1996 Fiscal Year Final Research Report Summary
Development of three-dimensional optical recording methods for neural activity in the central nervous system.
| Project/Area Number |
06558110
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 試験 |
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| Research Field |
神経・脳内生理学
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
KAMINO Kohtaro Tokyo Medical and Dental University, Professor, 医学部, 教授 (40025630)
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| Co-Investigator(Kenkyū-buntansha) |
SATO Katsushige Tokyo Medical and Dental University, Assistant Professor, 医学部, 助手
SAKAI Yoko Tokyo Medical and Dental University, Assistant Professor, 医学部, 助手 (70251501)
SAKAI Tetsuro Tokyo Medical and Dental University, Associate Professor, 医学部, 助教授 (40153845)
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| Project Period (FY) |
1994 – 1996
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| Keywords | optical recording / neural activity / three dimensional imaging / central nervous system / brain stem / 神経核 / 光学切片法 / 脳皮質 |
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| Research Abstract |
In an effort to asses the spatial patterning of glossopharyngeal responses in early embryonic chick brainstem, we used a multiple-site optical recording system with a 12*12 element photodiode array and voltage sensitive merocyanine-rhodanine dyes to monitor neural transmembrane voltage activities. Seven and 8 d old embryonic chick brainstems were sliced into 1400-1600 mum thick sectons with the glossopharyngeal and vagal nerves attached, and then attained withthe dye. Neural voltage-related optical signals were evoked by a positive brief (depolarizing) square current pulse applied to the glossopharyngeal nerve with a microsuction electrode, and then recorded simultaneously from many loci in the objective two-dimensional image plane of a compound microscope. In addition to the multiple-site optical sectioning method by changing the focal plane of the microscope to obtain three-dimensional information. Thus, we have been able to assess semiquantitatively the three-dimensional profiles of two glossopharyngeal response areas corresponding to the nucleus of the glossopharyngeal nerve (nucleus nervi glossopharyngei) and the necleus of the tractus solutarius. Furthermore, glutaminergic excitatory postsynaptic potentials were determined within the response area corresponding to the nucleus of the tractus solitarius. In additon, we also compared the glossopharyngesl and vagal response areas and found that the cores of the related nuclei are separated in three dimensions.
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[Publications] Hirota, A., Sato, K., Momose-Sato, T., Hirota, A., Matsutani, K.& Kamino, K.: "A new simultaneous 1020-site optical recording system for monitoring neural activity using voltage-sensitive dyes." J.Neurosci.Meth.56. 187-194 (1995)
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「研究成果報告書概要(欧文)」より
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[Publications] Momose-Sato, Y., Sato, K., Sasaki, T., Hirota, A., Matsutani, K.& Kamino, K.: "Evaluation of optical voltage-sensitive dyes for optical monitoring of embryonic neural activity." J.Memb.Biol.144. 167-176 (1995)
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「研究成果報告書概要(欧文)」より
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[Publications] Momose-Sato, Y., Sato, K,Sakai, T., Hirota, A., Yang, X.-S,& Kamino, K.: "Optical characterization of a novel GABA response in early embryonic chick brainstem." Neurosci. (in press). (1997)
Description
「研究成果報告書概要(欧文)」より
