1995 Fiscal Year Annual Research Report
EPAおよびDHAの乳癌発生、増殖、転移に及ぼす影響およびその機序について
| Project/Area Number |
06671191
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| Research Institution | Kanazawa University |
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Principal Investigator |
野口 昌邦 金沢大学, 医学部・附属病院, 助教授 (20097439)
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| Keywords | 乳癌 / EPA / DHA / MM48 / MDA-MB-231 / Prostaglandin / Leukotriene / MCF-7 |
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| Research Abstract |
In vivoおよびIn vitroの研究で乳癌の発生、増殖、転移に与えるリノール酸、EPAおよびDHAの影響を検討し、次の結果を得た。
(1)リノール酸は、マウスに移植したホルモン非依存性乳癌(MM48)の増殖および転移を促進し、EPAおよびDHAはそれらを抑制した(Kinoshita andNoguchi: Inhibitory effects of purified eicosapentaenoic acid and docosahexaenoic acid on growth and metastasis of murine transplantable mammary tumor. International J of In Vivo Res,8:371-374,1994; Kinoshita, Noguchi and Tanaka: Effects of linoleic acid, eicosapentaenoic acid and docosahezaenoic acid on growth and metastasis of MM48 mammary tumor transplantable in mice. Int J Oncol 8:575-581,1996)
(2)リノール酸は、In vitroでヒト乳癌細胞(MDA-MB-231)のProstaglandin EやLeukotriene Bの分泌、および細胞増殖を促進した。しかし、EPAおよびDHAはそれらを抑制し、EPAの抑制はn-3/n-6比が1:0.69、DHAのそれはn-3/n-6比が1:2.08以上で認められ、更にそれらの抑制は培地中のLeukotriene BよりもProstaglandin Eの濃度と相関することが明かとなった(Noguchi et al: Effects of eicosapentaenoic acid and docosahexaenoic acid on cell growth and prostaglandin E and leukotriene B production by a human breast cancer cell line(MDA-MB-231). Oncology 52:458-464,1995)。
(3)リノール酸は、In vitroでヒト乳癌細胞(MDA-MB-231およびMCF-7)の増殖を促進し、更にMCF-7乳癌細胞ではc-mycの発現を促進することが明かとなった(Earashi, Noguchi et al.: Effects of linoleic acid and eicosanoid synthesis inhibitors on the growth and c-myc oncogene expression of human breast cancer cell. Int J Oncol 8:145-151,1996)。
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[Publications] 江嵐充治・野口昌邦: "Effects of linoleic acid and elcosanoid synthesisinhibitors on the growth and C-myc oncogene expression of human breast cancer cells." Internationl Journal of Oncology. 8. 145-151 (1996)
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[Publications] 江嵐充治・野口昌邦: "In vitro effects of eicosanoid synthesis inhibitors in the presence of linoleic acid on MDA-MB-231 human breast cancer cells." Breast Cancer Research. 37. 29-37 (1996)
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[Publications] 太田長義・野口昌邦: "The effects of high dietary fat and indomethacin on 7.12-dimethylbenz (a)anthracene-induced mammary carcinomas in rats." Oncology Reports. 3. 305-312 (1996)
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[Publications] 木下一夫・野口昌邦: "Effects of linoleic acid,eicosapentaenoic acid,and docosahexaenoic acid on the growth and metastasts of MM48mammary tumor transplantsin mice." Internationl Journal of Oncology. 8. 575-581 (1996)
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[Publications] 野口昌邦: "Effecls of indomethacin with or without linoleic acid on human breast cancer cells in vitro." Prostaglandins Leukotrienes and Essential Fatty Acids.52. 381-386 (1995)
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[Publications] 野口昌邦: "Effects of eicosapentaenoic and docosahexaenoc acid on cell growth and Prostaglandin E and leukotriene B production by a human breast cancer cell line." Oncology. 52. 458-464 (1995)
