1995 Fiscal Year Final Research Report Summary
^1H-NMR Analysis on Histamine Release Mechanism in Mast Cells.
| Project/Area Number |
06670054
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General physiology
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| Research Institution | University of Tokushima |
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Principal Investigator |
YOSHIZAKI Kazuo Sch.Med., Univ.Tokushima, Professor, 医学部, 教授 (50079696)
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| Co-Investigator(Kenkyū-buntansha) |
ARIZONO Naoki Sch.Med., Univ.Kyoto Pref.Univ.Med., Professor, 医学部, 教授 (10079725)
IKEHARA Toshitaka Sch.Med., Univ.Tokushima, Assistant Professor, 医学部, 講師 (40111033)
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| Project Period (FY) |
1994 – 1995
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| Keywords | Mast cells / Histamine / Exocytosis / Compound 48 / 80 / ^1H-NMR / Secretory granule / Lactate |
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| Research Abstract |
1. In ^1H-NMR spectra of intact mast cells two broad resonance lines of histamine were detected. However, these signals were not detected by a presaturation technique. This result indicates that the histamine molecules are in a certain "bound" state in the granules in the cells.
2. When exocytosis was induced by adding compound 48/80 to a suspension of mast cells, sharp resonance lines of free histamine appeared, together with an increase in the resonance of lactate. Thus, exocytosis of granules caused histamine release from the mast cells, accompanied with glycolysis activation.
3. When exocytosis was induced in the cells suspended in an isotonic sucrose solution, we found that the resonance lines of bound histamine remained unchanged in the spectra. When sodium chloride was added into the suspension, the resonance lines of free histamine appeared. The results indicate that the mechanism of histamine release from mast cells consists of two steps. On the initial step exocytosis in the cells causes the release of the granules into the extracellular medium. Then, the histamine molecules are released from the granules, replaced with sodium ions.
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