specific substrates for rabbit liver microsomal metalloendopeptidase (MEP) as shown in Table. Among these substrates, Abz-ARVRRANS-Dna or Abz-ARVTRANS-Dna was found to be the most sensitive substrates for MEP.The assay method using these peptide substrates enabled us to detect MEP activity in an aliquot (20-50 mul) of the crude extract.

Research Products

• [Publications] Kawabata,S.: "A microsomal endopeptidase from liver with substrate specificity for processing proproteins such as the vitamin K-dependent proteins of plasma." The Journal of Biological Chemistry. 267. 10331-10336 (1992)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Kawabata,S.: "Rabbit liver microsomal endopeptidase with substrate specificety for processing proproteins is structurally related to rat testes metalloendopeptidase 24.15." The Journal of Biological Chemistry. 268. 12498-12503 (1993)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Ikura,T.: "Secondary Structural Features of Modules M2 and M3 of Barnase in Solution by NMR Experiment and Distance Geometry Calculation." Proteins. 16. 341-356 (1993)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Sakumi,K.: "Cloning and Expression of cDNA for a Human Enzyme That Hydrolyzes 8-Oxo-dGTP,a Mutagenic Substrate for DNA Synthesis." The Journal of Biological Chemistry. 268. 23524-23530 (1993)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Miura,Y.: "A Limulus Intracelluar Coagulation Inhibitor with Characteristics of the Serpin Superfamily,Purification,Characterization,and cDNA Cloning." The Journal of Biological Chemistry. 269. 542-547 (1994)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Kawabata, S.and Davie, E.W.: "A microsomal endopeptidase from liver with substrate specificity for processing proproteins such as the vitamin K-dependent proteins of plasma." J.Biol.Chem.267. 10331-10336 (1992)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Kawabata, S., Nakagawa K., Muta, T., Iwanaga, S., and Davie, E.W.: "Rabbit liver microsomal endopeptidase with substrate specificety for processing proproteins is structurally related to rat testes metalloendopeptidase 24.15" J.Biol.Chem.268. 12498-42503 (1993)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Ikura, T., Go, N., Kohda, D., Inagaki, F., Yanagawa, H., Kawabata, M., Kawabata, S., Iwanaga, S., Noguti, T., and Go. M.: "Secondary Structural Features of Modules M2 and M3 of Barnase in Solution by NMR Experiment and Distance Geometry Calculation" Proteins. 16. 341-356 (1993)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Sakumi, K., Furuichi, M., Tsuzuki, T., Kakuma, T., Kawabata, S., Maki, H., and Sekiguchi, M.: "Cloning and Expression of cDNA for a Human Enzyme That Hydrolyzes 8-Oxo-dGTP, a Mutagenic Substrate for DNA Synthesis." J.Biol.Chem.268. 23524-23530 (1993)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Miura, Y., Kawabata, S., and Iwanaga, S.: "A Limulus Intracelluar Coagulation Inhibitor with Characteristics of the Serpin Superfamily, Purification, Characterization, and cDNA Cloning." J.Biol.Chem.269. 542-547 (1994)
  • Description: 「研究成果報告書概要(欧文)」より