1995 Fiscal Year Final Research Report Summary

Isolation of a novel nephritogenic cationic protein from Streptococcus pyogenes

Research Project

Project/Area Number	06670801
Research Category	Grant-in-Aid for General Scientific Research (C)
Allocation Type	Single-year Grants
Research Field	Pediatrics
Research Institution	TOKUSHIMA UNIVERSITY
Principal Investigator	OKADA Kaname Tokushima University, University Hospital of School of Medicine, Lecturer, 医学部・附属病院, 講師 (10169104)
Co-Investigator(Kenkyū-buntansha)	KURODA Yasuhiro Tokushima University, School of Medicine, Professor, 医学部, 教授 (20035471)
Project Period (FY)	1994 – 1995
Keywords	Cationic protein / Nephritogenic antigen / Streptococcus pyogenes / Glomerulonephritis
Research Abstract	Streptococcal cationic protein (SCP), appearing as a band of 10 kDa on SDS gels and pI>9.0, was purified from the nephritogenic strain of the patients with acute poststreptococcal glomerulonephritis. This protein had a high affinity to the glomerular structure. The rats injected twice with 150 mug of SCP at one week interval developed proteinuria and diffuse mesangial proliferative glomerulonephritis. By immunofluorescence, the deposits of rat IgG and C3 were localized in the mesangium and partly along the capillary wall. No remarkable renal changes were found in the kidney of control rats. These results suggest that SCP may be an unique bacterial antigen which induces glomerulonephritis through in situ immune complex formation.

(2 results)