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1995 Fiscal Year Final Research Report Summary

Effect of aging on efficacy of ischemic preconditioning

Research Project

Project/Area Number 06670743
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Circulatory organs internal medicine
Research InstitutionKEIO UNIVERSITY

Principal Investigator

TANI Masato  KEIO UNIV., SCHOOL OF MED., ASSISTANT PROF., 医学部, 講師 (50163613)

Co-Investigator(Kenkyū-buntansha) HASSEGAWA Hiroshi  KEIO UNIV., SCHOOL OF MED.ASSOCIATE, 医学部, 助手 (00237984)
SHINMURA Ken  KEIO UNIV., SCHOOL OF MED.ASSOCIATE, 医学部, 助手 (70206332)
Project Period (FY) 1994 – 1995
Keywordsmyocardium / ischemia / reperfusion / preconditioning / aging / reperfusion injury
Research Abstract

The effects of againg on the recovry of function and metabolites, reperfusion-induced arrhytmias, and sensitivity to PC were investigated in Langendorff-perfused rat hearts. Hearts from young (Y : 12 W/S) or pre-senile (P : 50 W/S) rats were subjected to 15,20, or 25-min global ischemia (GI) followed by 30-min of reperfusion. In some hearts from Y or P,GI was preceded by PC with three 5-min episodes of ischemia with intermittent perfusion. Left ventricular (LV) function (LV pressures, rates of LV pressure development and relaxation) and surface electrocardiogram were monitored during experiments. After perfusion protocol was completed, hearts were frozen and extracted for metabolite assays. There were no differences in cardiac function and myocardial energy metabolites during normoxic perfusion or at the end of ischemia between Y and P.However, recoveries of LV developed pressure, peak positive and peak negative dP/dts (30-50% of Y), and high-energy phoshates (50-60% of Y) were poorer in P with marked increase in LV end-diastolic pressure after anyperiods of GI as compared with those in Y.The incidence of ventricular tachycardia/fibrillation was higher (50% vs.12.5% after 20-min of GI) in P than in Y.PC improved recovery of contractile function (30-50%) and reduced reperfusion arrhythmias in Y whereas no beneficial effects were obtained by the maneuver in P.The results demonstrated that myocardial sensitivity to ischemia-reperfusion injury increased with aging. Furthermore, PC might become less effective with aging.

  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] 谷正人: "Effect of preconditioning on ryanodine-sensitive Ca^<2+> release from sarcoplasmic reticulum of rat heart" Am. J. Physiol. (in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 谷正人: "心筋虚血再灌流傷害感受性に及ぼす加齢の影響" 第38回日本老年医学会学術集会. (in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 谷正人: "Effect of agiong on myocardial sensitivity to ischemia-reperfusion injury and preconditioning(PC)in rat heart" International Society for Heart Research(J. Mol. Cell Cordiol). (in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M. Tani, et al.: "Effect of breef hypoxia on reperfusion arrhyttomias and relacase of Ca^<2+> by rat hearrt homologirate blecked by syandure" Cardiovasc. Res.(in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.TANI: "Effect of preconditioning on ryanodine-sonsitive Ca^<2+> release from sarcoplasmic reticulum of rat heart" Am.J.Physiol. 268 (in press). 1996

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.TANI: "Effect of ageingon sensitivity to ischemia-reperfusion" Jpn.J.Geriat. 33 (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.TANI: "Effect of aging on myocardial sensitivity to is chemia-repmrfusion injury and preconditioning (PC) in rat heart" J.Mol.Cell.Cardiol. (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.TANI: "Effect of brief hypoxia on reperfusion arrhytmias and release of Ca^<2+> by rat heart homogenates blocked by ryanodinc" Cardiovasc.Res. (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1997-03-04  

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