1995 Fiscal Year Final Research Report Summary
Analysis of interleukin 5 production from mononuclear cells from paragonimiasis patient
Project/Area Number |
06670493
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Research Category |
Grant-in-Aid for General Scientific Research (C)
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Allocation Type | Single-year Grants |
Research Field |
内科学一般
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Research Institution | Kumamoto University |
Principal Investigator |
MIGITA Masahiro School of Medicine Kumamoto Ubiversity Assistant professor, 医学部, 助手 (10244120)
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Project Period (FY) |
1994 – 1995
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Keywords | Interleukin 5(IL-5) / Eosinophilia / Paragonimiasis |
Research Abstract |
(1) In many helminth infections, eosinophils are thought to play a major role in immunopathology of the disease. To elucidate the mechanism of eosinophilia associated with helminth infections, both interleukin-5 (IL-5) producing ability and proliferation of PBMCs from Paragonimus westermani (Pw) -infected and non infected individuals were measured in the presence or absence of helminth antigens. (2) Peripheral blood mononuclear cells (PBMCs) from helminth-infected patients produced greater amounts of IL-5 in vitro, by mitogen stimulation, or Pw specific antigen stimulation than those from nomal controls. Gnathostoma dolotresi (Gd) and Toxocaca canis (Tc) antigens did not stimulate IL-5 secretion by PBMCs from Pw infected individuals. (3) Positive responses to stimulation were detected in PBMCs from patients even seven months after effective treatment, but these responses disappeared at over eleven months. Expansion of IL-5 prodducing cell populations was also demonstrated by PBMCs cultured in the presence of helminth specific antigen. Taken together, these resulfs indicate that patients with helminth infection carry a cell population which can produce IL-5 and expand by stimulation with helminth antigen. Future direction (1) We will isolate mononuclear cells from a patient that produce high amount of IL-5 in vitro and culture them with parasite antigen and IL-2 to produce T cell clones. (2) Analyze T cell clones with monoclonal antibodies against T cells by flow cytometer. (3) Determine and analyze specific antigens which produce IL-5 from T cell clones.
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Research Products
(2 results)