1993 Fiscal Year Final Research Report Summary
Analysis of the erythroid-specific gene expression by GATA trenscription factors
Project/Area Number |
04670157
|
Research Category |
Grant-in-Aid for General Scientific Research (C)
|
Allocation Type | Single-year Grants |
Research Field |
Pathological medical chemistry
|
Research Institution | Tohoku University |
Principal Investigator |
YAMAMOTO Masayuki Tohoku University, Biochemistry, Lecturer, 医学部, 講師 (50166823)
|
Project Period (FY) |
1992 – 1993
|
Keywords | GATA factors / Erythroid cells / Cell defferentiation / Testis / Sertoli cells |
Research Abstract |
To understand functional roles that GATA factors play during erythroid cell differentiation, we examined the expression of GATA factors at both mRNA and protein levels. We also examined the structure of the genes encoding GATA factors. The results of expression analysis of GATA factors in leukemia-derived cell lines suggest that GATA-1 is essential for the development of erythroid/megakaryocytic cells, whereas GATA-2 is important for much earlier stage of cells in hematopoietic lineage. To define which GATA factor actually binds to a particular GATA sequence in vivo, we isolated DNA/GATA-1 complex from nuclei of mouse erythroleukemia cells by using anti-GATA-1 monoclonal antibody. We found genes which are expressed specifically in erythroid lineage cells in the immunoprecipitate. In contrast, we could not find genes which have GATA sites but are expressed in cells different from erythroid lineage. This finding indicates that GATA-1 binds only to the GATA sequences in the regulatory region for erythroid genes and suggests that GATA-1 is important for erytyroid gene expression. We found that GATA-1 is also expressed in mouse testis. Whereas the primary structure of this factor is completely the same as that in erythroid cells, the expression of testis form GATA-1 mRNA was found to be driven by the testis-specific promoter. We determined human GATA-2 and chicken GATA-3 genes, and found that the organization of these genes are very well conserved between species and between each GATA factor. Based on these results, we concluded that GATA factors function as regulators of thissue-specific gene expression.
|
Research Products
(29 results)
-
-
-
-
-
-
-
-
-
-
-
-
[Publications] Mitani, K., Fujita, H., Hayashi, N., Yamamoto, M., Sassa, S.: "Defferential induction responses of *delta<[Greek]>-aminolevulinate synthase mRNAs during erythroid differentiation" Hematol, Am. J.39. 63-64 (1992)
Description
「研究成果報告書概要(欧文)」より
-
-
[Publications] Ito, E., Toki, T., Ishihara, H., Ohtani, H., Gu, L., Yokoyama, M., Engel, J.D., Yamamoto, M.: "Erythroid transcription factor GATA-1 is transcribed abundantly in mouse testis" Nature. 362. 466-468 (1993)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Konishi, K., Takata, Y., Yamamoto, M., Yomogida, K., Watanabe, K., Tsurufuji, S., Fujioka, M.: "Structure of the gene encoding rat neutrophil chemo-attractant Gro." Gene. 126. 285-286 (1993)
Description
「研究成果報告書概要(欧文)」より
-
-
[Publications] Komatsu, N., Yamamoto, M., Fujita, H., Miwa, A., Hatake, K., Endo, T., Okano, H., Katsube, T., Fukumaki, Y., Sassa, S., Miura, Y.: "Establishment and characterization of an erythropoetin-dependent subline, UT-7/Epo derived from human leukemia cell line, UT-7" Blood. 82. 456-465 (1993)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Nakahashi, Y., Miyazaki, H., Kadota, Y., Naito, Y., Inoue, K., Yamamoto, M., Hayashi, N., Taketani, S.: "Molecular defect in human erythropoietic protoporphyria with fatal liver failure" Human Genet.91. 303-306 (1993)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Shiga, K., Shiga, C., Sasano, N., Miyazaki, S., *Yamamomo, T., Yamamoto, M., Hayashi, N., Nishihira, T.Mori, S.: "Expression of c-erbB2 in human esophageal carcinoma cells : overexpression correlated with gene amplification or with GATA-3 transcription factor expression" Anticancer Res.13. 1293-1302 (1993)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Nakahashi, Y., Miyazaki, H., Kadota, Y., Naito, Y., Inoue, K., Yamamoto, M.Hayashi, N., Taketani, S.: "Human erythropoietic protoporphyria : identification of a mutation at the splice donor site of intron7 causing exon7 skipping of the ferrochelatase gene" Human Mol. Gene. 2. 1069-1070 (1993)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Endo, K., Harigae, H., Nagai, T., Fujie, H., Meguro, K., Watanabe, N., Furuyama, K., Kameoka, J., Okuda, M., Yamamoto, M., Abe, K.: "Two chronic myelogenous cell lines which represent different stages of erytyroid differentiation" Br. J.Hematrol.85. 653-662 (1993)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Chiba, T., Nagata, Y., Kishi, A., Sakamaki, K., Miyajima, A., Yamamoto, M., Engel, J.D., and Todokoro, K.: "Induction of erythroi-specific gene expression in lymphoid cells" Proc. Natl. Acad. Sci. U.S.A.90. 11593-11597 (1993)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Konishi, K., Takata, Y., Watanabe, K., Date, T., Yamamoto, M., Murase, A., Yoshida, H., Suzuki, T., Tsurufuji, S., Fujioka, M.: "Cytokine" Cytokine. 5. 506-511 (1993)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Yatsunami, K., Ohtsu, H., Tsuchikawa, M., Higuchi, T., Ishibashi, K., Shiba, A., Shima, Y., Nakagawa, S., Yamauchi, K., Yamamoto, M., Hayashi, N.Watanabe, T., Ichikawa, A.: "Structure of the L-histidine decarboxylase gene" J.Biol. Chem.269. 1554-1559 (1994)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Igarashi, K., Kataoka, K., Itoh, K., Hayashi, N., Nishizawa, M., Yamamoto, M.: "Regulation of transcription by demerization of erythroid transcription factor NF-E2 p45 with small Maf proteins" Nature. 367. 568-572 (1994)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Yang, Z., Gu, L., Bories, D., Romeo, P.-H., Motohashi, H., Yamamoto, M., Engel, J.D.: "Human GTA-3 trans-activation, DNA binding and nuclear localization activities are organized into distinct structural domains" Mol. Cell. Biol.14. 2201-2212 (1994)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Yomogida, K., Ohtani, H., Harigae, H., Ito, E., Nishimune, Y., Engel, J.D., Yamamoto, M.: "Developmental stage- and spermatogenic cycle- dependent expression of GATA-1 in mouse Sertoli cells" Development. (in press). (1994)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Kornhause, J., *Leoard, M.W., Yamamoto, M., LaVail, J.H., Mayo, K.E., Engel, J.D.: "Temporal and spacial changes in TATA-transcription factor expression coincide with development of the chicken optic tectum" Mol. Brain Res.(in press). (1994)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Lim, K.-C., Ishihara, H., Riddle, R.D., Yang, Z., Andrews, N., Orkin, S.H., Yamamoto, M., Engel, J.D.: "Structure and tissue-specific expression of the chicken erythroid *delta-aminolevulinate synthase gene" Nucleic Acdis Res.(in press). (1994)
Description
「研究成果報告書概要(欧文)」より