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1993 Fiscal Year Final Research Report Summary

Enzymochemical Study on growth of Psychrotrophs during Food Preservation in Refrigirator

Research Project

Project/Area Number 04680070
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 家政学
Research InstitutionNara Women's University

Principal Investigator

YOKOIGAWA Kumio  Nara Women's Univ. Faculty of Human Life and Environment, Assistant Prof., 生活環境学部, 講師 (60230637)

Co-Investigator(Kenkyū-buntansha) KAWAI Hiroyasu  Nara Women's Univ. Faculty of Human Life and Environment, Prof., 生活環境学部, 教授 (80026525)
Project Period (FY) 1992 – 1993
KeywordsFood preservation / Psychrotroph / Food deterioration / Alanine racemase
Research Abstract

Alanine racemase essential to growth of bacteria was purified from psychrotrophs isolated from foods. The enzymesfrom typical psychrotrophs, Pseudomonas fluorescens, bacillus psychrophilus and Bacillus psychrosaccharolyticus were markedly inactivated when incubated at over 30゚C for 1 h, and the bacteria could not growat over 30゚C.Thus, the region of growth temperature were consistent with the thermostability of the enzymes. Since the amino acid composition and sequence were similar to those of other alanine racemases from mesophiles and a thermophile, alanine racemase may be suitable for comparative study on thermostability. The enzymes from psychrotrophs were thermolabile, and inactivated by incubation with denaturants andorganic solvents. Especially, 20% ethanol markedly inactivated the thermolabile enzymes even at 0゚C.Ethanol was effective as a sterilizer to psychrotrophs.

  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] Young-Hee Lim: "A new amino acid racemase with threonine α-epimerase activity from Pseudomonas putida:purification and characterization." J.Bacteriology. 175. 4213-4217 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kumio Yokoigawa: "Thermolabile alanine racemase from a psychrotroph,Pseudomonas fluorescens:purification and properties." Biosci.Biotech.Biochem.57. 93-97 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Young-Hee Lim: "A new amino acid racemase with threonine alpha-epimerase activity from Pseudomonas putida : purification and characterization" J.Bacteriology. 175. 4213-4217 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kumio Yokoigawa: "Thermolabile alanine racemase from a psychrotroph, Pseudomonas fluorescens : purification and properties" Biosci. Biotech. Biochem.57. 93-97 (1993)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1995-03-27  

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