1996 Fiscal Year Final Research Report Summary
Identification of receptors for insect neuropeptides
| Project/Area Number |
06454081
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Bioproduction chemistry/Bioorganic chemistry
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
KATAOKA Hiroshi The University of Tokyo, Graduate School of Agriculture & Life Sciences, Associate Professor, 大学院・農学生命科学研究科, 助教授 (60202008)
|
|---|
| Project Period (FY) |
1994 – 1996
|
| Keywords | insect / rceptor / Bombyxin / insulin / PTTH / Bombyx |
|---|
| Research Abstract |
1. An Bombyx cell line, named BM-N4, has a single class of receptors for bombyxin with a Kd=2.4nM and each cell has 16,000 binding sites on the cell surface. The bombyxin receptor was composed of a several subunits linked with disulfide bonds and the molecular weight of binding subunit was about 105kDa. In addition, an about 92kDa protein was phosphorylated on tyrosine residue immediately after adding bombyxin.
2. The nucleotide sequence of the cDNA for bombyxin receptor from BM-N4 cells was elucidated by using PCR assisted method. The deduced amino acid seqence revealed that bombyxin receptor is similar to insulin receptor and composed of two subunits.
3. The prothoracic glands have a single class of receptors for PTTH with a Kd=54 pM and each cell has 16,000 binding sites on the cell surface. The molecular weight of PTTH receptor showed about 75kDa by cross-linking experiment.
|
