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1996 Fiscal Year Final Research Report Summary

Interfacial Electron Transfer of Metalloproteins -Design of Functional Electrode Surfaces and Its Application in Bioelectrochemistry-

Research Project

Project/Area Number 06045042
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionUniversity-to-University Cooperative Research
Research InstitutionKumamoto University

Principal Investigator

TANIGUCHI Isao  Faculty of Engineering, Kumamoto University, Professor, 工学部, 教授 (90112391)

Co-Investigator(Kenkyū-buntansha) FARRELL Nich  バージニアコモンウエルス大学, 化学科, 準教授
CHELEBOWSKI Jan F  Department of Biochemistry, Virginia Commonwealth University, 化学科, 教授
HAWKRIDGE Fred M  Department of Chemistry, Virginia Commonwealth University, 化学科, 教授
KIDA Kenji  Faculty of Engineering, Kumamoto University, 工学部, 教授 (00195306)
NISHIYAMA Katsuhiko  Faculty of Engineering, Kumamoto University, 工学部, 講師 (10202243)
FARRELL Nicholas P  Department of Chemistry, Virginia Commonwealth University
Project Period (FY) 1994 – 1996
KeywordsMetalloprotein / Interfacial electron transfer / Single crystal / Functional electrode / Spectroelectrochemical technique / Biocatalytic reaction / Circular dichroism / Digital simulation
Research Abstract

In the present study, electron transfer reactions of metalloproteins at functional electrodes have been examined to understand biofunctions of metalloproteins and to develop various bioelectrochemical reactions.
1. Interfacial electron transfer reactions of metalloproteins and their bioelectrochemical applications :
(1) Functional electrodes for rapid electron transfer of metalloproteins have been developed. (2) At the highly hydrophilic indium oxide electrode, some artificially modified myoglobin molecules have been studied. (3) For cytochrome c, clear effect of the structure of the modifier adsorbed onto gold single crystal surfaces on the electrochemical responses was observed. (4) Amino acid residues of ferredoxin have clearly shown to have their own distinguished roles for biological functions, such as controlling the redox potential and the complex formation with enzymes.
2. Dynamics of structural change of metalloproteins :
(1) Stopped-flow circular dichroism (CD) spectroelectrochem … More ical techniques has been developed. (2) Both cytochrome c and ferredoxin changed their structures by steps during electron transfer, and (3) some kinetic data of structural changes were obtained. (4) A fine structural change as a function of temperature was suggested for ferredoxin.
3. Application of ferredoxin electrochemistry to develop artificial photosynthetic reactions :
(1) Electrochemically reduced ferredoxin was applicable as an electron transfer mediator to develop new bioelectrocatalytic reactions as in nature. For example, L-malic acid was obtained with carbon dioxide fixation in the presence of ferredoxin-NADP^+-reductase and malic enzyme. L-glutamic acid was also obtained from oxoglutaric acid with by introducing of ammonia, and (2) their reactions were analyzed by using a digital simulation technique.
4. Semi-artificially designed metalloproteins :
(1) New biological functions have been found using semi-artificially reconstituted myoglobin molecules. (2) Computer calculation of the structure of ferredoxin showed that the modification of Ser-46 to Gly-46 introduced rather large distortion of the iron sulfur cluster, causing a large positive shift in redox potential. Less

  • Research Products

    (36 results)

All Other

All Publications (36 results)

  • [Publications] TANIGUCHI,Isao: "In Situ Circular Dichroism Spectroelectrochemistry of Spinach Ferredoxin Using a Promoter Modified Indium Oxide Electrode" Denki Kagaku. 62. 985-986 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] NISHIYAMA,Katsuhiko: "Kinetics of Confromational Change in Cyanomyoglobin Following Electron Trasfer Monitored by Magnetically Induced Circular Dichroism Spectroscopy" Biochem.Biophys.Res.Commun.205. 1724-1728 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] HAWKRIDGE,Fred M.: "The Direct Electron Transfer Reactions of Cytochrome c at Electrode Surfaces" Comments on Inorg.Chem.17. 163-187 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 谷口,功: "金属タンパク質の直接電子移動制御のための機能分子修飾電極デザイン" 化学と工業. 47. 1441-1443 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] ISHIYAMA,Munetaka: "Novel Disulfonated Tetrazolium Salt That can be Reduced to a Water-solubule Formazan and its Application to the Assay of Lactate Dehydrogenase" Analyst(London). 120. 113-116 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] TANIGUCHI,Isao: "Effect of Structure of the Redox Center on Electron Transfer Reaction of Myoglobin" Rev.of Polarography. 41. 87 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] TANIGUCHI,Isao: "Electrochemistry of Chlorella Ferredoxin at Modified Indium Oxide Electrodes and Its Application of Bioelectrocatalytic Reactions" Proc.2nd Japan-Korea Seminar on Electrochemistry. 11-12 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] TANIGUCHI,Isao: "Electrochemistry of Metalloproteins at Functional Electrodes" Proc.Int.Symp.on Electron Transfer in Proteins and Supramolecular Assemblies at Interfaces. 149-150 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] TANIGUCHI,Isao: "Preparation of Semi-Artificial Myoglobins and Their Electrochemical Reactions" Proc.7th Int.Symp.on Electrochemistry. 36-37 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] TANIGUCHI,Isao: "Electrochemistry of monoazahemin reconstituted myoglobin at an indium oxide electrode" J.Electroanal.Chem.(in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] TANIGUCHI,Isao: "Effect of the Structure of Modifiers Adsorbed on Gold Single Crystal Surfaces on the Promotion of the Electrode Reaction of Cytochrome c" Chemistry Letters. 1997(in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] FUSHINUKI,Yoshito: "Electrochemicl Analysis of Water-soluble Paraquat and Diquat in Urine" Denki Kagaku. 65(in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 谷口,功: "二酸化炭素-化学、生化学、環境-(現代化学 増刊25)" 東京化学同人, 70-78(192) (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] TANIGUCHI,Isao: "Novel trends in Electroorganic Syntesis" Kohdansha-Elsevier, 393-396(444) (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] TANIGUCHI,Isao: "Novel trends in Electroorganic Syntesis" Kohdansha-Elsevier, 399-400(444) (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 谷口,功: "詳説無機化学(第5章 酸化還元)" 講談社サイエンティフィク, 96-104(292) (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 谷口,功: "機器分析入門(第10章 電気分析)" 南江堂, 255-269(404) (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 谷口,功: "触媒技術の動向と展望:1997(研究動向:生体触媒)" 触媒学会(印刷中), (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] TANIGUCHI,Isao: "In Situ Circular Dichroism Spectroelectrochemistry of Spinach Ferredoxin Using a Promoter Modified Indium Oxide Electrode" Denki Kagaku. 62. 985-986 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] NISHIYAMA,Katsuhiko: "Kinetics of Confromational Change in Cyanomyoglobin Following Electron Trasfer Monitored by Magnetically Induced Circular Dichroism Spectroscopy" Biochem.Biophys.Res.Commun.205. 1724-1728 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] HAWKRIDGE,Fred M.: "The Direct Electron Transfer Reactions of Cytochrome c at Electrode Surfaces" Comments on Inorg.Chem.17. 163-187 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] TANIGUCHI,Isao: "Design of Functional Electrodes for Direct Electron Transfer of Metalloproteins (in Japanese)" Chemistry and Industry. 47. 1441-1443 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] ISHIYAMA,Munetaka: "Novel Disulfonated Tetrazolium Salt That can be Reduced to a Water-solubule Formazan and its Application to the Assay of Lactate Dehydrogenase" Analyst (London). 120. 113-116 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] TANIGUCHI,Isao: "Effect of Structure of the Redox Center on Electron Transfer Reaction of Myoglobin" Rev.of Polarography. 41. 87 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] TANIGUCHI,Isao: "Electrochemistry of Chlorella Ferredoxin at Modified Indium Oxide Electrodes and Its Application of Bioelectrocatalytic Reactions" Proc.2nd Japan-Korea Seminar on Electrochemistry. 11-12 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] TANIGUCHI,Isao: "Electrochemistry of Metalloproteins at Functional Electrodes" Proc.Int.Symp.on Electron Transfer in Proteins and Supramolecular Assemblies at Interfaces. 149-150 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] TANIGUCHI,Isao: "Preparation of Semi-Artificial Myoglobins and Their Electrochemical Reactions" Proc.7th Int.Symp.on Electrochemistry. 36-37 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] TANIGUCHI,Isao: "Preparation of Semi-Artificial Myoglobins and Their Electrochemical Reactions" J.Electroanal.Chem.(in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] TANIGUCHI,Isao: "Effect of the Structure of Modifiers Adsorbed on Gold Single Crystal Surfaces on the Promotion of the Electrode Reaction of Cytochrome c" Chemistry Letters. (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] TANIGUCHI,Isao: "Electrochemical Analysis of Water-soluble Paraquat and Diquat in Urine" Denki Kagaku. (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] TANIGUCHI,Isao: Tokyo Kagaku Dojin. Carbon Dioxide-Chemistry, Biochemistry, Environment-, 192 (70-78) (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] TANIGUCHI,Isao: Kohdansha-Elsevier. Novel Trends in Electroorganic Synthesis, 444 (393-396) (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] TANIGUCHI,Isao: Kohdansha-Elsevier. Novel trends in Electroorganic Synthesis, 444 (399-400) (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] TANIGUCHI,Isao: Kohdansha-Scientific. Inorganic Chemistry (Chap.5 : Oxidation-Reduction) (in Japanese), 292 (96-104) (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] TANIGUCHI,Isao: Nanko-do. Instrumental Analysis (Chap.10 : Electroanalysis) (in Japanese), 404 (255-269) (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] TANIGUCHI,Isao: Catalysis Soc.Japan (in press). Developments and Perspectives in Catalysis : (Biocatalysis) (in Japanese), (1997)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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