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1995 Fiscal Year Final Research Report Summary

Basic and clinical studies on Paracoccidioidomycosis

Research Project

Project/Area Number 05045044
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionUniversity-to-University Cooperative Research
Research InstitutionChiba University

Principal Investigator

MIYAJI Makoto  Research Center of Pathogenic Fungi and Microbial Toxicoses Chiba University, Professor, 真核微生物研究センター, 教授 (40009494)

Co-Investigator(Kenkyū-buntansha) BAGAGLI Edusado  Department of Medicine in Botucutu campus, UNESP Professor, ボツカツ校医学部, 教授
MARQUES Silv  パウリスタ総合大学, ボツカツ校医学部, 教授
MENDES Rinal  パウリスタ総合大学, ボツカツ校医学部, 教授
MONTENEGRO M  パウリスタ総合大学, ボツカツ校医学部, 教授
FRANCO Marcello  Department of Medicine in Botucutu campus Universidade Estadual Paulista (UNESP), ボツカツ校医学部, 教授
YOSHIDA Soichi  Research Center of Pathogenic Fungi and Microbial Toxicoses' Chiba University, R, 真核微生物研究センター, 助手 (20114340)
TAKEO Kanji  Research Center of Pathogenic Fungi and Microbial Toxicoses Chiba University, Pr, 真核微生物研究センター, 教授 (10027097)
FUKUSHIMA Kazutaka  Research Center of Pathogenic Fungi and Microbial Toxicoses' Chiba University, A, 真核微生物研究センター, 助教授 (90114321)
NISHIMURA Kazuko  Research Center of Pathogenic Fungi and Microbial Toxicoses Chiba University, Pr, 真核微生物研究センター, 教授 (00114314)
MARQUES Silvio Alencar  Department of Medicine in Botucutu campus, UNESP Professor
MENDES Rinaldo Ponceo  Department of Medicine in Botucutu campus, UNESP Professor
MONTENEGRO Mario R.G.  Department of Medicine in Botucutu campus, UNESP Professor
Project Period (FY) 1993 – 1995
KeywordsBrazil / Paracoccidioides brasiliensis / Paracoccidioidomycosis / Ecology / Imported mycosis / Pathogenic dematiaceous fungi / Dimorphic fungus
Research Abstract

Paracoccidioidomycosis, caused by the thermal dimorphic fungus Paracoccidioides brasiliensis, is systemic human mycosis geographically confined to Latin America. The natural habitat of P.brasiliensis is still unclear. Since 1993 to study this mycosis we have done University-to-University cooperative Research with Botucutu Campus of Universidade Estadual Paulista (UNESP), Brasil. The university hospital of UNESP in Botucutu receives patients with paracoccidioidomycosis from the endemic area. Because a culture medium with high plating efficiency for P.brasiliensis is of critical importance for isolation of the fungus from natural environments, efforts have been made to improve the plating efficiency of culture medium and we found that when a water extract of yeast phase cells P.brasilienses was added in combination withhorse serum to brain heart infusion agar (BHIA), the plating efficiency was highly improved. Using this medium we tried to isolate the causative agent from nature. About 800 samples of soil from various areas were gathered and cultured. Among them five yeast like colonies which resembled P.brasiliensis were obtained. Microscopically these five isolates formed chlamydospores on SDA and converted from mycelial to yeast at 35 C on BHIA.Multiple budding were also seen. We also obtained ninety-eight black colonies from isolation of pathogenic dematiaceous fungi. They were Curvularia lunata, Exophiala spinifera, exophiala spp, Fonsecaea pedrosoi, Phialophora verrucosa, Ramichloridium anceps, Rhinocladiella atrovirens and Veronea botyosa. 581 strains of Aspergillus spp. were isolated from 887 samples collected.

  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] Y.Horie: "New and interesting species of Neosartorya from Brazilian soil" Mycoscience. 36. 199-204 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Taguchi: "Studies on the synergistic effect of amphotericin B and 5-fluorocytosi on the growth rate of single hyphae....." Mycoscience. 36. 341-344 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] N.Kurita: "Growth-promoting factors for yeast cells of Paracoccidioides brasiliensis" Mycoscience. (発表予定).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A.Sano: "Ecological study of possibiy pathogenic fungi found in nature in Botucutu,Soa Paulo,Brazil,an endemic area of......" Mycopathologia. (発表予定).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 宮治 誠: "人に棲みつくカビの話" 草思社, 197 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Horie: "New and interesting species of Neosartorya from Brazilian soil" Mycoscience. 36. 199-204 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Taguchi: "Studies on the synergistic effect of amphotericin B and 5-fluorocytosine on the growth rate of single hyphae of Apergillus fumigatus by a biocell-tracer system" Mycoscience. 36. 341-344 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] N.Kurita: "Growth-promoting factors for yeast cells of Paracoccidioides brasiliensis" Mycoscience.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A.Sano: "Ecological study of possibly pathogenic fungi found in nature in Botucatu, Sao Paulo, Brazil, an endemic area of Paracoccidioidomycosis" Mycopathologia.

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1997-03-04  

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