1995 Fiscal Year Final Research Report Summary
Development of highly efficient homologous recombination system using mouse recombination genes and establishment of mouse strains with gene engineered
Project/Area Number |
06454717
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Research Category |
Grant-in-Aid for General Scientific Research (B)
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Allocation Type | Single-year Grants |
Research Field |
Laboratory animal science
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Research Institution | Osaka University |
Principal Investigator |
MORITA Takashi Research Institute for Microbial Diseases, Osaka University, Department of Molecular Embryology, Associated Professor, 微生物病研究所, 助教授 (70150349)
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Project Period (FY) |
1994 – 1995
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Keywords | highly efficient recombination system / Rad51 gene / RecA gene / cell cycle / DNA repair / gene targeting / Dmc1 gene / meiosis |
Research Abstract |
The mouse Rad51 gene is a mammalian homologue of the Escherichia coli recA and yeast RAD51 genes, both of which are involved in homologous recombination and DNA repair in mitosis and meiosis. The expression of mouse Rad51 mRNA was examined in cell-cycle synchronized mouse m5S cells. The Rad51 transcript was observed from late G1 phase through to M phase. During the period of late G1-S-G2 phase, the RAD51 proteins were exclusively observed in nuclei. Activation of T cell and B cell proliferation in spleen by mitogens induced the expression of Rad51 mRNA.By immunohistochemical analyzes, the mouse RAD51 protein was detected in proliferating cells ; spermatogonia in testis, immature T cells in thymus, germinal center cells of the secondary lymphatic nodules of spleen and intestine, follicle cells in ovary and epithelial cells in uterus and intestine. It was also expressed in spermatocytes during the early and mid prophase of meiosis and in resting oocytes before maturation. Thus mouse Rad51 expression is closely related to the state of cell proliferation and is presumably involved in DNA repair coupled with DNA replication as well as meiotic DNA recombination in spermatocytes.
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Research Products
(11 results)
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[Publications] Takahashi, E.Matsuda, Y.Hori, T.Yasuda, N.Tsuji, S.Mori, M.Yoshimura, Y.Yamamoto, A.and Morita, T.Matsushiro, A.: "Chromosome mappings of the human (RECA) and mouse (Reca) homologs of the yeast RAD51 and E.coli recA genes to human (15p15.1) and mouse (2F1) chromosomes by direct R-bnading fluorescence in situ hybridization." Genomics. 19. 376-378 (1994)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Kato, T., Todo, T,Ayaki, H,Ishizaki, K,Morita, T., Mitra, S.and Ikenaga, M.: "Cloning of a marsupial DNA photolyase gene and the lack of related nucleotide sequences in placental mammals." Nucleic Acids Res.22. 4119-4124 (1995)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Habu, T., Taki, T., West, A., Nishimune, Y., and Morita, T.: "The mouse and human homologues of DMCq, the yeast meiosis-specific homologous recombination gene, have a common unique form of exon-skipped transcript in meiosis." Nucleic Acids Res.24. 470-477 (1996)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Yamamoto, A.Taki, T., Yagi, H.Babu, T., Yoshida, K.Yoshimura, Y., Yamamot K.Matsudshiro, A., Nishimune, Y,and Morita, T.: "Cell cycle-dependent expression of the mouse Rad51 gene in proliferating cells." Mol.Gen.Genet.(in press). (1996)
Description
「研究成果報告書概要(欧文)」より