1995 Fiscal Year Final Research Report Summary
Molecular Biological Analysis of Invasion and Metastasis in Urological Cancer.
| Project/Area Number |
06454458
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Urology
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| Research Institution | The University of Tokushima |
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Principal Investigator |
KAGAWA Susumu University of Tokushima, School of Medicine Chief Professor, 医学部, 教授 (40035738)
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| Co-Investigator(Kenkyū-buntansha) |
NARUO Seiichi University of Tokushima, School of Medicine Assistant, 医学部, 助手 (20237621)
KANAYAMA Hiroomi University of Tokushima, University Hospital, Assistant Professor School of Medi, 医学部・附属病院, 講師 (10214446)
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| Project Period (FY) |
1994 – 1995
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| Keywords | RENAL CELL CARCINOMA / BLADDER CANCER / GENE EXPRESSION / TUMOR INVASION / TUMOR METASTASIS / MATRIX METALLOPROTEINASE / TISSUE INIHIBITOR OF METALLOPROTEINASE / CD44 |
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| Research Abstract |
[Purpose] Invasions and metastasis-related factors in human bladder cancer and renal cell carcinoma were evaluated by various methods using molecular biological techniques.
[Materials and Methods] Gene expression of matrix metalloproteinases (MMPs) and tissue inhibitor of metalloproteinases (TIMPs) was determined in 22 bladder cancer tissues by Northern blot and slot blot analyzes. The localization of MMP-2 and TIMP-2 was evaluated immunohistochemically. To determine the correlation with the clinicopathological features, serum levels of interleukin-1b (IL-1b), interleukin-6 (IL-6) and tumor necrosis-a (TNF-a) were measured in 32 renal cancer patients and expression of CD44 variant forms was analyzed in 19 renal cancer tissues by the reverse transcriptasepolymerase chain reaction (RT-PCR) method.
[Results] The mRNA level of MMP-2, TIMP-2 and ratio of MMP-2/TIMP-2 expression were correlated with the pathological stage (p<0.05, p<0.05, p<0.05) and tumor progression (p<0.005, p=0.058, p<0.005). Immunohistochemically, MMP-2 was lacalized on the cell surface of invasive tumor cells and TIMP-2 was localized around the invasive tumor focus. In renal cancer, elevated serum IL-6 level and enhanced expression of alternative splice variant forms of CD44 involving the exon v10 were correlated with tumor progression and rapid growth of renal cell carcinoma.
[Conclusion] In bladder cancer, expression of MMP-2 and TIMP-2 was correlated with tumor invasion and progression. In renal cancer, serum levels of IL-6 and expression of CD44 variant forms sharing exon v10 was correlated with tumor progression and rapid growth.
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